Fluorescent Markers in Water Treatment

Fading of the diaminobenzidine reaction product in the confocal scanning laser microscope

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100152707 ◽

1989 ◽

Vol 47 ◽

pp. 146-147

Author(s):

JS Deitch ◽

KL Smith ◽

JW Swann ◽

JN Turner

Keyword(s):

Pyramidal Neurons ◽

Light And Electron Microscopy ◽

Scanning Laser ◽

Laser Exposure ◽

Confocal Scanning Laser Microscope ◽

Fluorescent Markers ◽

Before And After ◽

Staining Protocol ◽

Confocal Scanning ◽

Confocal Scanning Laser

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.

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Interface Science in Drinking Water Treatment - Theory and Application

10.1016/s1573-4285(06)x8069-9 ◽

2006 ◽

Keyword(s):

Drinking Water ◽

Water Treatment ◽

Drinking Water Treatment ◽

Interface Science ◽

Treatment Theory

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Modeling and Control of a Chemical Waste Water Treatment Plant

Computers & Chemical Engineering ◽

10.1016/s0098-1354(97)00171-3 ◽

1997 ◽

Vol 21 (1-2) ◽

pp. S947-S952 ◽

Cited By ~ 2

Author(s):

R Miller

Keyword(s):

Waste Water ◽

Water Treatment ◽

Waste Water Treatment ◽

Treatment Plant ◽

Water Treatment Plant ◽

Waste Water Treatment Plant ◽

Chemical Waste ◽

Modeling And Control ◽

And Control

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Optimization of Residence Time Distribution in Small Water Treatment Systems

Computational Fluid Dynamics ◽

10.1061/9780784415313.ch15 ◽

2019 ◽

pp. 123-130

Keyword(s):

Water Treatment ◽

Residence Time ◽

Time Distribution ◽

Residence Time Distribution ◽

Small Water

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To the issue of water treatment by hydrocyclone on the systems of the combined irrigation

Melioration and Water Management ◽

10.32962/0235-2524-2019-2-21-25 ◽

2020 ◽

Vol 0 (2) ◽

pp. 21-25

Author(s):

Nikolay Dubenok ◽

Andrey Novikov ◽

Sergei Borodychev ◽

Maria Lamskova

Keyword(s):

Water Treatment ◽

Organic Compounds ◽

Irrigation Water ◽

Irrigation System ◽

Petroleum Products ◽

Cylindrical Part ◽

Agricultural Landscapes ◽

Treatment Unit ◽

Drain Pipe ◽

Irrigation Network

At the stage of water treatment for irrigation systems, the efficiency capture coarse and fine mechanical impurities, as well as oil products and organic compounds affects the reliability of the equipment of the irrigation network and the safety of energy exchange processes in irrigated agricultural landscapes. The violation of work irrigation system can cause disruptions in irrigation schedules of agricultural crops, crop shortages, degradation phenomena on the soil and ecological tension. For the combined irrigation system, a water treatment unit has been developed, representing a hydrocyclone apparatus with a pipe filter in the case. For the capacity of 250 m3/h the main geometrical dimensions of hydrocyclone have been calculated. To organize the capture petroleum products and organic compounds, it has been proposed a modernization of a hydrocyclone unit, consisting in dividing the cylindrical part of the apparatus into two section. The first is section is for input irrigation water, the second one is for additional drainage of clarified irrigation water after sorption purification by the filter, placed on the disk and installed coaxially with the drain pipe and the pipe filter.

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