scholarly journals A ‘universal’ type II chaperonin PCR detection system for the investigation of Archaea in complex microbial communities

2011 ◽  
Vol 6 (2) ◽  
pp. 430-439 ◽  
Author(s):  
Bonnie Chaban ◽  
Janet E Hill
Keyword(s):  
Microbial Communities ◽  
Detection System ◽  
Pcr Detection ◽  
Type Ii

scholarly journals A Novel Pretreatment-Free Duplex Chamber Digital PCR Detection System for the Absolute Quantitation of GMO Samples

2016 ◽  
Vol 17 (3) ◽  
pp. 402 ◽  
Author(s):  
Pengyu Zhu ◽  
Chenguang Wang ◽  
Kunlun Huang ◽  
Yunbo Luo ◽  
Wentao Xu
Keyword(s):  
Detection System ◽  
Digital Pcr ◽  
Pcr Detection ◽  
Absolute Quantitation ◽  
The Absolute

scholarly journals Type II photosynthetic reaction center genes of avocado (Persea americana Mill.) bark microbial communities are dominated by aerobic anoxygenic Alphaproteobacteria

2020 ◽  
Author(s):  
Eneas Aguirre-von-Wobeser
Keyword(s):  
Microbial Communities ◽  
Reaction Center ◽  
Photosynthetic Reaction Center ◽  
Persea Americana ◽  
Algorithm Analysis ◽  
Tree Bark ◽  
Oxygenic Photosynthesis ◽  
Type Ii ◽  
Additional Energy ◽  
Anoxygenic Photosynthesis

AbstractThe tree bark environment is an important microbial habitat distributed worldwide on thrillions of trees. However, the microbial communities of tree bark are largely unknown, with most studies on plant aerial surfaces focused on the leaves. Recently, we presented a metagenomic study of bark microbial communities from avocado. In these communities, oxygenic and anoxygenic photosynthesis genes were very abundant, especially when compared to rhizospheric soil from the same trees. In this work, Evolutionary Placement Algorithm analysis was performed on metagenomic reads orthologous to the PufLM gene cluster, encoding for the bacterial type II photosynthetic reaction center. These photosynthetic genes were found affiliated to different groups of bacteria, mostly aerobic anoxygenic photosynthetic Alphaproteobacteria, including Sphingomonas, Methylobacterium and several Rhodospirillales. These results suggest that anoxygenic photosynthesis in avocado bark microbial communities functions primarily as additional energy source for heterotrophic growth. Together with our previous results, showing a large abundance of cyanobacteria in these communities, a picture emerges of the tree holobiont, where light penetrating the trees canopies and reaching the inner stems, including the trunk, is probably utilized by cyanobacteria for oxygenic photosynthesis, and the far-red light aids the growth of aerobic anoxygenic photosynthetic bacteria.

scholarly journals High-throughput HTLV-1 proviral DNA detection system using a nucleic acid extraction robot and real-time PCR detection.

2001 ◽  
Vol 47 (3) ◽  
pp. 378-383 ◽  
Author(s):  
Chieko Matsumoto ◽  
Rieko Shiozawa ◽  
Shigeki Mitsunaga ◽  
Akiko Ichikawa ◽  
Rika Ishiwatari ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
High Throughput ◽  
Real Time Pcr ◽  
Detection System ◽  
Dna Detection ◽  
Pcr Detection ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Proviral Dna

scholarly journals Multiplex and On-site PCR detection of swine diseases based on the microfluidic chip system

2020 ◽  
Author(s):  
Yan Jiang ◽  
Shan Jiang ◽  
Yue Wu ◽  
Bin Zhou ◽  
Kaiming Wang ◽  
...  
Keyword(s):  
High Throughput ◽  
Microfluidic Chip ◽  
Detection System ◽  
Sampling Site ◽  
High Sensitivity ◽  
Pcr Detection ◽  
Clinical Samples ◽  
Pathogenic Microorganisms ◽  
Real Time Quantitative Pcr ◽  
Inspection And Quarantine

Abstract Background： At present, the process of inspection and quarantine starts with sampling at the customs port, continues with transporting the samples to the central laboratory for inspection experiments, and ends with the inspected results being fed back to the port. This process had the risks of degradation of biological samples and generation of pathogenic microorganisms and did not meet the rapid on-site detection demand because it took a rather long time. Therefore, it is urgently needed to develop a rapid and high-throughput detection assay of pathogenic microorganisms at the customs port. The aim of this study was to develop a microfluidic chip to rapidly detect swine pathogenic microorganisms with high-throughput and higher accuracy. Moreover, this chip will decrease the risk of spreading infection during transportation.Results: A series of experiments were performed to establish a microfluidic chip. The resulting data showed that the positive nucleic acid of four swine viruses were detected by using a portable and rapid microfluidic PCR system, which could achieve a on-site real-time quantitative PCR detection. Furthermore, the detection results of eight clinical samples were obtained within an hour. The detection limit of this microfluidic PCR detection system was as low as 1 copies/μL. The results showed that the high sensitivity and specificity of this chip system in disease detection played an important role in customs inspection and quarantine during customs clearance.Conclusion: The microfluidic PCR detection system established in this study could meet the requirement for rapid detection of samples at the customs port. This chip could avoid the risky process of transporting the samples from the sampling site to the testing lab, and drastically reduce the inspection cycle. Moreover, it would enable parallel inspections on one chip, which greatly raised the efficiency of inspection.

Surfactant protein D: subcellular localization in nonciliated bronchiolar epithelial cells

1992 ◽  
Vol 263 (1) ◽  
pp. L60-L66 ◽  
Author(s):  
E. Crouch ◽  
D. Parghi ◽  
S. F. Kuan ◽  
A. Persson
Keyword(s):  
Epithelial Cells ◽  
Subcellular Localization ◽  
Detection System ◽  
Secretory Granules ◽  
Surfactant Protein ◽  
Clara Cell ◽  
Surfactant Protein D ◽  
Small Airways ◽  
Secretory Product ◽  
Type Ii

Surfactant protein D (SP-D, CP4) is a collagenous surfactant-associated carbohydrate binding protein that was initially characterized as a biosynthetic product of type II pneumocytes. Immunoperoxidase studies of formaldehyde solution-fixed and paraffin-embedded rat lung demonstrated staining for SP-D in the cytoplasm of a subpopulation of bronchiolar epithelial cells as well as type II cells. Accordingly, immunogold-labeling techniques were used to further examine the cellular distribution and subcellular localization of SP-D in the small airways. Lung tissues were fixed with 0.5% glutaraldehyde-3% paraformaldehyde and embedded in LR White resin. Sections were reacted with affinity purified polyclonal antibodies to SP-D, and sites of antibody binding were demonstrated using a biotinylated secondary antibody-streptavidin-gold detection system. Anti-SP-D selectively decorated secretory compartments of nonciliated bronchiolar cells (Clara cells) with strong and specific labeling of apical electron-dense secretory granules. Almost all of the granules in nonciliated columnar cells were labeled; however, labeling was typically nonuniform, with preferential decoration of the periphery of the granule. The largest numbers of immunoreactive epithelial cells were observed in the distal membranous bronchioles, with progressively smaller numbers of cells in more proximal bronchioles. There was no detectable labeling of cells lining the large cartilagenous airways or trachea. These studies provide evidence that SP-D is a secretory product of nonciliated bronchiolar cells. We suggest that Clara cell-derived SP-D is a component of bronchiolar lining material, consistent with our hypothesis that SP-D contributes to surfactant metabolism and/or host defense within small airways.

scholarly journals A Microfluidic Paper-Based Analytical Device for Type-II Pyrethroid Targets in an Environmental Water Sample

Sensors ◽  
2020 ◽  
Vol 20 (15) ◽  
pp. 4107 ◽  
Author(s):  
Sumate Pengpumkiat ◽  
Jintana Nammoonnoy ◽  
Watcharaporn Wongsakoonkan ◽  
Pajaree Konthonbut ◽  
Pornpimol Kongtip
Keyword(s):  
Water Sample ◽  
Detection System ◽  
Low Cost ◽  
Colorimetric Detection ◽  
Environmental Water ◽  
Type Ii ◽  
Environmental Water Sample ◽  
Detection Approach ◽  
Hydrophobic Barrier ◽  
Analytical Device

A detection method for type-II pyrethroids in an environmental water sample using a microfluidic paper-based analytical device (µPAD) is reported here. The detection approach is based on the formation of cyanide from the hydrolysis of type-II pyrethroids and the colorimetric detection of cyanide on a layer-based µPAD. Parafilm and inexpensive laminating pouches were used to create a hydrophobic barrier for the assay on the µPAD. This detection approach was selective to type-II pyrethroids in water for which an environmental water sample was tested. The calibration curves for cypermethrin, deltamethrin, cyhalothrin, and fenvalerate ranged from 2 to 40 µg/mL without sample preconcentration. The lower concentrations of type-II pyrethroids can be assessed by including a preconcentration step prior to the detection on a µPAD. This detection system provides an alternative platform for fast, semiquantitative testing for pesticide contamination in environmental surface water by allowing for portability, low reagent/sample consumption, and low-cost testing.

P123 HPA typing with LinkSe¯q™, a real-time PCR detection system

2016 ◽  
Vol 77 ◽  
pp. 127
Author(s):  
Zachary Antovich ◽  
Thierry Viard ◽  
Roland Russnak ◽  
Queenie Ko ◽  
Komal Singh ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Detection System ◽  
Pcr Detection
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