scholarly journals Synthesis, Characterization of Dichlorofluorescein Silver Nanoparticles (DCF-SNPs) and Their Effect on Seed Germination ofVigna radiata

2021 ◽  
Author(s):  
Bipin Deochand Lade ◽  
Arti Sanjay Shanware ◽  
Ruchika M. Barapatre
Keyword(s):  
Silver Nanoparticles ◽  
Seed Germination ◽  
Detection System ◽  
Average Diameter ◽  
Germination Percentage ◽  
Surface Load ◽  
Microscopy Technique ◽  
Efficient Detection ◽  
The Impact

The main objective of this study was to investigate whether dichlorofluorescein (DCF) is adequate for the formulation of stable dichlorofluorescein-induced silver nanoparticles under the boiling method to analyze their effects on the seed germination of Mung seeds (Vigna radiata). Preliminary dichlorofluoresceine nanoparticles (DCF-SNPs) synthesis evidence by noticing the solution color transformed from a light green color to a dark brown color. The 2.5 ml of dichlorofluoresceine (DCF) solution was found sufficient for the formulation of dichlorofluoresceine induced silver nanoparticles at boiling conditions. Purified dichlorofluoresceine nanoparticles (DCF-SNPs) measure an average diameter of 293 nm where the majority of nanoparticles were around 159 nm in size with the surface load of-9.35 mV zeta potential value. The impact of dichlorofluorescein silver nanoparticles (DCF-SNPs) on the germination percentage of V. radiata has shown that, the 25% concentration of DCF-SNPs is excellent for the growth of Mung seeds (V. radiata). Overall, the dichlorofluorescein silver nanoparticles may be constructive for improving the percentage of seed germination at 25% of its concentration and may also be useful for fluorescent measurement using the confocal microscopy technique. Hence, dichlorofluorescein silver nanoparticles (DCF-SNPs) are proposed as an efficient detection system for nanoparticles in agrochemicals for plants.

scholarly journals Physical characterization of Rhipsalis (Cactaceae) fruits and seeds germination in different temperatures and light regimes

2016 ◽  
Vol 76 (2) ◽  
pp. 367-373 ◽  
Author(s):  
A. B. Lone ◽  
R. C. Colombo ◽  
B. L. G. Andrade ◽  
L. S. A. Takahashi ◽  
R. T. Faria
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Light Regimes ◽  
Total Absence ◽  
Germination Characteristics ◽  
Different Temperatures ◽  
Medium Time ◽  
Seeds Germination

Abstract The germination characteristics of the native cactus species are poorly known, being the temperature and the light the factors that the most interferes in that process. Thus, the objective of the present work was to characterize the fruits and evaluate the influence of the temperature and the light in the seed germination of Rhipsalis floccosa, Rhipsalis pilocarpa and Rhipsalis teres. The tested constant temperatures were 15, 20, 25, 30 and 35 °C and the alternate of 20-30 °C and 25-35 °C in a photoperiod of 10 hours, and with determination of the most appropriate temperature, the germination was tested in light absence. The germination percentage, the index of germination speed and medium time of germination were evaluated. For R. floccosa, the highest germination percentage was at 20 °C. For R. pilocarpa and R. teres, the highest germination percentages occurred in 15 °C and 20 °C. There was correlation to germination percentage between the three species, indicating that they had similar germination behavior. Total absence of germination was verified for the three species in condition of light absence. In conclusion, the temperature of 20 °C is the most suitable for the seed germination of R. floccosa. For the species R. pilocarpa and R. teres, the temperatures of 15 and 20 °C are the most suitable.

scholarly journals Impact of seed size on seeds viability, vigor and storability of Hordeum vulgare (L.)

2018 ◽  
Author(s):  
M. Massimi
Keyword(s):  
Seed Germination ◽  
Seed Size ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Dry Weight ◽  
Germination Percentage ◽  
Seedling Vigor ◽  
Hordeum Vulgare L ◽  
Vigor Index ◽  
The Impact

Seed size may influence seed germination, and seedling vigor. Few investigations are available about the effect of seed size on barley seedling vigor in Jordan. The present study was designed to investigate the impact of seed size on germination percentage, seedling dry weight, seedling vigor index, and germination percentage after accelerated aging in barley. Three seed sizes i.e. large, medium, and small (having diameter of > 2.75 millimeter, 2.5 - 2.75 millimeter, and less than 2.5 millimeter, respectively) were tested in the experiment. Seed quality for different seed size categories was evaluated in the laboratory by measuring seed germination, germination after accelerated aging, as well seedling dry weight and vigor index. Results showed significant differences for large seeds in germination percentages, seedling dry weight and vigor index. It may be concluded that large seed size of barley showed best quality.

MPACT OF ENVIRONMENT TEMPERATURE AND STRATIFICATION ON THE CITRUS AURANTIUM SEED GERMINATION

2021 ◽  
Author(s):  
V. N. Sorokopudov ◽  
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Citrus Aurantium ◽  
Speed Increase ◽  
Maximum Percentage ◽  
Percentage Decrease ◽  
Environment Temperature ◽  
Germination Speed ◽  
The Impact

This study is aimed at investigating the impact of the environment temperature and stratification on the Citrus aurantium seed germination. The results show that the optimum environment temperature for the seed germination is found to be 25°C with the maximum percentage of 90%. The stratification of seeds leads to the seed germination percentage decrease by 69% and germination speed increase by 7 days.

scholarly journals Sugar apple (Annona squamosa L.) seed germination affected by the application of gibberellins

2016 ◽  
Vol 34 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Fabio Ernesto Martínez M. ◽  
Diego Miranda L. ◽  
Stanislav Magnitskiy
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Positive Response ◽  
Germination Rate ◽  
Germination Percentage ◽  
Annona Squamosa ◽  
Germination Time ◽  
Mean Germination Time ◽  
Germinated Seeds

This research sought to establish the response of the germination percentage (PG), synchrony index (E), mean germination time (MGT) and mean germination rate (MGR) of Annona squamosa L. seeds from Apulo (province of Cundinamarca) and Castilla (province of Tolima), Colombia, to treatments with 0, 50, 100, 200, 400, 600, or 800 mg L-1 of gibberellic acid (GA). All of the treatments with GA increased the PG at each point of time of seed incubation. The 600 mg L-1 GA treatment resulted in higher PGs (92.3% at 16 days for Apulo and 95% at 24 days for Castilla) and lower MGTs (8.75 and 5.38 days for Apulo and Castilla, respectively) than those found with the concentration of 0 mg L-1 GA (17.68 and 10.88 days for Apulo and Castilla, respectively). Also, treating the seeds with 600 mg L-1 GA generated higher MGRs (0.18 and 0.12 germinated seeds/day for Castilla and Apulo, respectively) than those obtained with 0 mg L-1 GA (Castilla = 0.09 and Apulo = 0.06 germinated seeds/ day). Likewise, the germination was synchronized with the application of any concentration of GA. The results evidenced a positive response to the GA application, which provided a tool for the characterization of the phenomenon of dormancy in the A. squamosa seeds.

scholarly journals Ion chromatographic analysis of combined industrial effluent and its impact on seed germination of leguminous seeds

2013 ◽  
Vol 14 (3) ◽  
pp. 123-130
Author(s):  
Nishu K. ◽  
Raj Kumar Rampal ◽  
Sajad Iqbal Khan
Keyword(s):  
Seed Germination ◽  
Chromatographic Analysis ◽  
Industrial Effluent ◽  
Germination Percentage ◽  
Germination Index ◽  
Sodium Potassium ◽  
Leguminous Plants ◽  
Industrial Estate ◽  
The Impact ◽  
Leguminous Seeds

The present study has been made to assess the impact of combined industrial effluent from Industrial estate Bari Brahamana Jammu on seed germination of leguminous plants. The study revealed that values of anions fluoride, chloride, bromide, phosphate and sulphate and cations sodium, potassium and calcium o decreased with increase in dilution. In general in most of studied seeds it was observed that the values of seed germination percentage and rate of seed germination index decreased with increasing effluent concentration.

scholarly journals IMPACT OF BULK AND NANO-ZINC OXIDE ON GROWTH OF CICER ARIETRIUM (CHICKPEAS)

2020 ◽  
pp. 1-2
Author(s):  
Shilpa Shilpa ◽  
Sushmita Kushwaha ◽  
Shweta Pathania ◽  
Divya Gupta
Keyword(s):  
Zinc Oxide ◽  
Seed Germination ◽  
Antimicrobial Properties ◽  
Germination Percentage ◽  
Shoot Length ◽  
Zno Nps ◽  
Control Seed ◽  
Nano Zinc Oxide ◽  
Nano Zinc ◽  
The Impact

INTRODUCTION: Zinc oxide nanoparticles (ZnO-NPs) exhibit remarkable optical, physical, and antimicrobial properties and therefore have great potential to enhance agriculture. But the use of these NPs leads to their discharge and accumulation in the environment affecting both plant and animals systems, which emphasizes to study the toxicity of both bulk and NPs. AIM: The present study is aimed at investigating the impact of ZnO-NPs and ZnO bulk particles on chickpeas. METHOD: Two parameters were examined in this study: seed germination percentage and shoot length. Different concentration (250, 500, 1000 and 2000 mg/L) of ZnO-NPs and ZnO bulk particles were prepared in distilled water and used for the treatment in chickpeas. RESULT: The study showed that the use of the ZnO-NPs and ZnO bulk particles both can reduce the seed germination and shoot length under controlled condition in comparison with control seed. With increase in the concentration from 250mg/L>500mg/L >1000mg/L >2000mg/L the shoot length decreases CONCLUSION: It was concluded that the ZnO-NPs affect the physiological expression of plant. It was found to stunt shoot length.

scholarly journals Green synthesis and characterization of silver nanoparticles using Alcea rosea flower extract as a new generation of antimicrobials

2017 ◽  
Vol 23 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Alireza Ebrahiminezhad ◽  
Yahya Barzegar ◽  
Younes Ghasemi ◽  
Aydin Berenjian
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Inhibitory Concentration ◽  
Average Diameter ◽  
Flower Extract ◽  
Escherichia Coli Bacteria ◽  
Alcea Rosea ◽  
New Generation ◽  
Significant Factors

Green synthesis of silver nanoparticles (AgNPs) was developed by treating Ag+ with Alcea rosea flower extract. AgNO3 concentration, flower extract quantity, and reaction temperature were found to be significant factors in the bioreduction reaction. Synthesized AgNPs were almost spherical in shape with an average diameter of 7.2 nm. Fourier transform infrared spectroscopy (FTIR) analysis revealed that oxygen-bearing functional groups in the A. rosea flower extract are responsible for reduction of Ag+. The minimum inhibitory concentration (MIC) of AgNPs against a Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria was determined to be 37.5 ?g/ml.

scholarly journals Leaf Extracts Silver Nanoparticles of Four Medicinally Important Plants: Used as a Green Manure

2020 ◽  
pp. 1-8
Author(s):  
Sk Md Abu Imam Saadi ◽  
Amal Kumar Mondal
Keyword(s):  
Silver Nanoparticles ◽  
Seed Germination ◽  
Germination Rate ◽  
Germination Percentage ◽  
Biomedical Science ◽  
Leaf Extracts ◽  
Plant Samples ◽  
Indigenous Plant ◽  
Average Size

Now a day’s green synthesis of silver nanoparticles (AgNPs) from plants has an important role in biomedical science, drug discovery and also in biological field. In this investigation, we synthesize AgNPs using the aqueous solution of the leaf extract of 4 indigenous plant samples such as –Abroma augusta, Barringtonia acutangula, Dillenia indica & Eupatorium odoratum in room temperature (35±2°C). After that, formation of AgNPs were confirmed by UV-VIS spectrum, the answered (435, 445, 430 & 440 nm), respectively. The average size of the AgNPs also confirmed by Particle size analyser (PAS), the B. acutangular species showed the best quality of silver nanoparticles among the other plant extracts. FTIR analysis showed that five biomolecule groups like phenols, aromatic, alkyne, alkane and alkene were found in among these plant samples. Our findings suggest that the seed germination percentage, relative seed germination rate, relative shoot & root growth and germination index of the tested plant depends upon concentration gradient of synthesize AgNPs. At 0.6 mg/ml concentration, the tested plant samples give the best favorable growth condition.

scholarly journals Alkalinity Showed Limited Effect on Turfgrass Germination under Low to Moderate Salinity

HortScience ◽  
2014 ◽  
Vol 49 (9) ◽  
pp. 1201-1204
Author(s):  
Qi Zhang ◽  
Kevin Rue
Keyword(s):  
Seed Germination ◽  
Festuca Arundinacea ◽  
Cynodon Dactylon ◽  
Poa Pratensis ◽  
Germination Rate ◽  
Creeping Bentgrass ◽  
Kentucky Bluegrass ◽  
Germination Percentage ◽  
Alkaline Conditions ◽  
The Impact

Saline and alkaline conditions often coexist in nature. Unlike salinity that causes osmotic and ionic stresses, alkalinity reflects the impact of high pH on plant growth and development. In this research, seven turfgrass species, tall fescue (Festuca arundinacea Schreb.), kentucky bluegrass (Poa pratensis L.), creeping bentgrass (Agrostis stolonifera L.), perennial ryegrass (Lolium perenne L.), zoysiagrass (Zoysia japonica Steud.), bermudagrass [Cynodon dactylon var. dactylon (L.) Pers.], and alkaligrass [Puccinellia distans (Jacq.) Parl.], were germinated under 10 saline–alkaline conditions [two salinity concentrations (25 and 50 mm) × five alkalinity levels (pH = 7.2, 8.4, 9.1, 10.0, 10.8)] in a controlled environment. Seed germination was evaluated based on final germination percentage and daily germination rate. Alkaligrass and kentucky bluegrass showed the highest and lowest germination under saline conditions, respectively. Limited variations in germination were observed in other species, except bermudagrass, which showed a low germination rate at 50 mm salinity. Alkalinity did not cause a significant effect on seed germination of tested turfgrass species.

Predicting the impact of increasing temperatures on seed germination among populations of Western Australian Banksia (Proteaceae)

2014 ◽  
Vol 24 (3) ◽  
pp. 195-205 ◽  
Author(s):  
Anne Cochrane ◽  
Gemma L. Hoyle ◽  
Colin J. Yates ◽  
Jeff Wood ◽  
Adrienne B. Nicotra
Keyword(s):  
Climate Change ◽  
Seed Germination ◽  
Natural Populations ◽  
Population Level ◽  
Germination Percentage ◽  
Individual Species ◽  
Mean Annual Temperature ◽  
Consistent Manner ◽  
The Impact ◽  
Germination Niche

AbstractTemperature is a significant factor influencing seed germination and for many species temperature-mediated germination cues are vital for plant persistence. Rising temperatures forecast as a result of anthropogenic climate change may have a substantial influence on the population and range dynamics of plant species. Here, we report on the thermal constraints on seed germination in natural populations of four congeneric Banksia species collected from a longitudinal climate gradient in Western Australia. We investigated whether germination niche: (1) varied between species; (2) varied among populations of each species; and (3) varied in a consistent manner reflecting the climatic gradients of seed origin. We hypothesized that species would differ and that populations from warmer sites would have a broader temperature window for germination than populations from cooler sites. Species differed in the breadth of their germination niche, but temperatures that stimulated the most rapid and complete germination were similar across all species. A sharp reduction in germination percentage occurred above the optimum temperature, which coincided with significant delays in germination relative to the optimum. The temperatures causing these declines varied among populations. Across the species, there was a significant correlation between optimum germination temperature and mean annual temperature at seed source; however, there was no relationship at the population level for individual species. These data provide insight into the vulnerability of Banksia species to climate change, with those populations that require lower temperatures for germination, or have narrower optimal ranges for germination, likely to be most vulnerable to a warming climate.

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