Metabolic engineering of high yield ethanol production in Thermoanaerobacterium saccharolyticum.

2008 ◽  
Author(s):  
Arthur Josephus Shaw
Keyword(s):  
Metabolic Engineering ◽  
Ethanol Production ◽  
High Yield ◽  
Thermoanaerobacterium Saccharolyticum

scholarly journals Inhibition of Pyruvate Kinase From Thermoanaerobacterium saccharolyticum by IMP Is Independent of the Extra-C Domain

2021 ◽  
Vol 12 ◽  
Author(s):  
Christopher A. Fenton ◽  
Qingling Tang ◽  
Daniel G. Olson ◽  
Marybeth I. Maloney ◽  
Jeffrey L. Bose ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Ethanol Production ◽  
Pyruvate Kinase ◽  
Surprising Result ◽  
Sugar Phosphate ◽  
Allosteric Inhibitors ◽  
Truncated Protein ◽  
First Report ◽  
Thermoanaerobacterium Saccharolyticum

The pyruvate kinase (PYK) isozyme from Thermoanaerobacterium saccharolyticum (TsPYK) has previously been used in metabolic engineering for improved ethanol production. This isozyme belongs to a subclass of PYK isozymes that include an extra C-domain. Like other isozymes that include this extra C-domain, we found that TsPYK is activated by AMP and ribose-5-phosphate (R5P). Our use of sugar-phosphate analogs generated a surprising result in that IMP and GMP are allosteric inhibitors (rather than activators) of TsPYK. We believe this to be the first report of any PYK isozyme being inhibited by IMP and GMP. A truncated protein that lacks the extra C-domain is also inhibited by IMP. A screen of several other bacterial PYK enzymes (include several that have the extra-C domain) indicates that the inhibition by IMP is specific to only a subset of those isozymes.

Metabolic engineering of Geobacillus thermoglucosidasius for high yield ethanol production

2009 ◽  
Vol 11 (6) ◽  
pp. 398-408 ◽  
Author(s):  
R.E. Cripps ◽  
K. Eley ◽  
D.J. Leak ◽  
B. Rudd ◽  
M. Taylor ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Ethanol Production ◽  
High Yield ◽  
Geobacillus Thermoglucosidasius

scholarly journals Metabolic Engineering of Fungal Strains for Conversion of d-Galacturonate to meso-Galactarate

2009 ◽  
Vol 76 (1) ◽  
pp. 169-175 ◽  
Author(s):  
Dominik Mojzita ◽  
Marilyn Wiebe ◽  
Satu Hilditch ◽  
Harry Boer ◽  
Merja Penttilä ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Aspergillus Niger ◽  
Galacturonic Acid ◽  
Raw Material ◽  
High Yield ◽  
Hypocrea Jecorina ◽  
Bacterial Gene ◽  
Fungal Strains ◽  
Gene Coding ◽  
Reductive Pathway

ABSTRACT d-Galacturonic acid can be obtained by hydrolyzing pectin, which is an abundant and low value raw material. By means of metabolic engineering, we constructed fungal strains for the conversion of d-galacturonate to meso-galactarate (mucate). Galactarate has applications in food, cosmetics, and pharmaceuticals and as a platform chemical. In fungi d-galacturonate is catabolized through a reductive pathway with a d-galacturonate reductase as the first enzyme. Deleting the corresponding gene in the fungi Hypocrea jecorina and Aspergillus niger resulted in strains unable to grow on d-galacturonate. The genes of the pathway for d-galacturonate catabolism were upregulated in the presence of d-galacturonate in A. niger, even when the gene for d-galacturonate reductase was deleted, indicating that d-galacturonate itself is an inducer for the pathway. A bacterial gene coding for a d-galacturonate dehydrogenase catalyzing the NAD-dependent oxidation of d-galacturonate to galactarate was introduced to both strains with disrupted d-galacturonate catabolism. Both strains converted d-galacturonate to galactarate. The resulting H. jecorina strain produced galactarate at high yield. The A. niger strain regained the ability to grow on d-galacturonate when the d-galacturonate dehydrogenase was introduced, suggesting that it has a pathway for galactarate catabolism.

scholarly journals The redox-sensing protein Rex modulates ethanol production in Thermoanaerobacterium saccharolyticum

PLoS ONE ◽  
2018 ◽  
Vol 13 (4) ◽  
pp. e0195143 ◽  
Author(s):  
Tianyong Zheng ◽  
Anthony A. Lanahan ◽  
Lee R. Lynd ◽  
Daniel G. Olson
Keyword(s):  
Ethanol Production ◽  
Thermoanaerobacterium Saccharolyticum ◽  
Redox Sensing

scholarly journals Marker Removal System forThermoanaerobacterium saccharolyticumand Development of a Markerless Ethanologen

2011 ◽  
Vol 77 (7) ◽  
pp. 2534-2536 ◽  
Author(s):  
A. Joe Shaw ◽  
Sean F. Covalla ◽  
David A. Hogsett ◽  
Christopher D. Herring
Keyword(s):  
High Yield ◽  
Thermoanaerobacterium Saccharolyticum ◽  
Selective Strategy ◽  
Removal System

ABSTRACTMarker removal strategies were developed forThermoanaerobacterium saccharolyticumto select against thepyrFgene and theptaandackgenes. Thepta- andack-based haloacetate selective strategy was subsequently used to create strain M0355, a markerless ΔldhΔptaΔackstrain that produces ethanol at a high yield.

Non-photosynthetic plastids as hosts for metabolic engineering

2018 ◽  
Vol 62 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Silas Busck Mellor ◽  
James B.Y.H. Behrendorff ◽  
Agnieszka Zygadlo Nielsen ◽  
Poul Erik Jensen ◽  
Mathias Pribil
Keyword(s):  
Metabolic Engineering ◽  
Molecular Mechanisms ◽  
Plant Cells ◽  
High Yield ◽  
Plant Fitness ◽  
Design Strategies ◽  
Plastid Differentiation ◽  
High Level ◽  
Cellular Compartmentalization ◽  
And Storage

Using plants as hosts for production of complex, high-value compounds and therapeutic proteins has gained increasing momentum over the past decade. Recent advances in metabolic engineering techniques using synthetic biology have set the stage for production yields to become economically attractive, but more refined design strategies are required to increase product yields without compromising development and growth of the host system. The ability of plant cells to differentiate into various tissues in combination with a high level of cellular compartmentalization represents so far the most unexploited plant-specific resource. Plant cells contain organelles called plastids that retain their own genome, harbour unique biosynthetic pathways and differentiate into distinct plastid types upon environmental and developmental cues. Chloroplasts, the plastid type hosting the photosynthetic processes in green tissues, have proven to be suitable for high yield protein and bio-compound production. Unfortunately, chloroplast manipulation often affects photosynthetic efficiency and therefore plant fitness. In this respect, plastids of non-photosynthetic tissues, which have focused metabolisms for synthesis and storage of particular classes of compounds, might prove more suitable for engineering the production and storage of non-native metabolites without affecting plant fitness. This review provides the current state of knowledge on the molecular mechanisms involved in plastid differentiation and focuses on non-photosynthetic plastids as alternative biotechnological platforms for metabolic engineering.

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