scholarly journals The Injectable rhBMP-2-containing Collagen Gel for Tendon Healing in a Rabbit Extra-Articular Bone Tunnel Model

Tendons ◽  
2019 ◽  
Author(s):  
Kwang-Il Lee ◽  
Ju-Woong Jang ◽  
Kwang-Won Lee
Keyword(s):  
Collagen Gel ◽  
Tendon Healing ◽  
Bone Tunnel ◽  
Tunnel Model

Tendon-bone interface healing using an injectable rhBMP-2-containing collagen gel in a rabbit extra-articular bone tunnel model

2015 ◽  
Vol 11 (5) ◽  
pp. 1435-1441 ◽  
Author(s):  
Kwang Won Lee ◽  
Jung Soo Lee ◽  
Ju Woong Jang ◽  
Young Bock Shim ◽  
Kwang-Il Lee
Keyword(s):  
Collagen Gel ◽  
Bone Tunnel ◽  
Bone Interface ◽  
Tunnel Model

Tendon-healing in a bone tunnel. A biomechanical and histological study in the dog.

1993 ◽  
Vol 75 (12) ◽  
pp. 1795-1803 ◽  
Author(s):  
S A Rodeo ◽  
S P Arnoczky ◽  
P A Torzilli ◽  
C Hidaka ◽  
R F Warren
Keyword(s):  
Histological Study ◽  
Tendon Healing ◽  
Bone Tunnel

scholarly journals The effects of growth and differentiation factor 5 on bone marrow stromal cell transplants in an in vitro tendon healing model

2011 ◽  
Vol 36 (4) ◽  
pp. 271-279 ◽  
Author(s):  
M. Hayashi ◽  
C. Zhao ◽  
K.-N. An ◽  
P. C. Amadio
Keyword(s):  
Bone Marrow ◽  
Collagen Gel ◽  
Tendon Healing ◽  
Culture Conditions ◽  
Flexor Digitorum Profundus ◽  
Maximal Strength ◽  
Differentiation Factor ◽  
Cell Transplants ◽  
Flexor Digitorum

The effects of growth differentiation factor-5 (GDF-5) and bone marrow stromal cells (BMSCs) on tendon healing were investigated under in vitro tissue culture conditions. BMSCs and GDF-5 placed in a collagen gel were interpositioned between the cut ends of dog flexor digitorum profundus tendons. The tendons were randomly assigned into four groups: 1) repaired tendon without gel; 2) repaired tendon with BMSC-seeded gel; 3) repaired tendon with GDF-5 gel without cells; and 4) repaired tendon with GDF-5 treated BMSC-seeded gel. At 2 and 4 weeks, the maximal strength of repaired tendons with GDF-5 treated BMSCs-seeded gel was significantly higher than in tendons without gel interposition. However, neither BMSCs nor GDF-5 alone significantly increased the maximal strength of healing tendons at 2 or 4 weeks. These results suggest that the combination of BMSCs and GDF-5 accelerates tendon healing, but either BMSCs or GDF-5 alone are not effective in this model.

Tendon healing in a bone tunnel. Part I

2002 ◽  
Vol 18 (2) ◽  
pp. 113-123 ◽  
Author(s):  
Andreas Weiler ◽  
Ricarda Peine ◽  
Alireza Pashmineh-Azar ◽  
Clemens Abel ◽  
Norbert P. Südkamp ◽  
...  
Keyword(s):  
Tendon Healing ◽  
Bone Tunnel

Augmentation of Tendon Healing in an Intraarticular Bone Tunnel with Use of a Bone Growth Factor

2001 ◽  
Vol 29 (6) ◽  
pp. 689-698 ◽  
Author(s):  
Kyle Anderson ◽  
Aruna M. Seneviratne ◽  
Kazutaka Izawa ◽  
Brent L. Atkinson ◽  
Hollis G. Potter ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Growth ◽  
Cruciate Ligament ◽  
Tendon Healing ◽  
Tendon Graft ◽  
Bone Tunnel ◽  
Resonance Imaging ◽  
Load To Failure ◽  
Anterior Cruciate ◽  
Bone Growth Factor

We hypothesized that an exogenous bone growth factor could augment healing of a tendon graft in a bone tunnel in a rabbit anterior cruciate ligament-reconstruction model. Seventy rabbits underwent bilateral anterior cruciate ligament reconstructions with a semitendinosus tendon graft. One limb received a collagen sponge carrier vehicle containing a mixture of bone-derived proteins while the contralateral limb was treated with either no sponge or a sponge without bone-derived proteins. The reconstruction was evaluated at 2, 4, or 8 weeks with histologic, biomechanical, and magnetic resonance imaging analysis. Histologic analysis demonstrated that specimens treated with bone-derived proteins had a more consistent, dense interface tissue and closer apposition of new bone to the graft, with occasional formation of a fibrocartilaginous interface, when compared with control specimens. The treated specimens had significantly higher load-to-failure rates than did control specimens. Treatment with bone-derived proteins resulted in an average increase in tensile strength of 65%. The treated specimens were stronger than control specimens at each time point, but the difference was greatest at 8 weeks. On the basis of signal characteristics and new bone formation, magnetic resonance imaging was useful for predicting which limb was treated, the site of failure, and the limbs with higher load-to-failure values. This study demonstrates the potential for augmenting tendon healing in an intraarticular bone tunnel using an osteoinductive growth factor.

Tendon Healing in a Bone Tunnel Differs at the Tunnel Entrance versus the Tunnel Exit

2006 ◽  
Vol 34 (11) ◽  
pp. 1790-1800 ◽  
Author(s):  
Scott A. Rodeo ◽  
Sumito Kawamura ◽  
Hyon-Jeong Kim ◽  
Christian Dynybil ◽  
Liang Ying
Keyword(s):  
Tendon Healing ◽  
Bone Tunnel ◽  
Tunnel Entrance

scholarly journals Fibrin Glue-Kartogenin Complex Promotes the Regeneration of the Tendon-Bone Interface in Rotator Cuff Injury

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Jun Zhu ◽  
Jiahua Shao ◽  
Yi Chen ◽  
Guangyi Zhao ◽  
Lexiang Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Rotator Cuff ◽  
Fibrin Glue ◽  
Tendon Graft ◽  
Bone Tunnel ◽  
Rotator Cuff Tendon ◽  
Bone Interface ◽  
Tunnel Model ◽  
Rotator Cuff Injury

Objective. Rotator cuff injury healing is problematic because the tendon-bone junction often forms cicatricial tissues, rather than fibrocartilage, which leads to mechanical impairment and is prone to redamage. Kartogenin (KGN) is a newly discovered small molecule compound which can induce cartilage formation through chondrogenesis of endogenous mesenchymal stem cells. Methods. In this study, we used KGN with fibrin glue (FG) to repair the rotator cuff injury by promoting the formation of fibrocartilage at the tendon to bone interface. Firstly, we assessed the release rate of KGN from the FG-KGN complex and then created a rabbit rotator cuff tendon graft-bone tunnel model. The rabbits received saline, FG-KGN, or FG injections onto the tendon to bone interface after injury. Shoulder tissues were harvested at 6 and 12 weeks, and the sections were stained with HE and Safranin O/Fast green. The samples were assessed by histologic evaluation and biomechanical testing. Synovial mesenchymal stem cells derived from the synovial tissue around the rotator cuff were harvested for western blotting and qRT-PCR analysis. Results. KGN was released rapidly from the FG-KGN complex during first 4 hrs and followed by a slow release until 7 days. The tendon graft-bone interface in the control (saline) group and the FG group was filled with scar tissue, rather than cartilage-like tissue, and only a small number of chondrocytes were found at the adjacent bone surface. In the FG-KGN group, the tendon to bone interface was fully integrated and populated by chondrocytes with proteoglycan deposition, indicating the formation of fibrocartilage-like tissues. At 12 weeks, the maximum tensile strength of the FG-KGN group was significantly higher than that of the FG and control groups ( P < 0.01 ). The RNA expression levels of tendinous genes such as Tenascin C and the chondrogenic gene Sox-9 were substantially elevated in SMSCs treated with the FG-KGN complex compared to the other two groups. Conclusion. These results indicated that fibrin glue is an effective carrier for KGN, allowing for the sustained release of KGN. The FG-KGN complex could effectively promote the regeneration and formation of fibrocartilage tissue of the tendon-bone interface in the rabbit rotator cuff tendon graft-bone tunnel model.

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