scholarly journals Microscopy and Polymerase Chain Reaction Detection ofLeishmania chagasiin the Pleural and Ascitic Fluid of a Patient with AIDS: Case Report and Review of Diagnosis and Therapy of Visceral Leishmaniasis

2004 ◽  
Vol 15 (4) ◽  
pp. 231-234 ◽  
Author(s):  
Ada RS Diehl ◽  
Rodrigo P dos Santos ◽  
Ricardo Zimmerman ◽  
Letícia P Luz ◽  
Tanara Weiss ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Ascitic Fluid ◽  
Clinical Manifestations ◽  
Histological Evaluation ◽  
Diagnostic Tools ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Brazilian Patient ◽  
Polymerase Chain

Atypical visceral leishmaniasis is increasingly reported in immunocompromised patients, including patients with AIDS. A case of visceral leishmaniasis in an HIV-infected Brazilian patient with pulmonary and peritoneal involvement is reported. Histological evaluation of pleural fluid and ascites aspirate revealed macrophages with intracellularLeishmania. Polymerase chain reaction analysis was positive forLeishmaniain the pleural and ascitic fluid with use of primers specific forLeishmania chagasi. In addition to classical methods for diagnosing leishmaniasis, such as microscopy and culture, polymerase chain reaction detection and identification ofLeishmaniaspecies in pleural effusions and ascites are important diagnostic tools that should be considered by clinicians evaluating HIV-infected patients from endemic areas of visceral leishmaniasis. The authors review the clinical manifestations, diagnostic and therapeutic aspects of visceral leishmaniasis in immunocompetent and HIV-infected patients.

scholarly journals Diagnostic Tools for Borrelia Assessment in Humans

2016 ◽  
Vol 10 (1) ◽  
pp. 62-69 ◽  
Author(s):  
Serena Bonin
Keyword(s):  
Polymerase Chain Reaction ◽  
Lyme Disease ◽  
Web Sites ◽  
Clinical Manifestations ◽  
Review Article ◽  
Diagnostic Tools ◽  
Chain Reaction ◽  
Wide Range ◽  
Controversial Topic ◽  
Polymerase Chain

Although the etiological agent of Lyme disease has been known since 1980s, diagnosis of Lyme disease is still a controversial topic because of the wide range of clinical manifestations and the limited diagnostic tools available to assessBorreliain humans.The most used diagnostic tool for Lyme disease is currently serology, but also Polymerase chain reaction (PCR) and other methods are often used to proveBorreliainfection in different patients’ specimens. The present article deals with most of the diagnostic tools used in clinical practice for Lyme disease detection in human samples. Direct and indirect specific methods forBorreliainfection detection will be discussed.The most recent peer reviewed publications as well as original results from our study and information provided by companies’ web sites have been analyzed to compile this review article.

scholarly journals Polymerase Chain Reaction Detection of Lyme Disease:Correlation With Clinical Manifestations and Serologic Responses

1996 ◽  
Vol 105 (5) ◽  
pp. 647-654 ◽  
Author(s):  
C. Lars Mouritsen ◽  
Carl T. Wittwer ◽  
Christine M. Litwin ◽  
Liming Yang ◽  
Janis J. Weis ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Manifestations ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Using a Polymerase Chain Reaction as a Complementary Test to Improve the Detection of Dermatophyte Fungus in Nails

2014 ◽  
Vol 104 (3) ◽  
pp. 233-237 ◽  
Author(s):  
María José Iglesias Sánchez ◽  
Ana María Pérez Pico ◽  
Félix Marcos Tejedor ◽  
María Jesús Iglesias Sánchez ◽  
Raquel Mayordomo Acevedo
Keyword(s):  
Polymerase Chain Reaction ◽  
Dna Sequences ◽  
Classical Method ◽  
Clinical Manifestations ◽  
Culture Method ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Traditional Procedure ◽  
Polymerase Chain

Background Dermatomycoses are a group of pathologic abnormalities frequently seen in clinical practice, and their prevalence has increased in recent decades. Diagnostic confirmation of mycotic infection in nails is essential because there are several pathologic conditions with similar clinical manifestations. The classical method for confirming the presence of fungus in nail is microbiological culture and the identification of morphological structures by microscopy. Methods We devised a nested polymerase chain reaction (PCR) that amplifies specific DNA sequences of dermatophyte fungus that is notably faster than the 3 to 4 weeks that the traditional procedure takes. We compared this new technique and the conventional plate culture method in 225 nail samples. The results were subjected to statistical analysis. Results We found concordance in 78.2% of the samples analyzed by the two methods and increased sensitivity when simultaneously using the two methods to analyze clinical samples. Now we can confirm the presence of dermatophyte fungus in most of the positive samples in just 24 hours, and we have to wait for the result of culture only in negative PCR cases. Conclusions Although this PCR cannot, at present, substitute for the traditional culture method in the detection of dermatophyte infection of the nails, it can be used as a complementary technique because its main advantage lies in the significant reduction of time used for diagnosis, in addition to higher sensitivity.

scholarly journals Polymerase chain reaction detection of cytomegalovirus genome in renal biopsies

1992 ◽  
Vol 42 (4) ◽  
pp. 1012-1016 ◽  
Author(s):  
Susan Kadereit ◽  
Susan Michelson ◽  
Beatrice Mougenot ◽  
Philippe Thibault ◽  
Pierre J. Verroust ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Renal Biopsies ◽  
Polymerase Chain

scholarly journals Polymerase chain reaction detection of sepsis-inducing pathogens in blood using SepsiTest™

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P10 ◽  
Author(s):  
Claudia Disqué ◽  
Anna-Julia Kochem ◽  
Helge Mühl ◽  
Michael Lorenz ◽  
Samir Sakka
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction Detection of Salmonella spp. in Fecal Samples of Pet Birds

2008 ◽  
Vol 3 (1) ◽  
pp. e29-e29
Author(s):  
B. Sareyyüpoğlu ◽  
A Çelik Ok ◽  
Z. Cantekin ◽  
H. Yardimci ◽  
M. Akan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Pet Birds ◽  
Polymerase Chain

scholarly journals Validity of the polymerase chain reaction in the diagnosis of clinically suspected cases of American visceral leishmaniasis

2013 ◽  
Vol 17 (3) ◽  
pp. 319-323
Author(s):  
Celia Maria Silva Pedrosa ◽  
Ricardo Arraes de Alencar Ximenes ◽  
Wendell Alexandre Pinheiro de Almeida ◽  
Eliana Maria Mauricio da Rocha
Keyword(s):  
Polymerase Chain Reaction ◽  
Visceral Leishmaniasis ◽  
Chain Reaction ◽  
Polymerase Chain
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