scholarly journals Establishment of a Protocol for in vitro Seed Germination and Callus Formation of Ficus religiosa L., an Important Medicinal Plant

2018 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Mohsen Hesami ◽  
Mohammad Hosein Daneshvar ◽  
Mohsen Yoosefzadeh-Najafabadi
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Callus Formation ◽  
Ficus Religiosa ◽  
Important Medicinal Plant

scholarly journals Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

2009 ◽  
Vol 61 (1) ◽  
pp. 57-69 ◽  
Author(s):  
Danijela Misic ◽  
B. Siler ◽  
Biljana Filipovic ◽  
Zorica Popovic ◽  
Suzana Zivkovic ◽  
...  
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Salinity Tolerance ◽  
Growth Phase ◽  
In Vitro Selection ◽  
Growth Phases ◽  
Salt Tolerant ◽  
Variable Response ◽  
Selection Of

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

scholarly journals Sexual and Asexual Propagation of Teucrium brevifolium

2018 ◽  
Vol 75 (2) ◽  
Author(s):  
Anastasia AKOUMIANAKI–IOANNIDOU ◽  
Aikaterini GERASIMIDOU ◽  
Alexandra SALTA
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Chemical Treatment ◽  
Stem Cuttings ◽  
Asexual Propagation ◽  
Different Types

The propagation with seeds and stem cuttings of Teucrium brevifolium were investigated in order to facilitate the use of the species in floricultural practice and as a medicinal plant. The seeds after they subjected to different types of treatments (dipping in H2SO4 for 15 min or in H2SO4 for 15 min and GA3 1000 mg l-1 for 10 min, 24 or 48h and untreated) cultured for germination in vitro at 5, 10, 15, 20, 25, and 30 °C. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Seed germination was very low (2%) at temperatures of 15-25 °C, while chemical treatment with the concentrated H2SO4 for 15 min, increased germination to 8-10% at the same temperatures. Asexual propagation of the species resulted in a 52.5-61.5% rooting percentage, in all seasons except winter. Treatment with IBA 2000 mg l-1 increaseds rooting percentage.

scholarly journals Micropropagation of PLUCHEA LANCEOLATA (Oliver & Hiern.) Using Nodal Explant

2014 ◽  
Vol 22 (1) ◽  
pp. 35-39 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Dimpal Joshi ◽  
Sureshkumar Nekkala ◽  
M. Nataraj ◽  
Dharmesh P. Raykundaliya
Keyword(s):  
Medicinal Plant ◽  
Salt Concentration ◽  
Nodal Explants ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Nodal Explant ◽  
Pluchea Lanceolata ◽  
Asteraceae Family ◽  
Important Medicinal Plant

AbstractPluchea lanceolata is an important medicinal plant of Asteraceae family known for its anti-arthritic and anti-inflammatory activity. A protocol was established for micropropagation of P. lanceolata using nodal explants. Nodal explants were inoculated onto Murashige and Skoog (1962) - MS medium supple–mented with 6-benzylaminopurine (BAP), kinetin (Kin), thidiazuron (TDZ) and 2iP (2-isopentenyladenine) at various concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg·dm-3). The highest multiplication rate was obtained for nodal explants cultured on MS medium, supplemented with 0.5 mg·dm-3 thidiazuron (TDZ). In vitro raised shoots were successfully rooted on ½ mineral salt concentration of MS medium supplemented with 1.0 mg dm-3 IBA.

scholarly journals In-vitro Callogenesis and Screening of Antimicrobial Activity of Callus and Seed of Caesalpinia bonducella F.: A Threatened Medicinal Plant of Western Ghats

2021 ◽  
pp. 76-89
Author(s):  
Rajani Shirsat ◽  
Ajit Kengar ◽  
Aruna Rai
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Plant Growth ◽  
Medicinal Plant ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Western Ghats ◽  
Callus Formation ◽  
Caesalpinia Bonducella

Aim: Caesalpinia bonducella Flem is a dioecious scrambling woody liana of Caesalpinoideae, a subfamily of Leguminosae. The plant is threatened and distributed in the deciduous forests of the Western Ghats of India. Being an important medicinal plant Caesalpinia bonducella F. attracted many scientists to exploit various activities associated with a number of phytoconstituents. The present study was undertaken to evaluate the most suitable media and suitable concentrations of plant growth regulators for in vitro Callogenesis and screening of antimicrobial activity of callus and seed of Caesalpinia bonducella. Materials and Methods: Callus was initiated from stem explants, on 1x and 0.5x MS medium plus supplements. The effects of plant growth regulators on callus cultures were studied and observations were made. The in vitro antibacterial activity was performed by using extracts of callus and seed of Caesalpinia bonducella in petroleum ether and methanol against multidrug resistance organisms. The organic extracts of seed and callus of the plant at concentrations of 0.02 mg/ml - 0.1 mg/ml were taken and their activities were measured.   Results: The combination of 2.5 mgL-1 2,4-D  with 2 mgL-1 BAP, resulted in the highest frequency and the highest mean percentage of callus formation (2.35 ± 0.294) with yellow friable callus. The results revealed that all the extracts had a variable degree of antibacterial activity. Conclusions: It was observed that 2,4-D at 2.5 mgL-1 in combination with BAP,2.0 mgL-1 BAP resulted in early initiation, highest induction percentage, with frequency highest mean percentage of callus formation, Antimicrobial tests with methanol and diethyl ether extract of Caesalpinia bonducella seed powder against the clinical isolates showed the zone of inhibition for all the pathogens tested with concentration of methanolic extract of C. bonducella seed powder.

scholarly journals In Vitro Mutation of Capsicum annuum L.var. Kulai by Gamma Radiation

2019 ◽  
Vol 8 (4) ◽  
pp. 6934-6938
Keyword(s):  
Seed Germination ◽  
Gamma Radiation ◽  
Capsicum Annuum ◽  
Shoot Regeneration ◽  
Gamma Ray ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Germination Rate ◽  
Ms Medium

The present work was carried out to investigate the effects of gamma radiation on regeneration of Capsicum annuum L. var Kulai via in vitro. Seeds of C. annuum were irradiated with various doses of gamma ray (0, 20, 40, 60, 80, 100, 200, 300, 400, 500, and 600 Gy) emitted from the Caesium-137 source at the rate of 4.31 Gy per minute. Irradiated seeds grown on MS medium without hormone for hypocotyl and cotyledon preparation as explant for in vitro regeneration. Seed germination rate revealed significant variation between treatments, and seeds started to germinate between 6 to 17 days. Irradiated seeds between 0-60 Gy were observed to germinate in less than 10 days. All explants including hypocotyl and cotyledon were cultured on MS medium with different concentrations of BAP in combination with AgNO3 to observe the response of these explants to different hormone concentrations. From the observation, calluses were induced in 90% of hypocotyl and cotyledon explants in all treatments. The characteristics of calluses were varied with greenish friable, greenish compact, yellowish watery, yellowish friable and yellowish compact. In other treatments, calluses were found in purple, bright yellow and yellowish orange. On the other hand, shoot regeneration was observed in treatment between 40-100 Gy. In conclusion, gamma radiation gave impact on seed germination, seedling growth performance, in vitro callus formation and shoot regeneration of Capsicum annuum var. Kulai

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