scholarly journals Introductory Chapter: Importance of Zebrafish (Danio rerio) as Model Organism in Biomedical Research

2020 ◽  
Author(s):  
Yusuf Bozkurt
Keyword(s):  
Biomedical Research ◽  
Danio Rerio ◽  
Model Organism

scholarly journals The Importance of Zebrafish in Biomedical Research

2013 ◽  
Vol 26 (5) ◽  
pp. 583
Author(s):  
Bárbara Tavares ◽  
Susana Santos Lopes
Keyword(s):  
Biomedical Research ◽  
Drug Screening ◽  
Danio Rerio ◽  
Scientific Literature ◽  
Model Organism ◽  
Toxicity Testing ◽  
Vertebrate Development ◽  
Automated Systems ◽  
Ideal Model ◽  
Research Drug

Introduction: Zebrafish (Danio rerio) is an ideal model organism for the study of vertebrate development. This is due to the large clutches that each couple produces, with up to 200 embryos every 7 days, and to the fact that the embryos and larvae are small, transparent and undergo rapid external development.Material and Methods: Using scientific literature research tools available online and the keywords Zebrafish, biomedical research, human disease, and drug screening, we reviewed original studies and reviews indexed in PubMed.Results: In this review we summarized work conducted with this model for the advancement of our knowledge related to several human diseases. We also focused on the biomedical research being performed in Portugal with the zebrafish model.Discussion: Powerful live imaging and genetic tools are currently available for zebrafish making it a valuable model in biomedicalresearch. The combination of these properties with the optimization of automated systems for drug screening has transformed the zebrafish into a “top model” in biomedical research, drug discovery and toxicity testing. Furthermore, with the optimization of xenografts technology it will be possible to use zebrafish to aide in the choice of the best therapy for each patient.Conclusion: Zebrafish is an excellent model organism in biomedical research, drug development and in clinical therapy.

scholarly journals Organism-Level Tumor Models in Zebrafish Danio rerio

Acta Naturae ◽  
2018 ◽  
Vol 10 (2) ◽  
pp. 24-29
Author(s):  
I. V. Mizgirev ◽  
D. R. Safina ◽  
I. V. Demidyuk ◽  
S. V. Kostrov
Keyword(s):  
Tumor Cell ◽  
Biomedical Research ◽  
Danio Rerio ◽  
Fluorescent Microscopy ◽  
Tumor Formation ◽  
Transcription Analysis ◽  
New Information ◽  
Optically Transparent ◽  
Tumor Studies ◽  
Effective Models

Development and implementation of adequate organism-level models is one of the key elements in biomedical research that focuses on experimental oncology. Over the last decade, studies using Zebrafish (Danio rerio) have gained in popularity in this area of research. This review describes the various approaches that have been used in developing highly effective models for oncological (clinical term, better cancer or tumor) studies based on D. rerio. Priority is given to transplantation models of cancer and their application to optically transparent D. rerio lines, including clonal ones, and utilization tumors of various origins bearing fluorescent labels. The combination of tumor transplantation at organism-level models in transparent clonal D. rerio lines with fluorescent microscopy, FACS-fractionation of tumor cell subsets, and transcription analysis can result in one of the most promising research approaches in providing new information on tumor formation and growth.

Gill membrane remodeling with soft-water acclimation in zebrafish (Danio rerio)

2007 ◽  
Vol 30 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Paul M. Craig ◽  
Chris M. Wood ◽  
Grant B. McClelland
Keyword(s):  
Danio Rerio ◽  
Metal Toxicity ◽  
Model Organism ◽  
Fold Increase ◽  
Soft Water ◽  
Membrane Remodeling ◽  
Protein Levels ◽  
High Degree ◽  
Carbonic Anhydrase 1 ◽  
Mrna Expression Levels

Little is known regarding the ionoregulatory abilities of zebrafish exposed to soft water despite the popularity of this model organism for physiology and aquatic toxicology. We examined genomic and nongenomic changes to gills of zebrafish as they were progressively acclimated from moderately hard freshwater to typical soft water over 7 days and held in soft water for another 7 days. Gills were sampled daily and mRNA expression levels of gill Na+-K+-ATPase (NKA) α1a subunit, epithelium calcium channel (ECaC), carbonic anhydrase-1 and 2 (CA-1, CA-2), Na+/H+ exchanger (NHE-2), V-type proton (H+)-ATPase, and copper transport protein (CTR-1) were quantified by real-time PCR. Changes in enzyme activities of gill NKA were determined and protein levels of NKA and ECaC were quantified by Western blotting. Levels of mRNA for ECaC increased fourfold after day 6, with an associated increase in ECaC protein levels after 1 wk in soft water. CA-1 and CA-2 exhibited a 1.5- and 6-fold increase in gene expression on days 6 and 5, respectively. Likewise, there was a fivefold increase in NHE-2 expression after day 6. Surprisingly, CTR-1 mRNA showed a large transient increase (over threefold) on day 6, while H+-ATPase mRNA did not change. These data demonstrate a high degree of phenotypic plasticity in zebrafish gills exposed to an ion-poor environment. This not only enhances our understanding of ionoregulatory processes in fish but also highlights the need for proper experimental design for studies involving preacclimation to soft water (e.g., metal toxicity).

scholarly journals Effect of β-Cyclodextrin Stabilized Silver Nanoparticles on Gills, Kidney, Liver of Danio rerio

2021 ◽  
Vol 11 (1) ◽  
pp. 2981-2995
Keyword(s):  
Silver Nanoparticles ◽  
Danio Rerio ◽  
Morphological Changes ◽  
Antimicrobial Property ◽  
Research Work ◽  
Model Organism ◽  
Histopathological Study ◽  
Histopathological Studies ◽  
Kidney Liver ◽  
Drug Studies

The silver nanoparticles (AgNP) are applied broadly in medical applications due to their antimicrobial property. However, the toxicity (uptake, translocation, and accumulation) of these AgNPs nanomaterial has not been much explored. Also, cyclodextrin has been used for different pharmaceutical applications due to its various potential properties. Therefore, the toxicity of these AgNPs and cyclodextrin in the model organism such as Danio rerio could be crucial for future nano-drug studies. The main aim of the present research work is to synthesize, characterize biopolymeric silver nanoparticles, and perform histopathological studies of synthesized silver nanoparticles on Danio rerio. The silver nanoparticles of 130nm size have been synthesized successfully using β-Cyclodextrin (β-CD) at room temperature. The various analytical applied to characterized the β-Cyclodextrin (β-CD) reduced silver nanoparticles (β-CD)-SNPs. A histopathological study has been conducted to evaluate the toxicity effect of the synthesized (β-CD)-SNP on the animal model Danio rerio. The (β-CD)-SNPs conc. 30µl/l is affecting and damaged gills and kidney the Danio rerio organs (gills, kidney) exposed after 10 days, yet the liver was found to be healthy. In conclusion, the Danio rerio gills, kidneys, and liver are sensitive to the 130nm-sized (β-CD)-SNPs. The nanoparticles' toxicity depends on concentration; less concentration (30µl/l) accumulates and is absorbed efficiently than the higher concentration (300µl/l). However, no morphological changes were observed on Danio rerio.

scholarly journals Zebrafish (Danio rerio) as a Model Organism

2019 ◽  
Author(s):  
Farmanur Rahman Khan ◽  
Saleh Sulaiman Alhewairini
Keyword(s):  
Danio Rerio ◽  
Model Organism

scholarly journals Assessing the toxicity of Pb- and Sn-based perovskite solar cells in model organism Danio rerio

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Aslihan Babayigit ◽  
Dinh Duy Thanh ◽  
Anitha Ethirajan ◽  
Jean Manca ◽  
Marc Muller ◽  
...  
Keyword(s):  
Solar Cells ◽  
Danio Rerio ◽  
Perovskite Solar Cells ◽  
Model Organism

Pichia pastoris: protein production host and model organism for biomedical research

2013 ◽  
Vol 8 (2) ◽  
pp. 191-208 ◽  
Author(s):  
Brigitte Gasser ◽  
Roland Prielhofer ◽  
Hans Marx ◽  
Michael Maurer ◽  
Justyna Nocon ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Biomedical Research ◽  
Protein Production ◽  
Model Organism ◽  
Production Host

Zebrafish (Danio rerio) as a Model Organism for Dementia

2010 ◽  
pp. 255-269
Author(s):  
Rob Willemsen ◽  
Sandra van’t Padje ◽  
John C. van Swieten ◽  
Ben A. Oostra
Keyword(s):  
Danio Rerio ◽  
Model Organism

scholarly journals Yolk–Water Partitioning of Neutral Organic Compounds in the Model Organism Danio rerio

2020 ◽  
Vol 39 (8) ◽  
pp. 1506-1516
Author(s):  
Nadin Ulrich ◽  
Nicole Schweiger ◽  
Andrea Pfennigsdorff ◽  
Stefan Scholz ◽  
Kai‐Uwe Goss
Keyword(s):  
Organic Compounds ◽  
Danio Rerio ◽  
Model Organism ◽  
Water Partitioning ◽  
Neutral Organic Compounds

scholarly journals Polyethylene glycol (5,000) succinate conjugate of lopinavir and its associated toxicity using Danio rerio as a model organism

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Oluwole Samuel Aremu ◽  
Lebogang Katata-Seru ◽  
Zimbili Mkhize ◽  
Tarryn Lee Botha ◽  
Victor Wepener
Keyword(s):  
Polyethylene Glycol ◽  
Danio Rerio ◽  
Model Organism
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