scholarly journals Evaluation of Detection Sensitivity and Pre-enrichment Efficacy of [TA10] Pathogenic Bacterial Multiplex PCR Detection System Kit in Fruit and Vegetable Food Samples

2011 ◽  
Vol 28 (4) ◽  
pp. 219-225
Author(s):  
Susumu KAWASAKI ◽  
Bizhen ZOU ◽  
Toyohiko NAMBA ◽  
Kazuhide ARIMA ◽  
Isao KIUCHI ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Detection System ◽  
Food Samples ◽  
Pcr Detection ◽  
Detection Sensitivity ◽  
Fruit And Vegetable ◽  
Vegetable Food

A Multiplex PCR Assay Mediated by Universal Primers for the Detection of Adulterated Meat in Mutton

2019 ◽  
Vol 82 (2) ◽  
pp. 325-330 ◽  
Author(s):  
WANWAN LIU ◽  
XIAONAN WANG ◽  
JING TAO ◽  
BANGSHENG XI ◽  
MAN XUE ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Detection System ◽  
Meat Products ◽  
Pcr Primers ◽  
Rapid Identification ◽  
Food Samples ◽  
Detection Sensitivity ◽  
Universal Primers ◽  
Multiplex Pcr Assay ◽  
Multiple Pcr

ABSTRACT This study aimed to establish a multiplex PCR detection system mediated by “universal primers,” which would be able to determine whether mutton meat contained nonmutton ingredients from rats, foxes, and ducks. Based on the sequence variation of specific mitochondrial genes, nine different multiplex PCR primers were designed, and four kinds of meat products were rapidly identified by electrophoresis using an optimized multiplex PCR system based on the molecular weight differences of the amplified products. Multiplex PCR applications optimized for meat food source from food samples for testing was used to verify the accuracy of the identification method. The results showed that the primers in multiple PCR system mediated by universal primers could be used for the rapid identification of rat, fox, duck, and sheep meat in mutton products, and the detection sensitivity could reach 0.05 ng/μL. The identification of food samples validated the practical value of this method. Therefore, a multiplex PCR system mediated by universal primers was established, which can be used to quickly identify the origin of animal ingredients from rats, foxes, and ducks in mutton products.

Cultural Relevance of a Fruit and Vegetable Food Frequency Questionnaire

2005 ◽  
Vol 66 (4) ◽  
pp. 231-236 ◽  
Author(s):  
Judy Paisley ◽  
Marlene Greenberg ◽  
Jess Haines
Keyword(s):  
Food Frequency Questionnaire ◽  
Dietary Assessment ◽  
Cultural Relevance ◽  
Assessment Tools ◽  
Comparative Approach ◽  
Fruit And Vegetable ◽  
Food Frequency ◽  
Research And Practice ◽  
Vegetable Food ◽  
Nutrition Research

Purpose: Canada’s multicultural population poses challenges for culturally competent nutrition research and practice. In this qualitative study, the cultural relevance of a widely used semiquantitative fruit and vegetable food frequency questionnaire (FFQ) was examined among convenience samples of adults from Toronto’s Cantonese-, Mandarin-, Portuguese-, and Vietnamesespeaking communities. Methods: Eighty-nine participants were recruited through community-based organizations, programs, and advertisements to participate in semi-structured interviews moderated in their native language. Data from the interviews were translated into English and transcribed for analysis using the constant comparative approach. Results: Four main themes emerged from the analysis: the cultural relevance of the foods listed on the FFQ, words with multiple meanings, the need for culturally appropriate portionsize prompts, and the telephone survey as a Western concept. Conclusions: This research highlights the importance of investing resources to develop culturally relevant dietary assessment tools that ensure dietary assessment accuracy and, more important, reduce ethnocentric biases in food and nutrition research and practice. The transferability of findings must be established through further research.

Multiplex PCR Detection of Enterotoxin Genes in Aeromonas spp. from Suspect Food Samples in Northern Taiwan

2008 ◽  
Vol 71 (10) ◽  
pp. 2094-2099 ◽  
Author(s):  
YU-CHANG CHANG ◽  
JAN-YI WANG ◽  
AMMAIYAPPAN SELVAM ◽  
SHU-CHEN KAO ◽  
SHANG-SHYNG YANG ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Diarrheal Disease ◽  
Simultaneous Detection ◽  
Food Poisoning ◽  
Food Samples ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Enterotoxin Genes ◽  
Causative Agents ◽  
Northern Taiwan

Aeromonads possess an array of virulence factors and are causative agents of a number of human infections. Among them, genes of one cytotoxic (Act) and two cytotonic (Alt, Ast) enterotoxins are implicated in a human diarrheal disease. A rapid, specific, simultaneous detection of these enterotoxin genes in suspected food poisoning samples is not yet reported. Hence, a multiplex PCR assay was designed to amplify the cytotoxic (act), heat-labile cytotonic (alt), and heat-stable cytotonic (ast) enterotoxin genes of aeromonads. The PCR assay was tested with 133 Aeromonas spp. isolated from suspect food poisoning samples and retail samples of poultry and fish from wet markets in and around Taipei, Northern Taiwan. The Aeromonas spp. isolates were divided into six genotypes based on absence or presence of one or more enterotoxin genes. Of these 133 isolates, Aeromonas caviae (52.5%) and Aeromonas hydrophila (43.4%) were the most frequently isolated species from food poisoning samples and retail samples, respectively. Among the species, A. hydrophila had a significantly higher proportion for harboring three enterotoxin genes than had the others, whereas Aeromonas encheleia, considered a nonpathogen, was found harboring three enterotoxin genes. The multiplex PCR assays are rapid and specific, and provide a useful tool for the detection and genotyping of enterotoxin genes of aeromonads.

Multiplex PCR detection of the antibiotic resistance genes in Staphylococcus aureus strains isolated from auricular infections

2008 ◽  
Vol 53 (4) ◽  
pp. 357-362 ◽  
Author(s):  
T. Zmantar ◽  
K. Chaieb ◽  
F. Ben Abdallah ◽  
A. Ben Kahla-Nakbi ◽  
A. Ben Hassen ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Multiplex Pcr ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Pcr Detection

scholarly journals Comprehensive two-dimensional ion chromatography (2D-IC) coupled to a post-column photochemical fluorescence detection system for determination of neonicotinoids (imidacloprid and clothianidin) in food samples

RSC Advances ◽  
2018 ◽  
Vol 8 (17) ◽  
pp. 9277-9286 ◽  
Author(s):  
Nadeem Muhammad ◽  
Fenglian Wang ◽  
Qamar Subhani ◽  
Qiming Zhao ◽  
Muhammad Abdul Qadir ◽  
...  
Keyword(s):  
Ion Chromatography ◽  
Fluorescence Detection ◽  
Detection System ◽  
Food Samples ◽  
Two Dimensional ◽  
Chromatographic Separations ◽  
Fluorescence Determination ◽  
Fluorescence Detection System

A 2D-IC system was successfully fabricated for clean isocratic chromatographic separations and sensitive post column UV induced fluorescence determination of two NNIs in six complex food samples.

scholarly journals Ocurrence of Staphylococcus aureus and multiplex pcr detection of classic enterotoxin genes in cheese and meat products

2009 ◽  
Vol 40 (1) ◽  
pp. 145-148 ◽  
Author(s):  
Marcia Regina Pelisser ◽  
Cátia Silene Klein ◽  
Kelen Regina Ascoli ◽  
Thaís Regina Zotti ◽  
Ana Carolina Maisonnave Arisi
Keyword(s):  
Staphylococcus Aureus ◽  
Multiplex Pcr ◽  
Meat Products ◽  
Pcr Detection ◽  
Enterotoxin Genes

Threshold Radioactivity for Bulk Food Samples by Gamma Spectroscopy

1965 ◽  
Vol 48 (1) ◽  
pp. 1-5
Author(s):  
Harry M Yakabe ◽  
Hiram Neilson
Keyword(s):  
Gamma Ray ◽  
Gamma Spectroscopy ◽  
Rapid Determination ◽  
Detection System ◽  
Fission Products ◽  
Food Samples ◽  
Family Of Curves ◽  
Lower Limits ◽  
True Activity

Abstract In the surveillance of bulk food produce by gamma ray spectroscopy for fission products, the activities of the commonly observed radionuclides are frequently in the magnitude of background inherent to the detection system. The problems of determining whether the sample is in fact contaminated, the lower limits of detecting the radionuclides, and the effect of compton smear on the lower limits are discussed. The discussions are based on the modified spectrum stripping method for quantitative analysis of gamma ray spectrum for the following radioisotopes: Cs-137, Zr-95/Nb-95, and K-40. A family of curves are shown for rapid determination of the minimum detectable true activity (AII) of Cs-137.

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