scholarly journals Evaluation of Virulence Factors and Antibiotic Resistance Patterns in Clinical Urine Isolates of Klebsiella pneumoniae in Semnan, Iran

2018 ◽  
Vol 11 (7) ◽  
Author(s):  
Ali Jazayeri Moghadas ◽  
Farzaneh Kalantari ◽  
Mohammad Sarfi ◽  
Soroush Shahhoseini ◽  
Shiva Mirkalantari
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Virulence Factors ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Genotypes of Esbl Producing Escherichia Coli and Klebsiella Pneumoniae in Relation to Resistance to Antimicrobial Drugs

PRILOZI ◽  
2014 ◽  
Vol 35 (2) ◽  
pp. 31-38
Author(s):  
Ana Kaftandzieva ◽  
Elena Trajkovska-Dokic ◽  
Vesna Kotevska ◽  
Zaklina Cekovska ◽  
Gordana Jankoska
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Single Gene ◽  
Tracheal Aspirate ◽  
Beta Lactams ◽  
Antimicrobial Drugs ◽  
E Coli ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

Abstract The aim of the study was to evaluate the association of drug resistance with β-lactamase gene types in ESBL positive E. coli and Klebsiella pneumoniae-Kp. Material and methods: A total of 251 ESBL-positive E. coli and Kp isolates obtained from urine, tracheal aspirate, wound swab and blood from patients hospitalised at the University Clinics in Skopje were detected using the ESBL set and automated Vitek 2 system. Vitek was also used for susceptibility testing (determination of MIC of 17 antimicrobial agents). Multiplex PCR was used to identify genes for different types of ESBLs in a 100 randomly selected, ESBL positive strains. Results: More of the 87 ESBL typeable isolates (61%) harbour two or more bla genes and the frequency of antibiotic resistance was high in these isolates, compared to those with a single gene. Isolates with ≥ 3 genes were highly resistant to beta-lactams and non-beta lactams used. The degree of resistance to 3rd generation cephalosporins was also high in these isolates (MIC ≥ 64). More of the ESBL-positive isolates showed higher resistance to cefotaxime than to ceftazidime. Conclusion: Identification of the genes is necessary for the surveillance of their transmission in hospitals. Surveillance of antibiotic resistance patterns are crucial to overcome the problems associated with ESBLs.

scholarly journals 849. Whole Genome Sequencing Analysis of Klebsiella pneumoniae Isolates Reveals Diversity in Genetic Antibiotic Resistance Patterns

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S464-S466
Author(s):  
Ryan Beaver ◽  
Hosoon Choi ◽  
Chetan Jinadatha ◽  
Keith S Kaye ◽  
Piyali Chatterjee ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Genes ◽  
Veterans Affairs ◽  
Whole Genome ◽  
Department Of Veterans Affairs ◽  
Material Support ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns ◽  
Research Grant

Abstract Background Klebsiella pneumoniae is among the leading causes of healthcare-associated infections (HAI). Multidrug-resistant (MDR) Klebsiella variants are difficult to treat and have been reported with increasing frequency in hospitals. Whole genome multi-locus sequence typing (wg-MLST) of K. pneumoniae HAI isolates was used to compare antibiotic resistance genetic patterns against epidemiologic typing. Figure 1. Prevalence of ABX resistance genes for each drug class. Figure 2. Prevalence of ABX resistance genes by hospital. Methods Forty-six clinical bacterial HAI isolates were collected from patients admitted to two disparate tertiary care hospitals in Detroit, Michigan between 2017 and 2019. Data output from wg-MLST was de novo assembled using SPAdes (version 3.7.1) assembler on the Bionumerics calculation engine. Minimum spanning tree (MST) analysis categorized isolates into unique MLST Pasteur serotypes (ST). Antimicrobial resistance genes and/or chromosomal mutations were identified using the ResFinder Database (version 3.2). K. pneumoniae isolates were compared for antibiotic-resistance patterns by hospital, unit, and ST. Figure 3. Hospital 1 clusters and strains that detected gene qnr1, arranged by unit for visual comparison. Figure 4. Sample of clinically significant genes and prevalence, excluding SHV family of genes for simplicity. Results There was significant genetic diversity among the isolates, and no predominant strain was identified. MST analysis revealed 17 unique strains. Only six strains had genetically unique resistance genes detected in more than one isolate, and only three of six were hospital-specific; none were unit-specific. Out of the 75 unique resistance genes detected, only 8 genes had a prevalence >50%: oqxA (100%), oqxB (100%), fosA (89%), blaCTX-M-15 (76%), aac(6’)-Ib-cr (61%), blaTEM-1B (52%), blaOXA-1 (52%), and catB3 (52%). No colistin resistance genes were detected. Of the remaining 69 low-prevalence resistance genes, only 8 hospital-specific genes were detected in more than one isolate (qnrB1, blaSHV-1, blaSHV-110, ac(6’)-Ib3, blaCTX-M-3, blaSHV-36, blaSHV-80, blaSHV-178) with a prevalence range of 4%-22%. Conclusion Our genetic analysis of antibiotic resistance patterns and wg-MLST revealed significant heterogeneity among the isolates, indicating no common source of transmission for either hospital. Although K. pneumoniae is a very common nosocomial pathogen, etiologic analysis suggests diverse community strains (e.g., gut colonization) may actually be responsible for previously-designated HAI. Disclosures Chetan Jinadatha, MD, MPH, AHRQ (Research Grant or Support)Department of Veterans Affairs (Other Financial or Material Support, Owner: Department of Veterans Affairs. Licensed to: Xenex Disinfection System, San Antonio, TX)Inventor (Other Financial or Material Support, Methods for organizing the disinfection of one or more items contaminated with biological agents)NiH/NINR (Research Grant or Support)NSF (Research Grant or Support)Xenex Healthcare Services (Research Grant or Support) Mark Stibich, PhD MHS, Xenex Disinfection Services, Inc (Board Member, Employee)

scholarly journals The Association of the Phylogenetic Typing of the Klebsiella pneumoniae Isolates with Antibiotic Resistance

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Shabnam Baghbanijavid ◽  
Hossein Samadi Kafil ◽  
Safar Farajniya ◽  
Seyed Reza Moaddab ◽  
Hasan Hosainzadegan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Susceptibility Testing ◽  
Fragment Length Polymorphism ◽  
Phylogenetic Groups ◽  
Pcr Rflp ◽  
Resistance Patterns ◽  
Pcr Method ◽  
Antibiotic Resistance Patterns ◽  
The Relationship

Klebsiella pneumoniae complex (KPC) accounts for approximately one-third of all Gram-negative infections. Moreover, it is highly resistant and can taxonomically be distributed into KpI, KpII, and KpIII phylogroups. This study aimed to investigate the distribution of phylogenetic groups and the relationship between them and antibiotic resistance patterns. For this purpose, we collected KPC isolates from Tabriz, Iran, between 2018 and 2020. Antimicrobial susceptibility testing was performed by disk diffusion agar, and phylogenetic groups were then examined using gyrA restriction fragment length polymorphism (RFLP) and parC PCR methods. A total of 100 KPC isolates were obtained from the clinical specimens (urine, respiratory secretion, blood, wounds, and trachea). The enrolled patients included 47 men and 53 women aged from 1 to 91 years old. The highest sensitivity was found related to fosfomycin as 85%, followed by amikacin as 66%. The three phylogenetically groups by the RFLP-PCR method were found in KPC, 96% (96 isolates) as KpI, 3% (3 isolates) as KpII, and 1% (1isolate) as KpIII. The highest antibiotic resistance was observed in KpI. It was shown that a valid identification of three phylogenetic groups of KPC can be done by combining both gyrA PCR-RFLP and parC PCR. Of note, the KpI group was also observed as the dominant phylogenetic group with the highest resistance to antibiotics.

scholarly journals Evaluation of Antibiotic Resistance Patterns of Clinical Klebsiella pneumoniae Isolates from Educational Hospitals in Zahedan, Iran

2018 ◽  
Vol 12 (3) ◽  
pp. 41-45
Author(s):  
Mohammad Bokaeian ◽  
Shahram Shahraki Zahedani ◽  
Abbasali Delarampoor ◽  
Mohammadreza Atashgah ◽  
Bahram Dahmarde ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns

scholarly journals Antibiotic resistance pattern of Klebsiella pneumoniae in obtained samples from Ziaee Hospital of Ardakan, Yazd, Iran during 2016 to 2017

2020 ◽  
Vol 2 (2) ◽  
pp. 32-36
Author(s):  
Jamshid Ayatollahi ◽  
◽  
Mohammad Sharifyazdi ◽  
Razieh Fadakarfard ◽  
Seyed Hossein Shahcheraghi ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Pattern ◽  
Inappropriate Use ◽  
Antibiotic Resistance Pattern ◽  
Appropriate Treatment ◽  
Resistance Patterns ◽  
Susceptibility Tests ◽  
Antibiotic Resistance Patterns

Background: In recent years, due to the inappropriate use of antibiotics, drug resistance has increased in gram negative bacilli, including Klebsiella pneumoniae. Drug resistance is associated with an increase in mortality and therapeutic costs. Therefore, determination of an antibiotic resistance pattern for choosing the appropriate treatment for infections caused by this bacterium seems necessary. This study was conducted to determine the antibiotic resistance pattern of Klebsiella pneumoniae species isolated from patients referring to Ziaee Hospital in Ardakan in 2016-2017. Materials and Methods: For this descriptive-analytic study, all positive cultures of Klebsiella pneumoniae in patients referred to Ziaee Hospital in Ardakan during 2016 to 2017, were evaluated. Antibiotic resistance patterns of the samples were determined by the standard method of propagation of the disk from 12 different antibiotics and data analyzed by SPSS 21 software. Results and discussions: The results of this study, which were performed on 75 samples, showed that 22 (29.3%) were male and 53 (70.7%) were female. Klebsiella's resistance to clarithromycin was 100%, but was 100% susceptible to amikacin. The percentage of Klebsiella's resistance to ampicillin was 78.3%, cefalotin 75%, cotrimoxazole 43.9%, ceftriaxone 32%, ciprofloxacin 30.9%, cefotaxime 24%, and ampicillin 20%. The highest sensitivity of Klebsiella pneumoniae for antibiotics was 100% for amikacin, 82.8% for meropenem and 82% for cefepime. Due to the high prevalence of resistance in Klebsiella samples, there is a need for strict measures in the administration of antibiotics. Antibiotic resistance can also be reduced by choosing the appropriate antibiotic for treatment and by taking antibiotic susceptibility tests.

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