scholarly journals A non‐coding RNA from the intercellular adhesion (ica) locus ofStaphylococcus epidermidiscontrols polysaccharide intercellular adhesion (PIA)‐mediated biofilm formation

2019 ◽  
Vol 111 (6) ◽  
pp. 1571-1591 ◽  
Author(s):  
Maike F. Lerch ◽  
Sonja M.K. Schoenfelder ◽  
Gabriella Marincola ◽  
Freya D.R. Wencker ◽  
Martin Eckart ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Intercellular Adhesion ◽  
Non Coding Rna ◽  
Polysaccharide Intercellular Adhesion

scholarly journals Evaluation of polysaccharide intercellular adhesion (PIA) and glycerol teichoic acid (Gly-TA) arisen antibodies to prevention of biofilm formation in Staphylococcus aureus and Staphylococcus epidermidis strains

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Sanaz Amir Gholami ◽  
Hamid Reza Goli ◽  
Mohammad Reza Haghshenas ◽  
Bahman Mirzaei
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Teichoic Acid ◽  
Intercellular Adhesion ◽  
Significant Rise ◽  
In Vitro Assays ◽  
Control Group ◽  
Polysaccharide Intercellular Adhesion ◽  
Native Condition

Abstract Objective Staphylococcus aureus and S. epidermidis as opportunistic pathogens, notable for their frequency and severity of infections are recognized as the most usual reasons for medical device-associated infections that strike hospitalized patients and also immunocompromised individuals. In this study, the polysaccharide intercellular adhesion (PIA) and Glycerol teichoic acid) Gly-TA) as two major macromolecules in the biofilm formation process were purified under the native condition and their structure was analyzed by using colorimetric assays and Fourier Transform Infrared spectroscopy (FTIR). Afterward, the immune response of macromolecules and the mixture of them were assessed by measuring total IgG titers. Subsequently, biofilm inhibitory effects of raising antibodies to biofilm former S. aureus and S. epidermidis were evaluated. Results Obtained data were shown a significant rise in levels of antibodies in immunized mice with mentioned antibodies in comparison with the control group. According to the obtained findings, mentioned antibodies could eliminate S. aureus and S. epidermidis biofilm formation in vitro assays. This survey confirms the proposal that immunization of mice with a mixture of Gly-TA and PIA vaccine could be secure and protected against S. epidermidis and S. aureus infection.

scholarly journals IgY Targeting Bacterial Quorum-Sensing Molecules in Implant-Associated Infections

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 4027
Author(s):  
Ulrike Dapunt ◽  
Birgit Prior ◽  
Christopher Oelkrug ◽  
Jan Philippe Kretzer
Keyword(s):  
Quorum Sensing ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Major Complication ◽  
Underlying Mechanism ◽  
Bacterial Communication ◽  
Bacterial Quorum Sensing ◽  
Polysaccharide Intercellular Adhesion ◽  
Bacterial Quorum ◽  
Treatment Alternatives

Background: Implant-associated infections are still a major complication in the field of orthopedics. Bacteria can form biofilms on implant surfaces, making them more difficult to detect and treat. Since standard antibiotic therapy is often impaired in biofilm infections, particular interest is directed towards finding treatment alternatives. Biofilm-formation is a well-organized process during which bacteria communicate via quorum-sensing molecules (QSM). The aim of this study was to inhibit bacterial communication by directing avian IgY against specific QSM. Methods: Chicken were immunized against the following QSM: (1) AtlE, a member of the autolysin family which mediates attachment to a surface in Staphylococcus epidermidis; (2) GroEL, the bacterial heat shock protein; (3) PIA (polysaccharide intercellular adhesion), which is essential for cell–cell adhesion in biofilms. Staphylococcus epidermidis biofilms were grown and inhibition of biofilm-formation by IgYs was evaluated. Additionally, human osteoblasts were cultivated and biocompatibility of IgYs was tested. Results: We were able to demonstrate that all IgYs reduced biofilm-formation, also without prior immunization. Therefore, the response was probably not specific with regard to the QSM. Osteoblasts were activated by all IgYs which was demonstrated by microscopy and an increased release of IL-8. Conclusions: In conclusion, avian IgY inhibits biofilm-formation, though the underlying mechanism is not yet clear. However, adverse effects on local tissue cells (osteoblasts) were also observed.

Purification and Evaluation of Polysaccharide Intercellular Adhesion (PIA) Antigen from Staphylococcus epidermidis

2016 ◽  
Vol 73 (5) ◽  
pp. 611-617 ◽  
Author(s):  
Bahman Mirzaei ◽  
Seyed Fazlollah Moosavi ◽  
Ryhane Babaei ◽  
Seyed Davar Siadat ◽  
Farzam Vaziri ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Intercellular Adhesion ◽  
Polysaccharide Intercellular Adhesion

scholarly journals Umbelliferone Impedes Biofilm Formation and Virulence of Methicillin-Resistant Staphylococcus epidermidis via Impairment of Initial Attachment and Intercellular Adhesion

2019 ◽  
Vol 9 ◽  
Author(s):  
Thirukannamangai Krishnan Swetha ◽  
Murugesan Pooranachithra ◽  
Ganapathy Ashwinkumar Subramenium ◽  
Velayutham Divya ◽  
Krishnaswamy Balamurugan ◽  
...  
Keyword(s):  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Intercellular Adhesion ◽  
Initial Attachment ◽  
Methicillin Resistant

scholarly journals Staphylococcus aureus Fibronectin-Binding Protein A Mediates Cell-Cell Adhesion through Low-Affinity Homophilic Bonds

mBio ◽  
2015 ◽  
Vol 6 (3) ◽  
Author(s):  
Philippe Herman-Bausier ◽  
Sofiane El-Kirat-Chatel ◽  
Timothy J. Foster ◽  
Joan A. Geoghegan ◽  
Yves F. Dufrêne
Keyword(s):  
Staphylococcus Aureus ◽  
Cell Surface ◽  
Biofilm Formation ◽  
Protein A ◽  
Intercellular Adhesion ◽  
Surface Proteins ◽  
Content Type ◽  
Fibronectin Binding ◽  
A Domains ◽  
Cell Cell

ABSTRACT Staphylococcus aureus is an important opportunistic pathogen which is a leading cause of biofilm-associated infections on indwelling medical devices. The cell surface-located fibronectin-binding protein A (FnBPA) plays an important role in the accumulation phase of biofilm formation by methicillin-resistant S. aureus (MRSA), but the underlying molecular interactions are not yet established. Here, we use single-cell and single-molecule atomic force microscopy to unravel the mechanism by which FnBPA mediates intercellular adhesion. We show that FnBPA is responsible for specific cell-cell interactions that involve the FnBPA A domain and cause microscale cell aggregation. We demonstrate that the strength of FnBPA-mediated adhesion originates from multiple low-affinity homophilic interactions between FnBPA A domains on neighboring cells. Low-affinity binding by means of FnBPA may be important for biofilm dynamics. These results provide a molecular basis for the ability of FnBPA to promote cell accumulation during S. aureus biofilm formation. We speculate that homophilic interactions may represent a generic strategy among staphylococcal cell surface proteins for guiding intercellular adhesion. As biofilm formation by MRSA strains depends on proteins rather than polysaccharides, our approach offers exciting prospects for the design of drugs or vaccines to inhibit protein-dependent intercellular interactions in MRSA biofilms. IMPORTANCE Staphylococcus aureus is a human pathogen that forms biofilms on indwelling medical devices, such as central venous catheters and prosthetic joints. This leads to biofilm infections that are difficult to treat with antibiotics because many cells within the biofilm matrix are dormant. The fibronectin-binding proteins (FnBPs) FnBPA and FnBPB promote biofilm formation by clinically relevant methicillin-resistant S. aureus (MRSA) strains, but the molecular mechanisms involved remain poorly understood. We used atomic force microscopy techniques to demonstrate that FnBPA mediates cell-cell adhesion via multiple, low-affinity homophilic bonds between FnBPA A domains on adjacent cells. Therefore, FnBP-mediated homophilic interactions represent an interesting target to prevent MRSA biofilms. We propose that such homophilic mechanisms may be widespread among staphylococcal cell surface proteins, providing a means to guide intercellular adhesion and biofilm accumulation.

scholarly journals Biofilm Formation and the Presence of the Intercellular Adhesion Locus ica among Staphylococci from Food and Food Processing Environments

2003 ◽  
Vol 69 (9) ◽  
pp. 5648-5655 ◽  
Author(s):  
Trond Møretrø ◽  
Lene Hermansen ◽  
Askild L. Holck ◽  
Maan S. Sidhu ◽  
Knut Rudi ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Sodium Chloride ◽  
Northern Blot ◽  
Food Processing ◽  
Biofilm Formation ◽  
Food Industry ◽  
Southern Blotting ◽  
Northern Blot Analysis ◽  
Intercellular Adhesion ◽  
Ammonium Compounds

ABSTRACT In clinical staphylococci, the presence of the ica genes and biofilm formation are considered important for virulence. Biofilm formation may also be of importance for survival and virulence in food-related staphylococci. In the present work, staphylococci from the food industry were found to differ greatly in their abilities to form biofilms on polystyrene. A total of 7 and 21 of 144 food-related strains were found to be strong and weak biofilm formers, respectively. Glucose and sodium chloride stimulated biofilm formation. The biofilm-forming strains belonged to nine different coagulase-negative species of Staphylococcus. The icaA gene of the intercellular adhesion locus was detected by Southern blotting and hybridization in 38 of 67 food-related strains tested. The presence of icaA was positively correlated with strong biofilm formation. The icaA gene was partly sequenced for 22 food-related strains from nine different species of Staphylococcus, and their icaA genes were found to have DNA similarities to previously sequenced icaA genes of 69 to 100%. Northern blot analysis indicated that the expression of the ica genes was higher in strong biofilm formers than that seen with strains not forming biofilms. Biofilm formation on polystyrene was positively correlated with biofilm formation on stainless steel and with resistance to quaternary ammonium compounds, a group of disinfectants.

scholarly journals Clinical significance of the level of intercellular adhesion molecules 1 in patients with Candida stomatitis depending on the ability of Candida spp to foms biofilms

2020 ◽  
pp. 52-57
Author(s):  
D Novikov ◽  
A Pozharitskaya ◽  
I Karpuk
Keyword(s):  
Adhesion Molecules ◽  
Oral Mucosa ◽  
Biofilm Formation ◽  
Oral Fluid ◽  
Oral Candidiasis ◽  
Intercellular Adhesion ◽  
Control Group ◽  
Microbiological Method ◽  
Candida Spp ◽  
Intercellular Adhesion Molecules

Aim: to assess the level of intercellular adhesion molecules 1 (ICAM-1) in the oral fluid of patients with candidal stomatitis, depending on the ability of fungi Candida to form a biofilm. The object of the study were 67 patients with oral mucosa candidiasis and 23 patients of the control group without oral candidiasis. A clinical examination of 90 patients was carried out, smears were taken to confirm the diagnosis by a microbiological method, ELISA to assess the level of ICAM-1 in the oral fluid, to determine the biofilm-forming ability of strains of fungi Candida. According to the results of the study, in 41 (61,2%) patients with candidal stomatitis, strains of fungi Candida had the ability to form a biofilm, and in 26 (38,8%) patients this ability was absent, and in 19 patients (46,3%) it was low ability to biofilm formation, in 21 (51,3%) - moderate ability and in 1 (2,4%) - high. In patients with candidiasis stomatitis, the level of ICAM-1 concentration in saliva was 8,51 ± 0,5 ng / ml, which significantly distinguished it (p <0,001) from the indicator in the control group without oral mucosa candidiasis (4,51 ± 0,32 ng / ml). In patients with candidal stomatitis (n = 67), the level of ICAM-1 in saliva was significantly higher (p <0,01) in the group with biofilm-forming strains than with biofilm-non-forming strains.

Sign in / Sign up

Export Citation Format

Share Document