scholarly journals Metabolic Engineering of the Model Photoautotrophic Cyanobacterium Synechocystis for Ethanol Production: Optimization Strategies and Challenges

2019 ◽  
Author(s):  
J. Tony Pembroke ◽  
Patricia Armshaw ◽  
Michael P. Ryan
Keyword(s):  
Metabolic Engineering ◽  
Ethanol Production ◽  
Production Optimization

Metabolic engineering of bacteria for ethanol production

1998 ◽  
Vol 58 (2-3) ◽  
pp. 204-214 ◽  
Author(s):  
L. O. Ingram ◽  
P. F. Gomez ◽  
X. Lai ◽  
M. Moniruzzaman ◽  
B. E. Wood ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Ethanol Production

Bio-ethanol production optimization using ACD with ESN critic

2011 ◽  
Author(s):  
V. Iliev ◽  
G. Kostov ◽  
J. Stoycheva ◽  
P. Koprinkova-Hristova ◽  
M. Angelov ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Production Optimization

scholarly journals Improvement of Xylose Uptake and Ethanol Production in Recombinant Saccharomyces cerevisiae through an Inverse Metabolic Engineering Approach

2005 ◽  
Vol 71 (12) ◽  
pp. 8249-8256 ◽  
Author(s):  
Yong-Su Jin ◽  
Hal Alper ◽  
Yea-Tyng Yang ◽  
Gregory Stephanopoulos
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Rate ◽  
Metabolic Engineering ◽  
Ethanol Production ◽  
Pichia Stipitis ◽  
Pentose Phosphate ◽  
Gene Target ◽  
Reading Frame ◽  
Engineering Approach ◽  
Inverse Metabolic Engineering

ABSTRACT We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subculturing enrichment of the transformant library, 16 transformants were identified and confirmed to have a higher growth rate on xylose. Sequencing of the 16 plasmids isolated from these transformants revealed that the majority of the inserts (10 of 16) contained the XYL3 gene, thus confirming the previous finding that XYL3 is the consensus target for increasing xylose assimilation. Following a sequential search for gene targets, we repeated the complementation enrichment process in a XYL1 XYL2 XYL3 background and identified 15 fast-growing transformants, all of which harbored the same plasmid. This plasmid contained an open reading frame (ORF) designated PsTAL1 based on a high level of homology with S. cerevisiae TAL1. To further investigate whether the newly identified PsTAL1 ORF is responsible for the enhanced-growth phenotype, we constructed an expression cassette containing the PsTAL1 ORF under the control of a constitutive promoter and transformed it into an S. cerevisiae recombinant expressing XYL1, XYL2, and XYL3. The resulting recombinant strain exhibited a 100% increase in the growth rate and a 70% increase in ethanol production (0.033 versus 0.019 g ethanol/g cells · h) on xylose compared to the parental strain. Interestingly, overexpression of PsTAL1 did not cause growth inhibition when cells were grown on glucose, unlike overexpression of the ScTAL1 gene. These results suggest that PsTAL1 is a better gene target for engineering of the pentose phosphate pathway in recombinant S. cerevisiae.

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