Molecular detection and characterization of pathogenic Leptospira species in bats (Chiroptera) roosting in human habitats in Nigeria, West Africa

2021 ◽  
Author(s):  
Joshua Kamani ◽  
Shimon Harrus ◽  
Reuben A. Ocholi ◽  
Irene I. Yague ◽  
Patrick G. Nyango ◽  
...  
Keyword(s):  
West Africa ◽  
Molecular Detection ◽  
Pathogenic Leptospira ◽  
Leptospira Species

scholarly journals Characterization of the ompL1 gene of pathogenic Leptospira species in China and cross-immunogenicity of the OmpL1 protein

2008 ◽  
Vol 8 (1) ◽  
pp. 223 ◽  
Author(s):  
Haiyan Dong ◽  
Ye Hu ◽  
Feng Xue ◽  
Dexter Sun ◽  
David M Ojcius ◽  
...  
Keyword(s):  
Pathogenic Leptospira ◽  
Leptospira Species

scholarly journals Genetic Characterization of Pathogenic Leptospira Species by DNA Hybridization

1992 ◽  
Vol 42 (2) ◽  
pp. 215-219 ◽  
Author(s):  
P. Ramadass ◽  
B. D. W. Jarvis ◽  
R. J. Corner ◽  
D. Penny ◽  
R. B. Marshall
Keyword(s):  
Dna Hybridization ◽  
Genetic Characterization ◽  
Pathogenic Leptospira ◽  
Leptospira Species

scholarly journals Structural and Enzymatic Characterization of a cAMP-Dependent Diguanylate Cyclase from Pathogenic Leptospira Species

2017 ◽  
Vol 429 (15) ◽  
pp. 2337-2352 ◽  
Author(s):  
Fernanda Nogales da Costa Vasconcelos ◽  
Nikolas Koshiyama Maciel ◽  
Denize Cristina Favaro ◽  
Luciana Coutinho de Oliveira ◽  
Angela Silva Barbosa ◽  
...  
Keyword(s):  
Diguanylate Cyclase ◽  
Enzymatic Characterization ◽  
Pathogenic Leptospira ◽  
Leptospira Species

scholarly journals Ethnobotanical characterization of scarlet eggplant (Solanum aethiopicum L.) varieties cultivated in Benin (West Africa)

2021 ◽  
pp. 100173
Author(s):  
Scholastique Aguessy ◽  
Roger Idossou ◽  
Anicet G. Dassou ◽  
Yêyinou Laura Estelle Loko ◽  
Octaviano Igor Yelome ◽  
...  
Keyword(s):  
West Africa ◽  
Solanum Aethiopicum

Molecular detection and genetic characterization of ovine gammaherpesvirus-2 (OvHV-2) in sheep in the Philippines

2021 ◽  
Vol 199 ◽  
pp. 106383
Author(s):  
Innina R. Mananguit ◽  
Nest Dale F. Bartolome ◽  
Gabriel Alexis S.P. Tubalinal ◽  
Claro N. Mingala
Keyword(s):  
Molecular Detection ◽  
Genetic Characterization ◽  
The Philippines

scholarly journals Genomic investigation of a dengue virus outbreak in Thiès, Senegal, in 2018

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Amy Gaye ◽  
Tolla Ndiaye ◽  
Mouhamad Sy ◽  
Awa B. Deme ◽  
Alphonse B. Thiaw ◽  
...  
Keyword(s):  
West Africa ◽  
Dengue Virus ◽  
Clinical Signs ◽  
Health Concern ◽  
Public Health Concern ◽  
Local Circulation ◽  
Viral Genomes ◽  
Genomic Characterization ◽  
The City

AbstractDengue virus is a major and rapidly growing public health concern in tropic and subtropic regions across the globe. In late 2018, Senegal experienced its largest dengue virus outbreak to date, covering several regions. However, little is known about the genetic diversity of dengue virus (DENV) in Senegal. Here we report complete viral genomes from 17 previously undetected DENV cases from the city of Thiès. In total we identified 19 cases of DENV in a cohort of 198 individuals with fever collected in October and November 2018. We detected 3 co-circulating serotypes; DENV 3 was the most frequent accounting for 11/17 sequences (65%), 4 (23%) were DENV2 and 2 (12%) were DENV1. Sequences were most similar to recent sequences from West Africa, suggesting ongoing local circulation of viral populations; however, detailed inference is limited by the scarcity of available genomic data. We did not find clear associations with reported clinical signs or symptoms, highlighting the importance of testing for diagnosing febrile diseases. Overall, these findings expand the known range of DENV in Senegal, and underscore the need for better genomic characterization of DENV in West Africa.

Detection and characterization of Leptospira spp. in dogs diagnosed with kidney and/or liver disease in Selangor, Malaysia

2021 ◽  
pp. 104063872110245
Author(s):  
Sabri A. Rahman ◽  
Kuan H. Khor ◽  
Siti Khairani-Bejo ◽  
Seng F. Lau ◽  
Mazlina Mazlan ◽  
...  
Keyword(s):  
Liver Disease ◽  
Mortality Rate ◽  
Direct Detection ◽  
Bacterial Disease ◽  
Pathogenic Leptospira ◽  
Wide Range ◽  
Leptospira Spp ◽  
Rrna Sequencing ◽  
The Common

Leptospirosis is a serious bacterial disease that affects both humans and animals. A wide range of symptoms have been described in humans; the disease in dogs is commonly associated with kidney and/or liver disease. In Malaysia, information about the common serovars infecting dogs is limited. Therefore, we investigated the occurrences of leptospirosis in 124 pet dogs diagnosed with kidney and/or liver disease. Blood, urine, abdominal effusion, and/or kidney and liver were collected from the dogs. Based on microscopic agglutination testing, 53 of 124 (42.7%) dogs were seropositive for leptospiral exposure. Sera were frequently positive to serovars Bataviae ( n = 12), Javanica ( n = 10), and Icterohaemorrhagiae ( n = 10). Direct detection using PCR showed that 42 of 124 (33.9%) of the whole blood and 36 of 113 (31.9%) urine samples were positive for pathogenic Leptospira spp. By PCR, 2 of 23 (9.1%) kidney and 2 of 23 (9.1%) liver were positive for pathogenic Leptospira spp. Abdominal effusion from 4 dogs were PCR-positive for pathogenic Leptospira spp. The species detected were L. interrogans, L. borgpetersenii, L. kirschneri, and L. kmetyi by partial 16S rRNA sequencing. We further identified and characterized 11 Leptospira spp. isolates from 8 dogs as serovars Bataviae, Javanica, and Australis. The mortality rate of the Leptospira-infected dogs was high (18 of 53; 34%).

scholarly journals Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates

2009 ◽  
Vol 58 (9) ◽  
pp. 1173-1181 ◽  
Author(s):  
Gustavo M. Cerqueira ◽  
Alan J. A. McBride ◽  
Mathieu Picardeau ◽  
Samuel G. Ribeiro ◽  
Ângela N. Moreira ◽  
...  
Keyword(s):  
Clinical Isolates ◽  
Pathogenic Leptospira ◽  
Reliable Identification ◽  
Specific Pcr ◽  
The Family ◽  
Leptospira Species

The family of leptospiral immunoglobulin-like (lig) genes comprises ligA, ligB and ligC. This study used PCR to demonstrate the presence of lig genes among serovars from a collection of leptospiral strains and clinical isolates. Whilst ligA and ligC appeared to be present in a limited number of pathogenic serovars, the ligB gene was distributed ubiquitously among all pathogenic strains. None of the lig genes were detected among intermediate or saprophytic Leptospira species. It was also shown that, similar to the previously characterized secY gene, a short specific PCR fragment of ligB could be used to correctly identify pathogenic Leptospira species. These findings demonstrate that ligB is widely present among pathogenic strains and may be useful for their reliable identification and classification.

Protein typing of major outer membrane lipoproteins from Chinese pathogenic Leptospira spp. and characterization of their immunogenicity

Vaccine ◽  
2009 ◽  
Vol 28 (1) ◽  
pp. 243-255 ◽  
Author(s):  
Dongjiao Luo ◽  
Feng Xue ◽  
David M. Ojcius ◽  
Jinfang Zhao ◽  
Yafei Mao ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Pathogenic Leptospira ◽  
Leptospira Spp ◽  
Membrane Lipoproteins
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