scholarly journals Molecular detection and characterization of Merkle Cell Polyoma virus in Lymphomas among Sudanese patients

2020 ◽  
pp. 47-51
Author(s):  
Mohammed A IBRAHİM ◽  
Khalid A ENAN ◽  
Abdel Rahim M EL HUSSEİN ◽  
Mohamed O. MUSTAFA ◽  
Bashir SALİM ◽  
...  
Keyword(s):  
Molecular Detection ◽  
Polyoma Virus

Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines

2021 ◽  
Vol 199 ◽  
pp. 106383
Author(s):  
Innina R. Mananguit ◽  
Nest Dale F. Bartolome ◽  
Gabriel Alexis S.P. Tubalinal ◽  
Claro N. Mingala
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
The Philippines

Transformation of hamster embryo cells by chymotrypsin-treated and untreated polyoma virus: characterization of transformants

1985 ◽  
Vol 31 (4) ◽  
pp. 356-360
Author(s):  
Vera Chlumecky ◽  
Donald C. Stranks ◽  
John S. Colter
Keyword(s):  
Common Species ◽  
Soft Agar ◽  
T Antigen ◽  
Polyoma Virus ◽  
Large T Antigen ◽  
T Antigens ◽  
Hamster Embryo Cells ◽  
Embryo Cells

The ability of chymotrypsin-treated (chymo+) and untreated (chymo−) polyoma virus to transform cultured hamster embryo fibroblasts was examined. The data show that exposure to this protease reduces the ability of the virus to transform non-permissive cells to essentially the same extent as it reduces its ability to replicate in permissive cells. Twenty-five lines of transformed cells were established from colonies growing in soft agar, and after 20 in vitro passages, cells of all lines were characterized with respect to their ability to form colonies in soft agar and their tumorigenicity in hamsters. While the studies showed that there are striking differences among the lines with respect to colony-forming ability, and real, though less striking differences in tumorigenicity, they failed to reveal any obvious differences between the groups of cell lines transformed by chymo− and chymo+ polyoma virus. Of 13 lines examined, all were found to express both middle and small polyoma T antigens, none express significant levels of large T antigen, and 11 express some form of what is probably a truncated large T antigen, the most common species having a molecular weight of 67 000.

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 277-282 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
M. T. Rahman
Keyword(s):  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Selective Media ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of S. aureus isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

scholarly journals First molecular detection and genetic characterization of Coxiella burnetii in Zambian dogs and rodents

2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Simbarashe Chitanga ◽  
Edgar Simulundu ◽  
Martin C. Simuunza ◽  
Katendi Changula ◽  
Yongjin Qiu ◽  
...  
Keyword(s):  
Coxiella Burnetii ◽  
Molecular Detection ◽  
Genetic Characterization
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