scholarly journals Antibacterial Efficacy of Super-Oxidized Water on Enterococcus faecalis Biofilms in Root Canal

2016 ◽  
Vol 9 (9) ◽  
Author(s):  
Recai Zan ◽  
Tayfun Alacam ◽  
Ihsan Hubbezoglu ◽  
Tutku Tunc ◽  
Zeynep Sumer ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Efficacy

scholarly journals Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study

2017 ◽  
Vol 6 (2) ◽  
pp. 65-67
Author(s):  
Neha Saini ◽  
Abhinav C Singhal ◽  
Aditi S Jain ◽  
Jayesh Tiwari ◽  
Pratik Surana
Keyword(s):  
Enterococcus Faecalis ◽  
Green Tea ◽  
Root Canal ◽  
Antibacterial Efficacy ◽  
Herbal Extracts ◽  
Zone Of Inhibition ◽  
Canal System ◽  
Root Canal System ◽  
Tea Extract

ABSTRACT Introduction The main objective of root canal treatment is to disinfect the entire root canal system, and irrigation is an important step in reducing the bacterial load from the root canal system. However, irrigants currently used in the field of endodontics have their share of limitations, and the search for an ideal root canal irrigant continues. The use of herbal extracts as endodontic irrigants is today gaining popularity. Aims and objectives To evaluate and compare the antimicrobial potential of herbal extracts, such as neem (Azadirachta indica), triphala (Terminalia chebula), green tea (Camellia sinensis), and combination of neem and triphala as endodontic irrigants against Enterococcus faecalis. Materials and methods Freshly prepared extracts of neem, triphala, green tea, and 2% chlorhexidine were used to assess the antimicrobial efficiency against E. faecalis using the agar well diffusion test. Agar plates were incubated at 370°C for 24 hours in an incubator. The diameter of bacterial inhibition zones around each well was recorded to the nearest size in mm. Results Higher mean zone of inhibition was recorded in chlorhexidine followed by neem extract and combination of neem and triphala than triphala and green tea extract respectively. The lowest mean zone of inhibition was found in green tea extract. Conclusion From the present study, it can be concluded that neem leaf extract shows comparable zones of inhibition with that of chlorhexidine and combination of neem and triphala. How to cite this article Singhal AC, Jain AS, Tiwari J, Surana P, Saini N. Antibacterial Efficacy of Neem, Triphala, Green Tea, and Combination of Neem with Triphala Extract against Enterococcus faecalis: An in vitro Study. Int J Experiment Dent Sci 2017;6(2):65-67.

scholarly journals Evaluation of Antibacterial Efficacy of Vitex negundo Linn. extract as Root Canal Irrigant against Enterococcus faecalis and its penetration into Root Dentin: An in-vitro study

2020 ◽  
Vol 11 (2) ◽  
pp. 193-199
Author(s):  
Suruchi Santosh Gupta ◽  
Nilima Thosar ◽  
Nilesh Rathi ◽  
Sudhindra M Baliga ◽  
Yagnesh Thakkar ◽  
...  
Keyword(s):  
Penetration Depth ◽  
Enterococcus Faecalis ◽  
Root Canal ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Antibacterial Efficacy ◽  
Vitex Negundo ◽  
Root Dentin ◽  
Root Canal Irrigant

Introduction: The aim of this study was to evaluate the antibacterial efficacy of Vitex negundo Linn. extract as root canal irrigant against Enterococcus faecalis and its penetration into root dentin. Methods and Materials: Forty single rooted premolars were randomly divided into 4 groups: 3% Sodium hypochlorite (NaOCl), 2% Chlorhexidine (CHX) , 100mg/ml Vitex negundo Linn. and saline as control all mixed with Rhodamine B dye. Test samples were analysed for bacterial count before and after irrigation using absorbent paper points and the colony forming units were recorded and measured. Sectioning of the samples was performed at three levels 3mm,6mm,9mm from apex and then these samples were analysed using confocal laser scanning microscopy for penetration depth of the irrigant within the dentinal tubules. Paired t-test and ANOVA test were used to perform statistical analysis with level of significance set at 0.05 Results: The mean CFU/ml count of Enterococcus facealis reduced significantly in all the groups post irrigation. All the irrigants showed maximum penetration depth at coronal third level compared to middle and apical third level respectively. The penetration depth of NaOCl group was better when compared to CHX group and Vitex negundo Linn. group but the difference was statistically not significant. Conclusion: Although 3% NaOCl was the most effective irrigant, all agents exerted acceptable antimicrobial activity against Enterococcus faecalis and penetration depth within tubules of dentin.

scholarly journals Short-Term Antibacterial Efficacy of Three Bioceramic Root Canal Sealers Against Enterococcus Faecalis Biofilms

2020 ◽  
Vol 54 (1) ◽  
pp. 3-9
Author(s):  
Marija Šimundić Munitić ◽  
Ana Budimir ◽  
Suzana Jakovljević ◽  
Ivica Anić ◽  
Ivona Bago
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Antibacterial Efficacy ◽  
Short Term ◽  
Root Canal Sealers

scholarly journals Use of electromagnetic stimulation on an Enterococcus faecalis biofilm on root canal treated teeth in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz H. D. Panariello ◽  
Justin K. Kindler ◽  
Kenneth J. Spolnik ◽  
Ygal Ehrlich ◽  
George J. Eckert ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Laser Scanning ◽  
Confocal Imaging ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Microscopy Imaging ◽  
Electromagnetic Stimulation ◽  
Root Canal Disinfection

AbstractRoot canal disinfection is of utmost importance in the success of the treatment, thus, a novel method for achieving root canal disinfection by electromagnetic waves, creating a synergistic reaction via electric and thermal energy, was created. To study electromagnetic stimulation (EMS) for the disinfection of root canal in vitro, single rooted teeth were instrumented with a 45.05 Wave One Gold reciprocating file. Specimens were sterilized and inoculated with Enterococcus faecalis ATCC 29,212, which grew for 15 days to form an established biofilm. Samples were treated with 6% sodium hypochlorite (NaOCl), 1.5% NaOCl 1.5% NaOCl with EMS, 0.9% saline with EMS or 0.9% saline. After treatments, the colony forming units (CFU) was determined. Data was analyzed by Wilcoxon Rank Sums Test (α = 0.05). One sample per group was scored and split for confocal laser scanning microscopy imaging. There was a significant effect with the use of NaOCl with or without EMS versus 0.9% saline with or without EMS (p = 0.012 and 0.003, respectively). CFUs were lower when using 0.9% saline with EMS versus 0.9% saline alone (p = 0.002). Confocal imaging confirmed CFU findings. EMS with saline has an antibiofilm effect against E. faecalis and can potentially be applied for endodontic disinfection.

scholarly journals Antimicrobial Efficacy of Propolis in Comparison to Chlorhexidine against Enterococcus faecalis: A Systematic Review and Meta-Analysis

2021 ◽  
Vol 11 (8) ◽  
pp. 3469
Author(s):  
Khalid H. Almadi ◽  
Muhammad Adeel Ahmed ◽  
Tuba Ghazal ◽  
Rizwan Jouhar ◽  
Mazen F. Alkahtany ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Meta Analysis ◽  
Mean Difference ◽  
Permanent Teeth ◽  
Antibacterial Efficacy ◽  
Chi Square ◽  
Reporting Standard ◽  
Standard Mean Difference ◽  
Significant Difference ◽  
Chi Square Test

Propolis is proposed to possess antibacterial and anti-inflammatory properties, which can be used in endodontic applications. However, evidence on its efficacy in comparison to chlorhexidine against Enterococcus faecalis (E. faecalis) is controversial. The aim of the current study was to compare the antibacterial efficacy of Propolis and chlorhexidine as an intracanal medicament against E. faecalis in extracted human permanent teeth. The focused question was, “Does Propolis show better antibacterial efficacy than Chlorhexidine (CHX) as an intracanal medicament against E. faecalis in extracted human permanent teeth?”. Databases including PubMed/Medline, Scopus, EMBASE, ISI-Web of Science were searched from 1990 to August 2020 using different combinations of the following keywords: “Propolis”, “Intracanal medicament”, “E. faecalis”, “Antibacterial activity” and “Chlorhexidine”. Ten studies fulfilling inclusion criteria were considered for qualitative analysis, followed by quantitative analysis of eight studies. Heterogeneity was calculated for colony forming units (CFU) of E. Faecalis using the Chi-square test and I2 statistics. Forest plots were computed reporting standard mean difference (SMD) of outcomes and 95% confidence intervals. The overall mean difference for CFU of E. faecalis showed a statistically significant difference between the antibacterial efficacy of Propolis and CHX (SMD = 3.20 [1.70, 4.69] Z = 4.20; p < 0.001). CHX showed superior antibacterial efficacy against E. faecalis compared to Propolis.

scholarly journals Effect of Antibacterial Root Canal Sealer on Persistent Apical Periodontitis

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 741
Author(s):  
Zheng Wang ◽  
Ge Yang ◽  
Biao Ren ◽  
Yuan Gao ◽  
Xian Peng ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Therapeutic Effect ◽  
Root Canal ◽  
Apical Periodontitis ◽  
Control Group ◽  
Root Canal Sealer ◽  
Root Canal Filling ◽  
First Time ◽  
Cone Beam Computer Tomography ◽  
Better Than

The infection of Enterococcus faecalis and its interacting microorganisms in the root canal could cause persistent apical periodontitis (AP). Antibacterial root canal sealer has favorable prospects to inhibit biofilms. The purpose of this study was to investigated the antibacterial effect of root canal sealer containing dimethylaminododecyl methacrylate (DMADDM) on persistent AP in beagle dogs for the first time. Persistent AP was established by a two-step infection with Enterococcus faecalis and multi-bacteria (Enterococcus faecalis, Lactobacillus acidophilus, Actinomycesnaeslundii, Streptococcus gordonii). Root canal sealer containing DMADDM (0%, 1.25%, 2.5%) was used to complete root canal filling. The volume of lesions and inflammatory grade in the apical area were evaluated by cone beam computer tomography (CBCT) and hematoxylin-eosin staining. Both Enterococcus-faecalis- and multi-bacteria-induced persistent AP caused severe apical destruction, and there were no significant differences in pathogenicity between them. DMADDM-modified sealer significantly reduced the volume of periapical lesion and inflammatory grade compared with the control group, among them, the therapeutic effect of the 2.5% group was better than the 1.25% group. In addition, E.faecalis-induced reinfection was more sensitive to the 2.5% group than multi-bacteria reinfection. This study shows that root canal sealer containing DMADDM had a remarkable therapeutic effect on persistent AP, especially on E. faecalis-induced reinfection.

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