scholarly journals Fibroblast Growth Factor 9 Correlation with Lymphatic and Vascular Invasion in Colorectal Cancer

2021 ◽  
Vol 14 (2) ◽  
Author(s):  
Leili Rejali ◽  
Seyed Yoosef Seyedna ◽  
Hamid Asadzadeh Aghdaei ◽  
Ehsan Nazemalhosseini Mojarad ◽  
Mehrdad Hashemi
Keyword(s):  
Colorectal Cancer ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Vascular Invasion ◽  
Prognostic Biomarker ◽  
Mrna Level ◽  
Rna Extraction ◽  
Fibroblast Growth ◽  
Fresh Tissue ◽  
Tissue Samples

Background: Fibroblast growth factor 9 (FGF9) or Glia activating factor (GAF) is categorized in the paracrine class of the FGF family, which is involved in various cancer development and progressions. Interestingly, the invasion role of FGF9 in colorectal cancer (CRC) was not clarified up to now. Objectives: In the present investigation, the lymphatic and vascular invasion characteristic of FGF9 was figured out in fresh frozen (FF) tissue samples and paired Formalin-fixed, paraffin-embedded (FFPE) tissues. Methods: The present invasion study according to FGF9 expression evaluation was performed on 80 cancerous resected fresh tissues and 40 paired paraffined block specimens parallel with 80 adjacent non-tumoral tissue samples. RNA extraction and cDNA synthesis were performed; qRT-PCR at mRNA level was applied. FGF9 expression correlation with clinical parameters was defined by the Mann-Whitney U-test. ROC curve and Kaplan-Meier analyses were designed to show the value of prognostic biomarker of FGF9. Results: Accordingly, 52% of fresh tissue samples and 51% of FFPE specimens were upregulated in comparison with corresponding normal tissues. A significant correlation was seen between FGF9 expression level and tumor stage (P < 0.0017, P < 0.03), lymph node metastasis (P < 0.001, P < 0.047), and vascular invasion (P < 0.004, P < 0.047) in fresh tissue samples and paraffined blocks, respectively. ROC was created to distinguish stage I and II from III and IV in FF and FFPE samples, respectively (P < 0.002, P < 0.031). Likewise, the AUC evaluation in both fresh and paraffined samples was similar. The overall survival was lower in 3 years of follow-up in patients with CRC with overexpression of FGF9 (P < 0.02). Conclusions: Altogether, it can be deduced that lymphatic and vascular invasion correlated with FGF9 upregulation since FGF9 can be used as an effective prognostic biomarker according to pathologic results even in paraffined block samples or FF tissue specimens in CRC.

scholarly journals Clinical Significance of Fibroblast Growth Factor (FGF) Expression in Colorectal Cancer

2014 ◽  
Vol 99 (5) ◽  
pp. 493-499 ◽  
Author(s):  
Norie Jibiki ◽  
Noboru Saito ◽  
Shingo Kameoka ◽  
Makio Kobayashi
Keyword(s):  
Colorectal Cancer ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Cancer Patients ◽  
Clinical Significance ◽  
Stage Iv ◽  
Serum Levels ◽  
Lymphatic Invasion ◽  
Fibroblast Growth ◽  
Clinicopathological Factors

Abstract In this study, we serologically and pathologically examined the clinical significance of fibroblast growth factor (FGF) expression in patients with colorectal cancer. Serum basic FGF (bFGF) levels in 92 surgical colorectal cancer patients and 31 controls were measured, and the relationship between those levels and clinicopathological factors were examined. Immunohistochemical study was also conducted on specimens from 51 cancer patients, and the association between bFGF staining and serum levels were investigated. An examination of clinicopathological factors revealed significant differences in bFGF levels between stage 0-IIIb and stage IV cancers (P = 0.013). Lymphatic invasion was one factor that differed significantly. Patients with a tumor 30 mm or smaller had a bFGF level of 7.65 ± 1.11 pg/ml while patients with a tumor 31 mm or larger had a bFGF level of 8.53 ± 3.22 pg/ml; significant differences in these bFGF levels were noted (P &lt; 0.05). Patients with a tumor that had no lymphatic invasion (ly0) had a bFGF level of 7.25 ± 0.66 pg/ml, those with a tumor that had minimal lymphatic invasion (ly1) had a bFGF level of 7.99 ± 1.68 pg/ml, and those with a tumor that had moderate lymphatic invasion (ly2) had a bFGF level of 9.17 ± 4.23 pg/ml. bFGF levels differed significantly for tumors with no/minimal lymphatic invasion (ly0-ly1) and those with moderate lymphatic invasion (ly2) (P &lt; 0.0001). Serological examination of bFGF levels during the proliferation of colorectal cancer revealed that moderate lymphatic invasion can be readily distinguished.

Fibroblast Growth Factor Receptor-2 IIIc as a Novel Molecular Target in Colorectal Cancer

2013 ◽  
Vol 10 (1) ◽  
pp. 20-26
Author(s):  
Yoko Matsuda ◽  
Seiichi Shinji ◽  
Hisashi Yoshimura ◽  
Zenya Naito ◽  
Toshiyuki Ishiwata
Keyword(s):  
Colorectal Cancer ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Molecular Target ◽  
Fibroblast Growth

Postnatal Visual Deprivation in Rats Regulates Several Retinal Genes and Proteins, Including Differentiation-Associated Fibroblast Growth Factor-2

2014 ◽  
Vol 37 (1) ◽  
pp. 14-28 ◽  
Author(s):  
Verena Prokosch-Willing ◽  
Melissa Meyer zu Hoerste ◽  
Sonja Mertsch ◽  
Tobias Stupp ◽  
Solon Thanos
Keyword(s):  
Growth Factor ◽  
Ganglion Cell ◽  
Fibroblast Growth Factor ◽  
Visual Experience ◽  
Mrna Level ◽  
Visual Deprivation ◽  
Fibroblast Growth Factor 2 ◽  
Retinal Cell ◽  
Fibroblast Growth ◽  
Retinal Differentiation

Little is known about the retinal cellular basis of amblyopia, which is a developmental disease characterized by impaired visual acuity. This study examined the retinal transcripts associated with experimentally induced unilateral amblyopia in rats. Surgical tarsorrhaphy of the eyelids on one side was performed in pups prior to eye opening at postnatal day 14, thereby preventing any visual experience. This condition was maintained for over 2 months, after which electroretinograms (ERGs) were recorded, the retinal ganglion cell (RGC) arrangement and number were determined using neuroanatomical tracing, the retinal transcripts were studied using microarray analysis, regulated mRNAs were confirmed with quantitative reverse-transcriptase PCR, and proteins were stained using Western blotting and immunohistochemistry. An attenuated ERG was found in eyes that were deprived of visual experience. Retrograde neuroanatomical staining disclosed a larger number of RGCs within the retina on the visually deprived side compared to the non-deprived, control side, and a multilayered distribution of RGCs. At the retinomic level, several transcripts associated with retinal differentiation, such as fibroblast growth factor 2 (FGF-2), were either up- or downregulated. Most of the transcripts could be verified at the mRNA level. To unravel the role of a differentiation-associated protein, we tested FGF-2 in dissociated postnatal retinal cell cultures and found that FGF-2 is a potent factor triggering ganglion cell differentiation. The data suggest that visual experience shapes the postnatal retinal differentiation, whereas visual deprivation induces changes at the functional, cellular and molecular levels within the retina.

Ethanol induced increase of fibroblast growth factor 2 mRNA content in emotiogenic brain structures of rats

2020 ◽  
Vol 66 (5) ◽  
pp. 419-422
Author(s):  
M.I. Airapetov ◽  
S.O. Eresco ◽  
A.A. Lebedev ◽  
E.R. Bychkov ◽  
P.D. Shabanov
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Dopaminergic Neurons ◽  
Mrna Level ◽  
Brain Structures ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Mrna Content ◽  
The Brain

We studied the effects of acute, subacute, and chronic alcohol treatment of rats on the content of fibroblast growth factor 2 (FGF2) mRNA in various brain structures. Results suggest a possible role of FGF2 in the functioning of dopaminergic neurons in the midbrain. In our experiment, ethanol treatment of rats was accompanied by an increase in the FGF2 mRNA level in the emotiogenic structures of the brain. This effect was blocked by pretreatment of animals with chlorpromazine. This suggests FGF2 involvement in the mechanisms of alcohol dependence and can be considered as a possible diagnostic and therapeutic target in alcoholism.

Fibroblast growth factor receptor 4 as a potential prognostic and therapeutic marker in colorectal cancer

Biomarkers ◽  
2014 ◽  
Vol 19 (1) ◽  
pp. 81-85 ◽  
Author(s):  
Chen-Sheng Li ◽  
Shu-Xiang Zhang ◽  
Hong-Jun Liu ◽  
Yu-Long Shi ◽  
Le-Ping Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fibroblast Growth Factor Receptor ◽  
Growth Factor Receptor ◽  
Fibroblast Growth

Stimulation of Tetrahydrobiopterin Synthesis by Basic Fibroblast Growth Factor in Vascular Endothelial Cells

Pteridines ◽  
2003 ◽  
Vol 14 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Shunichi Shimizu ◽  
Yoshiyuki Miyasaka ◽  
Shinichiro Yamamoto ◽  
Masakazu Ishii ◽  
Yuji Kiuchi
Keyword(s):  
Endothelial Cells ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
De Novo ◽  
Mrna Level ◽  
Mrna Levels ◽  
Dependent Manner ◽  
Fibroblast Growth ◽  
Concentration Dependent Manner

Abstract The purpose of this study was to examine whether basic fibroblast growth factor (bFGF) stimulates tetrahydrobiopterin (BH4) synthesis in mouse brain microvascular endothelial cells. BH4 content was determined by oxidation under acidic conditions as biopterin and analysed with reversed-phase high Performance liquid chromatography. Measurement of the mRNA level of QTP-cyclohydrolase I (GTPCH), which is the rate-limiting enzyme of the de novo pathway of BH4 synthesis. The addition of bFGF to endothelial cells increased the BH4 content and GTPCH mRNA levels in an incubation period- and a concentration-dependent manner. 2,4-Diamino-6- hydroxypyrimidine, an inhibitor of GTPCH, strongly reduced the bFGF-induced increase in BH4 content. These findings suggest that bFGF stimulates BH4 synthesis via a de novo pathway with the induction of GTPCH.

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