Molecular Analysis of Pseudomonas aeruginosa Strains Isolated from Burn Patients by Repetitive Extragenic Palindromic-PCR (rep-PCR)

2017 ◽  
Vol 19 (4) ◽  
Author(s):  
Mansooreh Abbassi Ghaleh Sorkh ◽  
Leili Shokoohizadeh ◽  
Niloufar Rashidi ◽  
Elahe Tajbakhsh
Burns ◽  
2016 ◽  
Vol 42 (5) ◽  
pp. 1116-1120 ◽  
Author(s):  
Azar Dokht Khosravi ◽  
Fatemeh Shafie ◽  
Effat Abbasi Montazeri ◽  
Soodabeh Rostami

2015 ◽  
Vol 8 (10) ◽  
Author(s):  
Zahra Ghanbarzadeh Corehtash ◽  
Ahmad Khorshidi ◽  
Farzaneh Firoozeh ◽  
Hosein Akbari ◽  
Azam Mahmoudi Aznaveh

2005 ◽  
Vol 71 (10) ◽  
pp. 5992-5998 ◽  
Author(s):  
Zexun Lu ◽  
David Lapen ◽  
Andrew Scott ◽  
Angela Dang ◽  
Edward Topp

ABSTRACT Repetitive extragenic palindromic PCR fingerprinting of Escherichia coli is one microbial source tracking approach for identifying the host source origin of fecal pollution in aquatic systems. The construction of robust known-source libraries is expensive and requires an informed sampling strategy. In many types of farming systems, waste is stored for several months before being released into the environment. In this study we analyzed, by means of repetitive extragenic palindromic PCR using the enterobacterial repetitive intergenic consensus primers and comparative analysis using the Bionumerics software, collections of E. coli obtained from a dairy farm and from a swine farm, both of which stored their waste as a slurry in holding tanks. In all fecal samples, obtained from either barns or holding tanks, the diversity of the E. coli populations was underrepresented by collections of 500 isolates. In both the dairy and the swine farms, the diversity of the E. coli community was greater in the manure holding tank than in the barn, when they were sampled on the same date. In both farms, a comparison of stored manure samples collected several months apart suggested that the community composition changed substantially in terms of the detected number, absolute identity, and relative abundance of genotypes. Comparison of E. coli populations obtained from 10 different locations in either holding tank suggested that spatial variability in the E. coli community should be accounted for when sampling. Overall, the diversity in E. coli populations in manure slurry storage facilities is significant and likely is problematic with respect to library construction for microbial source tracking applications.


2020 ◽  
Vol Volume 13 ◽  
pp. 2261-2275
Author(s):  
Hajar Mohammadi Barzelighi ◽  
Bita Bakhshi ◽  
Bahram Daraei ◽  
Hossein Fazeli ◽  
Bahram Nasr Esfahani

2012 ◽  
Vol 5 (3) ◽  
pp. 507-510 ◽  
Author(s):  
Enayatollah Kalantar ◽  
Vahideh Torabi ◽  
Himen Salimizand ◽  
Fariborz Soheili ◽  
Rashid Ramezanzadeh

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