scholarly journals Staphylococcus aureus in the Meat Supply Chain: Detection Methods, Antimicrobial Resistance, and Virulence Factors

2020 ◽  
Author(s):  
Valeria Velasco ◽  
Mario Quezada-Aguiluz ◽  
Helia Bello-Toledo
Keyword(s):  
Staphylococcus Aureus ◽  
Supply Chain ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Detection Methods

scholarly journals Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil

2020 ◽  
Vol 51 (4) ◽  
pp. 2111-2122
Author(s):  
Verónica K. C. Pérez ◽  
Dircéia A. C. Custódio ◽  
Eduarda M. M. Silva ◽  
Julia de Oliveira ◽  
Alessandro S. Guimarães ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Bovine Mastitis

scholarly journals Complete genome sequencing of Staphylococcus aureus: insights into virulence and antimicrobial resistance

2008 ◽  
Vol 29 (3) ◽  
pp. 115
Author(s):  
Benjamin Howden ◽  
Tim Stinear ◽  
John K Davies
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Genome Sequencing ◽  
Virulence Factors ◽  
Complete Genome ◽  
Bacterial Pathogens ◽  
Complete Genome Sequencing

Staphylococcus aureus remains one of the most important opportunistic bacterial pathogens of humans. It is characterised by the presence of many virulence factors, as well as an ability to rapidly develop or acquire antimicrobial resistance.

scholarly journals Relationship between virulence factors and antimicrobial resistance in Staphylococcus aureus from bovine mastitis

2020 ◽  
Vol 22 ◽  
pp. 792-802 ◽  
Author(s):  
Verónica Karen Castro Pérez ◽  
Geraldo Márcio da Costa ◽  
Alessandro Sá Guimarães ◽  
Marcos Bryan Heinemann ◽  
Andrey Pereira Lage ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Bovine Mastitis

scholarly journals Genetic Diversity and Virulence Factors of S. aureus Isolated from Food, Humans, and Animals

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Roberto Adame-Gómez ◽  
Natividad Castro-Alarcón ◽  
Amalia Vences-Velázquez ◽  
Jeiry Toribio-Jiménez ◽  
Abigail Pérez-Valdespino ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
High Resistance ◽  
Commensal Bacterium ◽  
Gene Coding ◽  
Pcr Rflp ◽  
Multiple Environments

Staphylococcus aureus is a commensal bacterium in humans and animals able to adapt to multiple environments. The aim of this study was to compare the genetic diversity and virulence profiles of strains of S. aureus isolated from food (29 strains), humans (43 strains), and animals (8 strains). 80 lipase-producing strains belonging to a biobank of 360 isolates, identified phenotypically as S. aureus, were selected. Confirmation of the species was made by amplifying the spA gene and 80% (64/80) of the strains were confirmed within this species. The virulence profile of each of the isolates was determined by PCR. The seA gene coding for enterotoxin A was found in 53.1% of the strains, the saK gene, which codes for Staphylokinase, was amplified in 57.8% of the strains, and, finally, the hlB gene coding for β-Hemolysin was amplified in 17.2%. The profile of antimicrobial resistance was determined by the Kirby Bauer method showing that the strains from food presented greater resistance to erythromycin (40.7%) and ciprofloxacin (18.5%) while in strains isolated from humans were to erythromycin (48.4%) and clindamycin (21.2%). Also, in strains from animals, a high resistance to erythromycin was observed (75%). The frequency of MRSA was 12.5% due to the presence of the mec gene and resistance to cefoxitin. Of the total strains, 68.7% were typed by PCR-RFLP of the coa gene using the AluI enzyme; derived from this restriction, 17 profiles were generated. Profile 4 (490 bp, 300 bp) was the most frequent, containing a higher number of strains with a higher number of virulence factors and antimicrobial resistance, which is associated with greater adaptation to different environments. In this study, a wide genetic diversity of strains of S. aureus from different foods, humans, and animals was found. This demonstrates evolution, genetic versatility, and, therefore, the adaptation of this microorganism in different environments.

scholarly journals New update on molecular diversity of clinical Staphylococcus aureus isolates in Iran: Antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing

2021 ◽  
Author(s):  
Mahtab Tabandeh ◽  
Hami Kaboosi ◽  
Mojtaba Taghizadeh Armaki ◽  
Abazar Pournajaf ◽  
Fatemeh Peyravii Ghadikolaii
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Resistance Genes ◽  
Biofilm Formation ◽  
Clonal Selection ◽  
Clinical Samples ◽  
Inducible Resistance ◽  
Antibiotic Susceptibility Test ◽  
Wide Range

Abstract Staphylococcus. aureus is often considered as a potential pathogen and resistant to a wide range of antibiotics. The pathogenicity of this bacterium is due to the presence of multiple virulence factors and ability to form biofilm. SCCmec types I, II and III are mainly attributed to HA-MRSA, while SCCmec types IV and V have usually been reported in CA-MRSA infections. In this study, we performed a cross-sectional study in order to determine the antimicrobial resistance, adhesion and virulence factors, biofilm formation and SCCmec typing of clinical S. aureus isolates in Iran. S. aureus isolate was identified using microbiological standard methods and antibiotic susceptibility test was performed as described by the Clinical and Laboratory Standards Institute (CLSI) guidelines. Inducible resistance phenotype and biofilm formation were determined using D-test and tissue culture plate methods, respectively. Multiplex-PCRs were performed to detect adhesion and virulence factors, antibiotic resistance genes, biofilm formation and SCCmec typing by specific primers. Among 143 clinical samples, 67.8% were identified as MRSA. All isolates were susceptible to vancomycin. The prevalence of cMLSB, iMLSB and MS phenotypes were 61.1%, 22.2% and 14.8%, respectively. The TCP method revealed that 71.3% of isolates were able to form biofilm. The predominant virulence and inducible resistance genes in both MRSA and MSSA isolates were related to sea and ermC respectively. SCCmec type III was the predominant type. Data show the high prevalence rates of virulence elements among S. aureus isolates, especially MRSA strains. This result might be attributed to antibiotic pressure, facilitating clonal selection.

scholarly journals Distribution and Clonal Diversity of Staphylococcus aureus and Other Staphylococci in Surface Waters: Detection of ST425-t742 and ST130-t843 mecC-Positive MRSA Strains

Antibiotics ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1416
Author(s):  
Vanessa Silva ◽  
Eugénia Ferreira ◽  
Vera Manageiro ◽  
Lígia Reis ◽  
María Teresa Tejedor-Junco ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Multidrug Resistance ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
Resistance Genes ◽  
Surface Waters ◽  
Water Samples ◽  
Coagulase Negative Staphylococci ◽  
Spa Typing ◽  
Antimicrobial Resistance Genes

Natural aquatic environments represent one of the most important vehicles of bacterial dissemination. Therefore, we aimed to isolate staphylococci from surface waters and to investigate the presence of antimicrobial resistance genes and virulence factors as well as the genetic lineages of all Staphylococcus aureus isolates. Staphylococci were recovered from water samples collected from 78 surface waters, including rivers, streams, irrigation ditches, dams, lakes, and fountains. The presence of antimicrobial resistance genes and virulence factors was investigated by PCR. Multilocus sequence typing and spa-typing were performed in all S. aureus isolates. From the 78 water samples, 33 S. aureus, one S. pseudintermedius, and 51 coagulase-negative staphylococci (CoNS) were identified. Among the S. aureus isolates, four MRSA were identified, and all harbored the mecC gene. Fourteen S. aureus were susceptible to all antimicrobials tested and the remaining showed resistance to penicillin, erythromycin and/or tetracycline encoded by the blaZ, ermT, msr(A/B), tetL, and vgaA genes. Regarding the clonal lineages, one mecC-MRSA isolate belonged to spa-type t843 and sequence type (ST) 130 and the other three to t742 and ST425. The remaining S. aureus were ascribed 14 spa-types and 17 sequence types. Eleven species of CoNS were isolated: S. sciuri, S. lentus, S. xylosus, S. epidermidis, S. cohnii spp. urealyticus, S. vitulinus, S. caprae, S. carnosus spp. Carnosus, S. equorum, S. simulans, and S. succinus. Thirteen CoNS isolates had a multidrug resistance profile and carried the following genes: mecA, msr(A/B), mph(C), aph(3′)-IIIa, aac(6′)-Ie–aph(2′’)-Ia, dfrA, fusB, catpC221, and tetK. A high diversity of staphylococci was isolated from surface waters including mecCMRSA strains and isolates presenting multidrug-resistance profiles. Studies on the prevalence of antibiotic-resistant staphylococci in surface waters are still very scarce but extremely important to estimate the contribution of the aquatic environment in the spread of these bacteria.

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