scholarly journals Evaluation of Antimicrobial Activity of Some Medicinal Plants on Human Standard Bacteria and Candida albicans

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Bahman Fazeli-Nasab ◽  
Ahmad Farid Rahmani ◽  
Moharram Valizadeh ◽  
Hamide Khajeh ◽  
Maryam Beigomi
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Medicinal Plants ◽  
Bacillus Cereus ◽  
Plant Extracts ◽  
Hypericum Perforatum ◽  
Taxus Baccata ◽  
Hypericum Perforatum L

Background: Continuous and indiscriminate use of chemical drugs causes resistance to microorganisms, which in turn weakens the effect of drugs. This adverse event is associated with an increased number of drugs used by patients and the tendency to use compounds with newer and stronger formulations. Furthermore, the essential oils of several plants contain a significant inhibitory effect on pathogenic microorganisms. Objectives: Hence, the current study intended to evaluate the antimicrobial activity of some medicinal plants on some standard human pathogenic bacteria and Candida albicans fungi isolated from women. Methods: Leaves of Cichorium intybus L., Hypericum perforatum L., Lavandula angustifolia, Thymus vulgaris L., and Taxus baccata L. were collected and analyzed in the botanical laboratory of the University of Zabol. Then, the ethanolic extract was prepared using 40 g of dried leaves in 400 cc of ethanol. Standard bacteria and fungi were obtained from the center for genetic and biological resources of Iran. To determine the activity of free radical trapping, diphenylpicryl hydrazyl was used, and then the antimicrobial effects were investigated by diffusion method in Müller-Hinton agar medium using 6 mm paper disks according to the Bauer and Kirby instructions. Statistical calculations were administered using Statistx Ver10. Mean comparisons were performed using the LSD at the 1% level, and Excel was also used to draw the shapes. Results: The diameter of the inhibitory zone of plant extracts against standard bacteria and clinical fungi of Candida albicans at a dilution of 100 ppm was analyzed, which revealed different effects (P < 0.01). Taxus baccata L., with a 15 mm diameter growth zone, showed the highest effect on inhibiting the growth of Bacillus cereus and Pseudomonas aeruginosa. Hypericum perforatum L. with a diameter of 15 mm was found as the most useful plant in inhibiting Shigella dysentery., with a diameter of 10 mm, was the most useful plant in inhibiting the growth of Escherichia coli. The Taxus baccata L., with a maximum growth inhibition zone diameter (20 mm), has been the most effective plant against Candida albicans. Conclusions: Considering the side effects of chemical drugs and antibiotics as well as the significant effect of medicinal plant extracts used in this study, the Taxus baccata L. was the most useful plant on inhibiting Candida albicans, Bacillus cereus, and Pseudomonas aeruginosa. Moreover, Hypericum perforatum L. was found as the most useful plant to control the growth of Escherichia coli.

scholarly journals Investigation of the Antimicrobial Activity of Rhaponticum (Rhaponticum Carthamoides D.C. Iljin) and Shrubby Cinquefoil (Potentilla Fruticosa L.)

Medicina ◽  
2011 ◽  
Vol 47 (3) ◽  
pp. 24 ◽  
Author(s):  
Vilma Jurkštienė ◽  
Alvydas Pavilonis ◽  
Daiva Garšvienė ◽  
Algirdas Juozulynas ◽  
Laimutė Samsonienė ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Bacillus Cereus ◽  
Potentilla Fruticosa ◽  
Rhaponticum Carthamoides

The aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. Material and Methods. Ethanol extract from the leaves of rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) was produced at the Department of Food Technology, Kaunas University of Technology. The antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035 and fungi Candida albicans ATCC 60193). The minimum inhibitory concentration (MIC) of the examined preparations was determined. Results. Both studied preparations – rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) – demonstrated similar antimicrobial activity. The highest sensitivity to the studied preparations was observed in microbes with eukaryotic cell structure: Candida albicans, which is a fungus, and a spore-forming prokaryotic bacterium, Bacillus cereus. The highest resistance was observed in Escherichia coli and Klebsiella pneumoniae. Conclusions. The studied preparations – viscous extracts of rhaponticum and shrubby cinquefoil – are substances with antimicrobial activity against gram-positive (Staphylococcus aureus and Enterococcus faecalis) and gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) bacteria, spore-forming bacteria (Bacillus subtilis and Bacillus cereus), and fungi (Candida albicans).

scholarly journals Antimicrobial activity of soft and purified propolis extracts

Medicina ◽  
2008 ◽  
Vol 44 (12) ◽  
pp. 977 ◽  
Author(s):  
Alvydas Pavilonis ◽  
Algirdas Baranauskas ◽  
Ligita Puidokaitė ◽  
Žaneta Maželienė ◽  
Arūnas Savickas ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Phenolic Compounds ◽  
Klebsiella Pneumoniae ◽  
Gram Negative ◽  
Propolis Extract

Objective. To evaluate the antimicrobial activity of soft and purified propolis extracts. Study object and methods. Antimicrobial activity of soft and purified propolis extracts was determined with reference cultures of Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035, and fungus Candida albicans ATCC 60193. Microbiological tests were performed under aseptic conditions. Minimum inhibitory concentration (MIC) – the highest dilution of preparation (the lowest concentration of preparation) that suppresses growth of reference microorganisms – was determined. Results. Concentration of phenolic compounds in soft propolis extract that possesses antimicrobial activity against gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis) is 0.587±0.054 mg and 0.587±0.054–0.394±0.022 mg (P>0.05) and in purified propolis extract – 0.427±0.044 mg and 0.256±0.02 mg (P>0.05). Klebsiella pneumoniae is most resistant to soft propolis extract when the concentration of phenolic compounds is 1.119± 0.152 mg and to purified propolis extract when the concentration of phenolic compounds is 1.013±0.189 mg (P>0.05). Spore-forming Bacillus subtilis bacteria are more sensitive to soft and purified propolis extracts when the concentration of phenolic compounds is 0.134±0.002 mg and 0.075±0.025 mg, respectively, and Bacillus cereus – when the concentration is 0.394±0.022 mg and 0.256±0.02 mg (P>0.05). Sensitivity of fungus Candida albicans to soft and purified propolis extracts is the same as Bacillus subtilis. Encapsulated bacterium Klebsiella pneumoniae is most resistant to antimicrobial action of soft and purified propolis extracts as compared with gram-positive Staphylococcus aureus and Enterococcus faecalis bacteria (P<0.05), gram-negative Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis (P<0.05), sporeforming Bacillus subtilis and Bacillus cereus bacteria (P<0.05), and fungus Candida albicans (P<0.05). There is no statistically significant difference between antimicrobial effect of soft propolis extract and purified propolis extract on gram-positive bacteria, gram-negative bacteria, spore-forming bacteria, encapsulated bacteria, and Candida fungus. Conclusions. Soft and purified propolis extracts possess antimicrobial activity. They could be recommended as natural preservatives in the manufacture of pharmaceutical products.

scholarly journals Antimicrobial activity of bioactive component from flower of linum capitatum kit

2004 ◽  
Vol 3 (1) ◽  
pp. 73-77 ◽  
Author(s):  
Slavica Ilic ◽  
Sandra Konstantinovic ◽  
Zoran Todorovic
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Bioactive Components ◽  
Bioactive Component ◽  
Bacillus Subtilus ◽  
Simplex Virus

Different extracts containing bioactive components and etheric oil of the flowers of Linum capitation kit. (Linacea) of Serbian origin were tested for an Antimicrobial activity against four bacteria (Staphylococcus aureus Escherichia coli, Bacillus subtilus, Pseudomonas aeruginosa), one mold (Aspergillus niger) and one yeast (Candida albicans). The isolated Flavonoids were also tested against Staphylococcus aureus, Escherichia coli Bacillus anhtracis, Pseudomonas aeruginosa, Aspergillus niger, Candida albicans and Herpes simplex virus type.

scholarly journals Investigation of Antimicrobial Activity of Medicinal Plant Extracts on Bacillus cereus Isolated from Soil

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Bahman Fazeli-Nasab ◽  
Laleh Shahraki-Mojahed ◽  
Mohammad Amir Hassanzadeh ◽  
Fatemeh Bidarnamani
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Growth Inhibition ◽  
Bacillus Cereus ◽  
Plant Extracts ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Taxus Baccata ◽  
Medicinal Plant Extracts ◽  
Effect On Growth

Background: Because many plant extracts have a significant inhibitory effect on pathogenic microorganisms, so we aimed to evaluate the antioxidant and antimicrobial activity of some plant extracts on Bacillus cereus isolated from soil. Methods: The chicory (Cichorium intybus L.), hyacinth (Hypericum perforatum L.), lavender (Lavandula angustifolia), yew (Taxus baccata), and thyme (Thymus vulgaris L.) plants were collected and identified in the botanical laboratory of the University of Zabol. To prepare the ethanolic extract, 40 g of dried leaves of plants were used in 400 cc of ethanol. Different strains of B. cereus used in this study were isolated from soil and identified by biochemical, bacteriological, and growth tests as well as standard tests. Antimicrobial effects were investigated by diffusion method in Müller Hinton agar medium using 6 mm paper discs according to Bauer and Kirby instructions as well as microdilution. Statistix ver10 software was used for statistical calculations. Mean comparisons were performed using the LSD at the 1% level, and Excel was also used to draw the figures. Results: The diameter of the growth inhibition zone of plant extracts against B. cereus at a dilution of 100 ppm was investigated, and it was found that different extracts had different effects on inhibiting the growth of B. cereus (P < 0.01). LSD test showed that thyme (T. vulgaris L.) had the highest (15 mm) effect on growth inhibition of strains 2 and 3 of B. cereus and the lowest (1 mm) effect on growth inhibition of strain 1 of B. cereus. The lowest MIC and MBC of thyme (T. vulgaris L.) against B. cereus samples were 3.1 and 6.2 ppm, respectively. Conclusions: Considering the side effects of chemical drugs and antibiotics, as well as the potential effect of medicinal plant extracts, especially T. vulgaris L. on B. cereus, it is recommended that T. vulgaris L. may inhibit the growth of B. cereus.

scholarly journals Antibiogram and Phytochemical Analysis of Cinnamon, Clove, and Sichuan Pepper Extracts

2021 ◽  
Vol 9 (1) ◽  
pp. 1-7
Author(s):  
Bibek Adhikari ◽  
Pradeep Kumar Shah ◽  
Roman Karki
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Proteus Mirabilis ◽  
Antimicrobial Properties ◽  
Antimicrobial Activities ◽  
Soxhlet Apparatus ◽  
Medicinal Plant Extracts ◽  
Wide Range

A wide range of medicinal plant extracts has phytochemicals that possess antimicrobial properties and these plants are used to treat several infections. The study aimed to assess the antimicrobial activities of some spices extracts and to evaluate the phytochemicals present in them. The extracts of spices were prepared using Soxhlet apparatus refluxing with methanol and ethanol. The well diffusion technique was implemented for the evaluation of antimicrobial activities of the extracts and the zone of inhibitions was recorded in millimeters. The antimicrobial test was done against five bacterial isolates: Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica serotype Typhi, and Staphylococcus aureus and a fungal isolate: Candida albicans. The extracts were concentrated by Rotary Vacuum Evaporator and a stock solution of 200 mg/mL was prepared by dissolving in 10 % DMSO. Concentrations of 40, 60, 80 and 100 mg/mL extracts were used for antimicrobial activity. The result of this study showed that clove extracts had the highest antimicrobial property against all the test microorganisms. Methanolic extract of clove had the highest inhibitory effect against Proteus mirabilis (24.21±0.15 mm), Pseudomonas aeruginosa (19.78±0.23 mm), and Candida albicans (20.07±0.08 mm) whereas ethanolic extract was effective against Escherichia coli (20.44±0.16 mm), Salmonella Typhi (21.66±0.31 mm) and Candida albicans (21.11±0.09 mm). Cinnamon and pepper extracts, leaving some exceptions, also had antimicrobial properties. The presence of phytochemicals: polyphenols, flavonoids, and tannins are the major components responsible for antimicrobial activity. Thereby, this study successfully demonstrated the possibilities of using spices extracts in the treatment of microbial infections.

scholarly journals LACTOBACILLUS-FERMENTED PLANT JUICE AS A POTENTIAL INGREDIENT IN COSMETICS: FORMULATION AND ASSESSMENT OF NATURAL MOUTHWASH

2016 ◽  
Vol 9 (9) ◽  
pp. 52 ◽  
Author(s):  
Sasithorn Sirilun ◽  
Bhagavathi Sundaram Sivamaruthi ◽  
Naphatsorn Kumar ◽  
Periyanaina Kesika ◽  
Sartjin Peerajan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Clinical Trials ◽  
Medicinal Plants ◽  
Microbial Contamination ◽  
Oral Care ◽  
Peppermint Oil ◽  
Kaempferia Parviflora ◽  
Group A

ABSTRACTObjective: Oral care cosmetics are essential for all populations and are systematically used to treat oral problems. The chemicals free natural cosmeticsare the choice of many people. Thus, this study was aimed to formulate and to assess the natural mouthwash (MW) solution from LactobacillusfermentedThai medicinal plants juice.Methods: The selected (betel, green tea, clove, black galingale, mangosteen, and noni) plant juices were subjected to Lactobacillus plantarum mediatedfermentation. The fermented plant juices (FPJ) were formulated into MW solution with different concentrations of peppermint oil. MW formulationswere assessed for physical appearance, stability, and anti-microbial activities.Results: About 2% of peppermint oil in FPJ was found as organoleptically optimum. The pH and refractive indexes of the MWs were not affectedduring storage and stability assessments. All the FPJ-MWs formulations showed antimicrobial activity against Group A Staphylococcus, and other oralpathogens - Escherichia coli, Streptococcus aureus, and Pseudomonas aeruginosa. Moreover, black galingale, mangosteen, and noni based MW formulasalso exhibited anti-candida activity. The MW made from fermented black galingale (Kaempferia parviflora) juice was the most potent antimicrobialformulation with excellent physical stability.Conclusion: The study concluded that fermented plant-herbal juices can be used as natural MW recipe with 2% of peppermint oil to improve theflavor and aroma. The formulations were stable, free of microbial contamination, and also exhibited antimicrobial activity. Further extended stabilitystudy and clinical trials are necessary to develop a commercial FPJ-based natural MW recipe.Keywords: Fermentation, Formulation, Lactobacillus, Mouthwash.

Activité antimicrobienne de l’huile essentielle de galles du Pistachier de l’Atlas (Pistacia atlantica Desf.) en Algérie

2019 ◽  
Author(s):  
I. Sifi ◽  
M. Yousfi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Salmonella Typhi ◽  
Huiles Essentielles ◽  
Activité Antimicrobienne ◽  
Pistacia Atlantica

L’activité antimicrobienne des huiles essentielles de galles de Pistacia atlantica récoltées dans trois régions (Aïn-Oussara, Laghouat et Kheneg) a été évaluée sur sept souches pathogènes (Escherichia coli, Bacillus cereus, Pseudomonas aeruginosa, Staphylococcus aureus, MRSA+, Salmonella typhi et Candida albicans), en utilisant les méthodes de diffusion sur gélose, de microdilution et celle de microatmosphère (seulement pour Candida albicans). Les huiles essentielles ont révélé un effet antimicrobien remarquable contre les micro-organismes testés, avec des valeurs de CMI et de CMB variant de 0,13 à 7,99 μl/ml et de 0,25 à 7,99 μl/ml, respectivement. Une activité importante a été observée pour les échantillons de la station Kheneg contre l’espèce Candida albicans avec une zone d’inhibition de 68 mm (50 μl). Ces résultats suggèrent que les huiles essentielles testées pour leur activité antimicrobienne peuvent être classées comme bactéricides.

scholarly journals Nickel (II) and Cobalt (II) Complexes of Some Amino Acid-based Surfactants with Antimicrobial Activity

2019 ◽  
Vol 70 (2) ◽  
pp. 361-364
Author(s):  
Dana Varasteanu ◽  
Irina Chican ◽  
Sanda Maria Doncea ◽  
Iuliana Raut ◽  
Mariana Calin ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Amino Acid ◽  
Complex Formation ◽  
Bacillus Cereus ◽  
Aspergillus Flavus ◽  
Antimicrobial Agents ◽  
Cobalt Complexes ◽  
Bacteria And Fungi

Nickel and cobalt complexes of lauroyl-glycine, lauroyl-glycylglycine and 1,12-dodecandioyl-diglycylglycine were synthesized. The FTIR spectroscopy confirmed the complex formation. The antimicrobial activity of synthesized nickel and cobalt complexes was investigated against several bacteria and fungi: Bacillus cereus, Pseudomonas aeruginosa, Candida albicans, and Aspergillus flavus. Generally, the nickel and cobalt complexes of the investigated surfactants showed no antimicrobial activity on Pseudomonas aeruginosa, a moderate one on Bacillus cereus and Aspergillus flavus. The most susceptible microorganism was Candida albicans, which is considered one of most common nosocomial infections. The results suggest the potential application of tested compounds as antimicrobial agents against targeted pathogens.

scholarly journals IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

2017 ◽  
Vol 9 (3) ◽  
pp. 75
Author(s):  
Rajanikanth Garapati ◽  
N. Ramesh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Candida Albicans ◽  
Bacillus Cereus ◽  
Proteus Vulgaris ◽  
Methanol Extracts ◽  
Tract Infections

Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.

The Antimicrobial Activity of Extracts of the Lichen Cetraria aculeata and Its Protolichesterinic Acid Constituent

2003 ◽  
Vol 58 (11-12) ◽  
pp. 850-854 ◽  
Author(s):  
Ayşen Özdemir Türk ◽  
Meral Yılmaz ◽  
Merih Kıvanç ◽  
Hayrettin Türk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Listeria Monocytogenes ◽  
Bacillus Cereus ◽  
Aeromonas Hydrophila ◽  
Proteus Vulgaris ◽  
Ethanol Extracts

Abstract In this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen Cetraria aculeata has been investigated. The extracts were tested against twelve bacteria and eight fungi and found active against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, Proteus vulgaris, Streptococcus faecalis, Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes. No antimicrobial activity against the fungi was detected. It was determined that only one substance in the extracts has antimicrobial activity and it was characterized as protolichesterinic acid. The MICs of the extracts and protolichesterinic acid were also determined.

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