Overview of Genetic Background Beyond Polysaccharide Intercellular Adhesion Production in Staphylococcus epidermidis

2016 ◽  
Vol 10 (1) ◽  
Author(s):  
Mohamed Amine Mekni ◽  
Wafa Achour ◽  
Assia Ben Hassen
Keyword(s):  
Staphylococcus Epidermidis ◽  
Genetic Background ◽  
Intercellular Adhesion ◽  
Polysaccharide Intercellular Adhesion

Purification and Evaluation of Polysaccharide Intercellular Adhesion (PIA) Antigen from Staphylococcus epidermidis

2016 ◽  
Vol 73 (5) ◽  
pp. 611-617 ◽  
Author(s):  
Bahman Mirzaei ◽  
Seyed Fazlollah Moosavi ◽  
Ryhane Babaei ◽  
Seyed Davar Siadat ◽  
Farzam Vaziri ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Intercellular Adhesion ◽  
Polysaccharide Intercellular Adhesion

scholarly journals IgY Targeting Bacterial Quorum-Sensing Molecules in Implant-Associated Infections

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 4027
Author(s):  
Ulrike Dapunt ◽  
Birgit Prior ◽  
Christopher Oelkrug ◽  
Jan Philippe Kretzer
Keyword(s):  
Quorum Sensing ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Major Complication ◽  
Underlying Mechanism ◽  
Bacterial Communication ◽  
Bacterial Quorum Sensing ◽  
Polysaccharide Intercellular Adhesion ◽  
Bacterial Quorum ◽  
Treatment Alternatives

Background: Implant-associated infections are still a major complication in the field of orthopedics. Bacteria can form biofilms on implant surfaces, making them more difficult to detect and treat. Since standard antibiotic therapy is often impaired in biofilm infections, particular interest is directed towards finding treatment alternatives. Biofilm-formation is a well-organized process during which bacteria communicate via quorum-sensing molecules (QSM). The aim of this study was to inhibit bacterial communication by directing avian IgY against specific QSM. Methods: Chicken were immunized against the following QSM: (1) AtlE, a member of the autolysin family which mediates attachment to a surface in Staphylococcus epidermidis; (2) GroEL, the bacterial heat shock protein; (3) PIA (polysaccharide intercellular adhesion), which is essential for cell–cell adhesion in biofilms. Staphylococcus epidermidis biofilms were grown and inhibition of biofilm-formation by IgYs was evaluated. Additionally, human osteoblasts were cultivated and biocompatibility of IgYs was tested. Results: We were able to demonstrate that all IgYs reduced biofilm-formation, also without prior immunization. Therefore, the response was probably not specific with regard to the QSM. Osteoblasts were activated by all IgYs which was demonstrated by microscopy and an increased release of IL-8. Conclusions: In conclusion, avian IgY inhibits biofilm-formation, though the underlying mechanism is not yet clear. However, adverse effects on local tissue cells (osteoblasts) were also observed.

scholarly journals Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion

2019 ◽  
Vol 9 ◽  
Author(s):  
Thirukannamangai Krishnan Swetha ◽  
Murugesan Pooranachithra ◽  
Ganapathy Ashwinkumar Subramenium ◽  
Velayutham Divya ◽  
Krishnaswamy Balamurugan ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Intercellular Adhesion ◽  
Initial Attachment ◽  
Methicillin Resistant

scholarly journals A non‐coding RNA from the intercellular adhesion (ica) locus ofStaphylococcus epidermidiscontrols polysaccharide intercellular adhesion (PIA)‐mediated biofilm formation

2019 ◽  
Vol 111 (6) ◽  
pp. 1571-1591 ◽  
Author(s):  
Maike F. Lerch ◽  
Sonja M.K. Schoenfelder ◽  
Gabriella Marincola ◽  
Freya D.R. Wencker ◽  
Martin Eckart ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Intercellular Adhesion ◽  
Non Coding Rna ◽  
Polysaccharide Intercellular Adhesion

scholarly journals Biofilm characteristics of Staphylococcus epidermidis isolates associated with device-related meningitis

2009 ◽  
Vol 58 (7) ◽  
pp. 855-862 ◽  
Author(s):  
Niall T. Stevens ◽  
Catherine M. Greene ◽  
James P. O'Gara ◽  
Hilary Humphreys
Keyword(s):  
Protein Expression ◽  
Environmental Conditions ◽  
Staphylococcus Epidermidis ◽  
Intercellular Adhesion ◽  
Proteinase K ◽  
Pcr Analysis ◽  
Polysaccharide Intercellular Adhesin ◽  
Rt Pcr ◽  
Sodium Metaperiodate ◽  
Neurosurgical Patients

Staphylococcus epidermidis biofilm causes device-related meningitis in neurosurgical patients. This study assessed the contribution of polysaccharide and protein to the development of a strong biofilm-positive phenotype in four S. epidermidis isolates associated with probable device-related meningitis, under varying environmental conditions. RT-PCR analysis of the intercellular adhesion operon (icaADBC) and assessment of polysaccharide intercellular adhesin (PIA) production indicated a correlation between increased icaA transcription and PIA production in ica + isolates grown in medium with 4 % ethanol and 4 % NaCl. Treatment of biofilm with sodium metaperiodate caused dispersion of adhered cells (P <0.0001), indicating involvement of PIA. Transcriptional levels of protein factors revealed that atlE transcription levels were similar in all isolates, whilst aap levels were variable, with induction being seen in two isolates following growth in the presence of alcohol or salt. Transcription of agr did not influence protein expression and RNAIII transcription varied among the strains. Although aap transcription was induced, the treatment of biofilm with proteinase K did not always disperse the biofilm. Our data suggest that, among the three ica + S. epidermidis isolates clinically associated with meningitis that were studied, PIA contributed to the strong biofilm-positive phenotype, whereas protein factors appeared to have a secondary role.

scholarly journals Polyclonality of Staphylococcus epidermidis residing on the healthy ocular surface

2007 ◽  
Vol 56 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Mayumi Ueta ◽  
Tetsuya Iida ◽  
Masako Sakamoto ◽  
Chie Sotozono ◽  
Junko Takahashi ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Ocular Surface ◽  
Genetic Background ◽  
Healthy Subjects ◽  
Bacterial Flora ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Chromosomal Dna ◽  
Surface Sites ◽  
Normal Bacterial Flora

Staphylococcus epidermidis is part of the normal bacterial flora on the ocular surface. The chromosomal DNA of bacterial isolates obtained from the conjunctival sac, upper and lower lid margins, and upper and lower Meibomian glands of healthy volunteers was subjected to SmaI digestion and PFGE to study the genetic diversity of the organisms. Multiple colonies were also examined of S. epidermidis derived from the conjunctival sac of the same subjects. Lastly, commensal bacteria were harvested from the ocular surfaces of four healthy subjects once a month for 6 months, and the genetic background of the S. epidermidis isolates was analysed. It was found that bacterial strains not only from different subjects but also from multiple ocular surface sites of the same subject exhibited different PFGE patterns. In five of 42 subjects multiple colonies of S. epidermidis were isolated from the conjunctival sac; three harboured multiple colonies with different PFGE patterns, and two manifested multiple colonies with identical PFGE patterns. S. epidermidis isolated from the conjunctival sac of the same subjects over a 6-month period exhibited varying PFGE patterns. The data demonstrate the polyclonality of S. epidermidis on the healthy ocular surface.

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