scholarly journals Hematological Parameters in Adult Patients with COVID-19; A Case-Control Study

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sawer Sabri Ahmed ◽  
Dilveen Ahmed Mohammed ◽  
Ayad Ahmad Mohammed
Keyword(s):  
Hematological Parameters ◽  
Young Patients ◽  
Blood Parameters ◽  
Health Concern ◽  
Rt Pcr ◽  
Aged Patients ◽  
The Mean ◽  
Polymerase Chain ◽  
Symptoms And Signs ◽  
Control Study

Background: The coronavirus disease 2019 (COVID-19) pandemic is a serious public health concern worldwide. The virus mainly causes respiratory symptoms, but its manifestations might be different. Assessments showed that COVID-19 can cause various alterations in the blood parameters. Objectives: This study aimed to detect the most common changes in the blood parameters in COVID-19 patients and compare them with healthy individuals. Methods: In this study, 350 individuals were grouped into two equal groups of COVID‐19 patients and healthy controls. Results: The mean age of patients was 53.73 years, and males constituted 52.57% of them. Middle aged patients constituted about 55.43% of participants, elderly patients 26.29%, and young patients 18.29%. There was a significant correlation between COVID-19 and all the blood parameters, including Hb, MCV, WBC, neutrophils, lymphocytes, monocytes, eosinophils, and platelets (P = 0.000). Conclusions: Infection with COVID-19 is associated with multisystem symptoms and signs, as well as numerous laboratory abnormalities. An increased focus should be on changes in the blood parameters as valuable markers in suggesting the infection when the reverse transcription polymerase chain reaction (RT-PCR) is negative. These parameters can give clues about the severity of the infection and predict the admission to the intensive care unit (ICU).

Kingella kingae septic arthritis

2021 ◽  
Vol 103-B (3) ◽  
pp. 584-588
Author(s):  
Mohammed Khattak ◽  
Sujith Vellathussery Chakkalakumbil ◽  
Robert A. Stevenson ◽  
David J. Bryson ◽  
Michael J. Reidy ◽  
...  
Keyword(s):  
Septic Arthritis ◽  
Young Patients ◽  
Case Series ◽  
Blood Parameters ◽  
Pcr Analysis ◽  
Kingella Kingae ◽  
Patient Demographics ◽  
The Mean ◽  
Polymerase Chain ◽  
Prospective Case Series

Aims The aim of this study was to determine the extent to which patient demographics, clinical presentation, and blood parameters vary in Kingella kingae septic arthritis when compared with those of other organisms, and whether this difference needs to be considered when assessing children in whom a diagnosis of septic arthritis is suspected. Methods A prospective case series was undertaken at a single UK paediatric institution between October 2012 and November 2018 of all patients referred with suspected septic arthritis. We recorded the clinical, biochemical, and microbiological findings in all patients. Results A total of 160 patients underwent arthrotomy for a presumed septic arthritis. Of these, no organism was identified in 61 and only 25 of these were both culture- and polymerase chain reaction (PCR)-negative. A total of 36 patients did not undergo PCR analysis. Of the remaining 99 culture- and PCR-positive patients, K. kingae was the most commonly isolated organism (42%, n = 42). The knee (n = 21), shoulder (n = 9), and hip (n = 5) were the three most commonly affected joints. A total of 28 cases (66%) of K. kingae infection were detected only on PCR. The mean age of K. kingae-positive cases (16.1 months) was significantly lower than that of those whose septic arthitis was due to other organisms (49.4 months; p < 0.001). The mean CRP was significantly lower in the K. kingae group than in the other organism group (p < 0.001). The mean ESR/CRP ratio was significantly higher in K. kingae (2.84) than in other infections (1.55; p < 0.008). The mean ESR and ESR/CRP were not significantly different from those in the 'no organism identified' group. Conclusion K. kingae was the most commonly isolated organism from paediatric culture- and/or PCR-positive confirmed septic arthritis, with only one third of cases detected on routine cultures. It is important to develop and maintain a clinical suspicion for K. kingae infection in young patients presenting atypically. Routine PCR testing is recommended in these patients. Cite this article: Bone Joint J 2021;103-B(3):584–588.

Detection of Bovine Ephemeral Fever Virus and Its Effects on Blood Parameters and Serum Calcium Levels in Cattle Population of District Swabi, Pakistan

2020 ◽  
Author(s):  
Sahibzada Waheed Abdullah ◽  
Muti Ur- Rehman Khan ◽  
Asim Aslam ◽  
Saima Masood ◽  
Amir Ghafoor Bajwa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hematological Parameters ◽  
Blood Parameters ◽  
Fever Virus ◽  
Rt Pcr ◽  
Bovine Ephemeral Fever Virus ◽  
Chain Reaction ◽  
Blood Samples ◽  
Bovine Ephemeral Fever ◽  
Polymerase Chain

Bovine ephemeral fever (BEF) is a fatal viral disease predominantly affecting cattle and buffaloes. Infection results in a huge economic loss, especially due to the reduction in milk production. In Pakistan. There is a dearth of information on bovine ephemeral fever. The study was designed to detect and examine the effect of bovine ephemeral fever virus on hematological parameters among cattle in Swabi district, Khyber Pakhtunkhwa, Pakistan. A total of fifty blood samples were collected from suspected cattle and scrutinized for bovine ephemeral fever virus, using nested Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Hematological parameters were analyzed using hematology and chemistry analyzer. Of the 50 blood samples, 33 representing 66% were positive for bovine ephemeral fever virus through RT-PCR. A product size of 809 bp was observed in the reaction-I, while 505bp was obtained in reaction-II. Neutrophils of infected cattle were significantly increased (10.44 ± 1.87 × 109/L) (plessthan 0.05), with a significant decrease plessthan 0.05) in lymphocytes levels (2.90 ± 0.97 × 109/L). Changes in other blood parameters were non-significant. Furthermore, there was also a significant decrease (plessthan 0.05) in serum calcium level (7.84 ± 0.16 mg/dL). We confirmed the detection of bovine ephemeral fever virus by nested reverse transcriptase polymerase chain reaction in Swabi, Khyber Pakhtunkhwa, Pakistan.

scholarly journals Olfactory and Gustatory Dysfunctions as a Clinical Manifestation of Coronavirus Disease 2019 in a Malaysian Tertiary Center

2020 ◽  
pp. 000348942096316 ◽  
Author(s):  
Kuganathan Ramasamy ◽  
Jeyasakthy Saniasiaya ◽  
Norhaslinda Abdul Gani
Keyword(s):  
Nasal Obstruction ◽  
Telephone Interview ◽  
Complete Recovery ◽  
Sudden Onset ◽  
Olfactory Dysfunction ◽  
Rt Pcr ◽  
Younger Age ◽  
Tertiary Center ◽  
The Mean ◽  
Polymerase Chain

Objective: To investigate the prevalence of olfactory and gustatory dysfunction among patients with COVID-19 infection and the recovery rate. Methods: Adult patients (≥18 years) tested positive for COVID-19 via reverse transcription-polymerase chain reaction (RT-PCR) and admitted in Hospital Tuanku Ja’afar Seremban, Malaysia, were recruited in this study. Patients completed a questionnaire via telephone interview comprising the following details: age, sex, ethnicity, comorbidities, general and otorhinolaryngological symptoms, onset and duration of olfactory and gustatory dysfunction. Patients with persistent olfactory and gustatory dysfunction at the time of the initial interview were followed-up every 3 to 5 days till resolution. Results: A total of 145 patients were included in our study. The mean age of patients was 43.0 ± 17.7 (range: 18-86). Fever (44.1%) and cough (39.3%) were the most prevalent general symptoms. Thirty-one patients (21.4%) reported olfactory dysfunction and 34 (23.4%) reported dysgeusia. There was a significant association between both olfactory and gustatory dysfunction ( P < .001). Altered sense of smell or taste occurred before other symptoms in 7 (15.9%); concomitant in 16 (36.4%) and after in 15 (34.1%). Six patients (13.6%) reported isolated sudden-onset anosmia. The median duration of olfactory and gustatory dysfunctions was 7 days. Complete recovery was achieved for 70.5% of the patients within 7 days of symptom onset. Only 6 (19.4%) of the 31 patients with olfactory dysfunction experienced nasal obstruction or rhinorrhea. Olfactory dysfunction was not significantly associated with nasal obstruction or rhinorrhea. Olfactory dysfunction was significantly associated with younger age ( P = .002), female ( P = .011), and hyperlipidemia ( P = .012). Gustatory dysfunction was significantly associated with fever ( P = .019) and cough ( P = .039). Conclusion: Olfactory and gustatory dysfunction is a pertinent manifestation of COVID-19. Most of the affected patients achieve rapid and complete recovery. Sudden onset of olfactory and gustatory dysfunction should be recognized as a major symptom of COVID-19 as we implore to contain this pandemic.

scholarly journals Hematological parameters of Hoplias malabaricus (Characiformes: Erythrinidae) parasitized by Monogenea in lagoons in Pirassununga, Brazil

2013 ◽  
Vol 22 (4) ◽  
pp. 457-462 ◽  
Author(s):  
Lincoln Lima Corrêa ◽  
Letícia Cucolo Karling ◽  
Ricardo Massato Takemoto ◽  
Paulo Sérgio Ceccarelli ◽  
Marlene Tiduko Ueta
Keyword(s):  
Condition Factor ◽  
Hematological Parameters ◽  
Hemoglobin Concentration ◽  
Good Health ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Hoplias Malabaricus ◽  
Relative Condition Factor ◽  
The Mean

Hematology is an important pathological and diagnostic tool. This paper describes the hematological parameters of 76 specimens of Hoplias malabaricus infested with monogenean parasites, which were collected from two lagoons. The hematological parameters included: erythrocyte count (Er), microhematocrit (Hct), hemoglobin (Hb), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood parameters showed no significant changes associated with the infestation. Water temperature was found to be negatively correlated with MCV and Hct, with values of rs= −0.52, p<0.0001 and rs= −0.48, p<0.0001, respectively. The mean Relative Condition Factor was Kn=1.01, indicating good health conditions of the fish in these lagoons. No correlation was found between the monogenean infestation and the blood parameters, or between the environmental factors and the monogeneans.

scholarly journals Focal choroidal excavation complicated with choroidal neovascularization in young and middle aged patients

2018 ◽  
Author(s):  
Wenyi Tang ◽  
Ting Zhang ◽  
Qinmeng Shu ◽  
Chunhui Jiang ◽  
Qing Chang ◽  
...  
Keyword(s):  
Choroidal Neovascularization ◽  
Retinal Thickness ◽  
Young Patients ◽  
Central Retinal Thickness ◽  
Middle Aged ◽  
Aged Patients ◽  
Focal Choroidal Excavation ◽  
Anti Vegf ◽  
Mean Width ◽  
The Mean

Abstract Background To investigate the clinical and optical coherence tomography (OCT) features of focal choroidal excavation (FCE) complicated with choroidal neovascularization (CNV) in young and middle aged patients. Methods We performed a retrospective review of 26 patients with FCE accompanied by CNV. All patients underwent a complete ophthalmic examination. We analyzed the clinical characteristics of patients, focusing on the spectral-domain OCT features. All patients received intravitreal injection of anti-vascular endothelial growth factor (anti-VEGF) agents. And we assessed the changes of central retinal thickness and best-corrected visual acuity (BCVA) after anti-VEGF therapy. Results The mean age of 26 patients was 35.5 ± 7.3 years (range, 21 - 48 years). Of the 26 FCE lesions, 11 were located subfoveally, 6 were parafoveal, and 9 were extrafoveal. The mean FCE depth was 129.8 ± 50.3 μm, and the mean width was 901.3 ± 306.0 μm. The FCE depth was correlated positively with the width, but not correlated with age or refractive error. CNV was located within the excavation (19 eyes) or adjacent to the excavation (7 eyes). After anti-VEGF therapy, the central retinal thickness was significantly reduced and the BCVA was significantly improved. In the absorption process of subretinal fluid, we found that the fluid in the excavations needed to be absorbed at the last. A small amount of residual fluid could still be seen in a few deep excavations even after a long-term follow-up. Conclusions FCE may be an important reason to cause CNV. Especially in young patients with idiopathic CNV, we should pay attention to the use of OCT to check the presence of FCE. Anti-VEGF therapy is generally effective for CNV associated with FCE.

scholarly journals Imaging of abdominal complications of COVID-19 infection

BJR|Open ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 20200052
Author(s):  
Damiano Caruso ◽  
Marta Zerunian ◽  
Francesco Pucciarelli ◽  
Elena Lucertini ◽  
Benedetta Bracci ◽  
...  
Keyword(s):  
Laboratory Tests ◽  
Gastrointestinal Symptoms ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Abdominal Ct ◽  
Radiological Features ◽  
Abdominal Symptoms ◽  
Polymerase Chain ◽  
Loss Of Appetite ◽  
Symptoms And Signs

Coronavirus disease 2019 (COVID-19) is a respiratory syndrome caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) first described in Wuhan, Hubei Province, China in the last months of 2019 and then declared as a pandemic. Typical symptoms are represented by fever, cough, dyspnea and fatigue, but SARS-CoV-2 infection can also cause gastrointestinal symptoms (vomiting, diarrhoea, abdominal pain, loss of appetite) or be totally asymptomatic. As reported in literature, many patients with COVID-19 pneumonia had a secondary abdominal involvement (bowel, pancreas, gallbladder, spleen, liver, kidneys), confirmed by laboratory tests and also by radiological features. Usually the diagnosis of COVID-19 is suspected and then confirmed by real-time reverse-transcription-polymerase chain reaction (RT-PCR), after the examination of the lung bases of patients, admitted to the emergency department with abdominal symptoms and signs, who underwent abdominal-CT. The aim of this review is to describe the typical and atypical abdominal imaging findings in patients with SARS-CoV-2 infection reported since now in literature.

scholarly journals Sociodemographic and Hematologic Profile of Breast Cancer Patients Attending a Tertiary Care Teaching Hospital in Ethiopia: Case Control Study

2020 ◽  
Author(s):  
Fatuma Hassen Dejene ◽  
Fikre Enqusilassie Gashe ◽  
Ahmed Ali Ahmed ◽  
Adamu Addise Nuramo ◽  
Girma Taye aweke ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Hematological Parameters ◽  
Descriptive Analysis ◽  
Tertiary Care ◽  
Mean Value ◽  
Diagnosis And Treatment ◽  
Breast Cancer Patients ◽  
The Mean ◽  
Hematologic Profile ◽  
Control Study

Abstract Background: Breast cancer is among the leading causes of cancer death in women. Different socio-demographic factors have impact on early diagnosis and treatment of breast cancer. Hematological abnormalities are also some of the conditions to be monitored during diagnosis and treatment of breast cancer.Objective: To describe socio demographic and hematologic profile of patients with breast cancer at Tikur Anbessa Specialized Hospital.Methods: Case control study was conducted from May 2018 to June 2019. A total of 230 cases and 230 controls participated in the study. Descriptive analysis was made to assess the socio demographic characteristics. Blood samples for hematological parameters were analyzed, using SYSMEXKX21N hematology analyzer. Independent student t- test was performed to compare the mean hematological parameters of cases and controls. Results: The mean age was 42.8 + 12.1 and 39.3 + 11.1 years for cases and controls, respectively. One hundred (43%) of cases and 34 (14.8%) of controls were illiterate. Moreover, 175 (76.1%) of cases and 155 (67.4%) of controls were married. The mean value of Hemoglobin, Red Blood cell, Packed Cell Volume (13.1 + 1.6g/dl, 4.6 + 0.54/1012/L , 38.7 + 4.5 %, respectively) of cases were significantly lower than the controls (14.0 + 1.3g/dl, 4.8 + 0.47 / 1012/L, 40.5 + 3.5% respectively). The mean platelet and total WBC count were 323.4+ 108.1 x 109/L and 7.1 + 2.8 x 109/L for cases and 282.0 +70.0 x 109/L, and 7.1 + 2.4 x 109/L for controls respectively. In addition, 20.4% of cases and 5.6 % of controls were anemic based on their hemoglobin value.Conclusion and recommendations: The majority of breast cancer cases were less than 40 years of age and illiterate. Most of the RBC parameters of cases were significantly lower than the controls. Therefore, attention should be given to those with the designated hematologic abnormalities.

Human Epidermal Growth Factor Receptor 2 Assessment in a Case-Control Study: Comparison of Fluorescence In Situ Hybridization and Quantitative Reverse Transcription Polymerase Chain Reaction Performed by Central Laboratories

2010 ◽  
Vol 28 (28) ◽  
pp. 4300-4306 ◽  
Author(s):  
Frederick L. Baehner ◽  
Ninah Achacoso ◽  
Tara Maddala ◽  
Steve Shak ◽  
Charles P. Quesenberry ◽  
...  
Keyword(s):  
Growth Factor Receptor ◽  
Oncotype Dx ◽  
Her2 Status ◽  
Rt Pcr ◽  
Her2 Positive ◽  
Polysomy 17 ◽  
Epidermal Growth ◽  
Polymerase Chain ◽  
Control Study

Purpose The optimal method to assess human epidermal growth factor receptor 2 (HER2) status remains highly controversial. Before reporting patient HER2 results, American Society of Clinical Oncology (ASCO)/College of American Pathologists (CAP) guidelines mandate that laboratories demonstrate ≥ 95% concordance to another approved laboratory or methodology. Here, we compare central laboratory HER2 assessed by fluorescence in situ hybridization (FISH) and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) using Oncotype DX in lymph node–negative, chemotherapy-untreated patients from a large Kaiser Permanente case-control study. Patients and Methods Breast cancer specimens from the Kaiser–Genomic Health study were examined. Central FISH assessment of HER2 amplification and polysomy 17 was conducted by PhenoPath Laboratories (ratios > 2.2, 1.8 to 2.2, and < 1.8 define HER2 positive, HER2 equivocal, and HER2 negative, respectively). HER2 expression by RT-PCR was conducted using Oncotype DX by Genomic Health (normalized expression units ≥ 11.5, 10.7 to < 11.5, and < 10.7 define HER2 positive, HER2 equivocal, and HER2 negative, respectively). Concordance analyses followed ASCO/CAP guidelines. Results HER2 concordance by central FISH and central RT-PCR was 97% (95% CI, 96% to 99%). Twelve percent (67 of 568 patients) and 11% (60 of 568 patients) of patients were HER2 positive by RT-PCR and FISH, respectively. HER2-positive patients had increased odds of dying from breast cancer compared with HER2-negative patients. Polysomy 17 was demonstrated in 12.5% of all patients and 33% of FISH-positive patients. Nineteen of 20 FISH-positive patients with polysomy 17 were also RT-PCR HER2 positive. Although not statistically significantly different, HER2-positive/polysomy 17 patients tended to have the worst prognosis, followed by HER2-positive/eusomic, HER2-negative/polysomy 17, and HER2-negative/eusomic patients. Conclusion There is a high degree of concordance between central FISH and quantitative RT-PCR using Oncotype DX for HER2 status, and the assay warrants additional study in a trastuzumab-treated population.

scholarly journals Hematological abnormalities and comorbidities are associated with COVID-19 severity among hospitalized patients: Experience from Bangladesh

PLoS ONE ◽  
2021 ◽  
Vol 16 (7) ◽  
pp. e0255379
Author(s):  
Md. Ashrafur Rahman ◽  
Yeasna Shanjana ◽  
Md. Ismail Tushar ◽  
Tarif Mahmud ◽  
Ghazi Muhammad Sayedur Rahman ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Treatment Protocol ◽  
Blood Parameters ◽  
Rt Pcr ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Hematological Abnormalities ◽  
Positive Correlations ◽  
Patients Experience ◽  
D Dimer

Background The hematological abnormalities are assumed to be involved in the disease progression of COVID-19. However, the actual associations between specific blood parameters and COVID-19 are not well understood. Here we aimed to assess the correlations between hematological parameters and the severity of COVID-19. Methods We included COVID-19 patients who were admitted to Evercare Hospital Ltd, Dhaka, Bangladesh, between November 10, 2020, to April 12, 2021, with a confirmed case of RT-PCR test. We recorded demographic information, clinical data, and routine hematological examination results of all COVID-19 patients. We performed statistical analyses and interpretation of data to compare severe COVID-19 patients (SCP) and non-severe COVID-19 patients (NSCP). Results The age and BMI of the admitted COVID-19 patients were 48.79±8.53 years and 25.82±3.75 kg/m2. This study included a total of 306 hospitalized COVID-19 patients. Among them, NSCP and SCP were 198 and 108, respectively. And we recorded 12 deaths from SCP. We observed the alterations of several hematological parameters between SCP and NSCP. Among them, we noticed the increased levels of C-reactive protein (CRP), d-dimer, and ferritin showed good indicative value to evaluate the severity of COVID-19. Also, there were positive correlations among these parameters. Moreover, we found correlations between the outcomes of COVID-19 patients with patient’s demographics and comorbid diseases. Conclusion Based on our results, CRP, d-dimer, and ferritin levels at admission to hospitals represent simple assessment factors for COVID-19 severity and the treatment decisions at the hospital setup. These blood parameters could serve as indicators for the prognosis and severity of COVID-19. Therefore, our study findings might help to develop a treatment protocol for COVID-19 patients at the hospital setup.

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