scholarly journals Emerging Techniques for Thalassemia Gene Detection

2018 ◽  
Author(s):  
Lingwen Zeng ◽  
Luxin Yu ◽  
Yinghui Zhang
Keyword(s):  
Gene Detection

scholarly journals Association of Ct Values from Real-Time PCR with Culture in Microbiological Clearance Samples for Shiga Toxin-Producing Escherichia coli (STEC)

2020 ◽  
Vol 8 (11) ◽  
pp. 1801
Author(s):  
Michael Bording-Jorgensen ◽  
Brendon D. Parsons ◽  
Gillian A.M. Tarr ◽  
Binal Shah-Gandhi ◽  
Colin Lloyd ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Shiga Toxin ◽  
Real Time Pcr ◽  
Gene Detection ◽  
Culture Positivity ◽  
Hands On ◽  
Culture Independent ◽  
Chromogenic Agar ◽  
Stool Samples

Shiga toxin-producing Escherichia coli (STEC) are associated with acute gastroenteritis worldwide, which induces a high economic burden on both healthcare and individuals. Culture-independent diagnostic tests (CIDT) in frontline microbiology laboratories have been implemented in Alberta since 2019. The objectives of this study were to determine the association between gene detection and culture positivity over time using STEC microbiological clearance samples and also to establish the frequency of specimen submission. Both stx genes’ amplification by real-time PCR was performed with DNA extracted from stool samples using the easyMAG system. Stools were inoculated onto chromogenic agar for culture. An association between gene detection and culture positivity was found to be independent of which stx gene was present. CIDT can provide rapid reporting with less hands-on time and technical expertise. However, culture is still important for surveillance and early cluster detection. In addition, stool submissions could be reduced from daily to every 3–5 days until a sample is negative by culture.

A Polymorphic Cluster in the 5′ Region of the Human Coagulation Factor VII Gene: Detection, Frequency, and Linkage Disequilibrium

1997 ◽  
Vol 88 (5) ◽  
pp. 445-448 ◽  
Author(s):  
Giorgio Dell'Acqua ◽  
Licia Iacoviello ◽  
Andria D'Orazio ◽  
Rosa Di Bitondo ◽  
Augusto Di Castelnuovo ◽  
...  
Keyword(s):  
Linkage Disequilibrium ◽  
Coagulation Factor ◽  
Factor Vii ◽  
Gene Detection ◽  
Coagulation Factor Vii ◽  
Detection Frequency ◽  
Human Coagulation Factor Vii

scholarly journals Culture-Independent Genotyping, Virulence and Antimicrobial Resistance Gene Identification of Staphylococcus aureus from Orthopaedic Implant-Associated Infections

2021 ◽  
Vol 9 (4) ◽  
pp. 707
Author(s):  
J. Christopher Noone ◽  
Fabienne Antunes Ferreira ◽  
Hege Vangstein Aamot
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Virulence Gene ◽  
Orthopaedic Implant ◽  
Metagenomic Sequencing ◽  
Gene Detection ◽  
Shotgun Metagenomic Sequencing ◽  
Antimicrobial Resistance Genes ◽  
Culture Independent

Our culture-independent nanopore shotgun metagenomic sequencing protocol on biopsies has the potential for same-day diagnostics of orthopaedic implant-associated infections (OIAI). As OIAI are frequently caused by Staphylococcus aureus, we included S. aureus genotyping and virulence gene detection to exploit the protocol to its fullest. The aim was to evaluate S. aureus genotyping, virulence and antimicrobial resistance genes detection using the shotgun metagenomic sequencing protocol. This proof of concept study included six patients with S. aureus-associated OIAI at Akershus University Hospital, Norway. Five tissue biopsies from each patient were divided in two: (1) conventional microbiological diagnostics and genotyping, and whole genome sequencing (WGS) of S. aureus isolates; (2) shotgun metagenomic sequencing of DNA from the biopsies. Consensus sequences were analysed using spaTyper, MLST, VirulenceFinder, and ResFinder from the Center for Genomic Epidemiology (CGE). MLST was also compared using krocus. All spa-types, one CGE and four krocus MLST results matched Sanger sequencing results. Virulence gene detection matched between WGS and shotgun metagenomic sequencing. ResFinder results corresponded to resistance phenotype. S. aureus spa-typing, and identification of virulence and antimicrobial resistance genes are possible using our shotgun metagenomics protocol. MLST requires further optimization. The protocol has potential application to other species and infection types.

DNA-Mediated Au–Au Dimer-Based Surface Plasmon Coupling Electrochemiluminescence Sensor for BRCA1 Gene Detection

2021 ◽  
Vol 93 (6) ◽  
pp. 3308-3314
Author(s):  
Qian Zhang ◽  
Yu Tian ◽  
Zihui Liang ◽  
Zizhun Wang ◽  
Shuping Xu ◽  
...  
Keyword(s):  
Surface Plasmon ◽  
Brca1 Gene ◽  
Plasmon Coupling ◽  
Gene Detection ◽  
Surface Plasmon Coupling

A pragmatic eLCR for an ultrasensitive detection of methicillin-resistant Staphylococcus aureus in joint synovial fluid: superior to qPCR

The Analyst ◽  
2021 ◽  
Author(s):  
Jinyuan Chen ◽  
Hongxiang Wei ◽  
Xinyu Fang ◽  
Yuanqing Cai ◽  
Zhenzhen Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Synovial Fluid ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Low Cost ◽  
Ultrasensitive Detection ◽  
Gene Detection ◽  
Methicillin Resistant ◽  
Cost Identification

A pragmatic electrochemical mecA gene detection method for a rapid, accurate and low-cost identification of MRSA in the joint synovial fluid of PJI patients.

scholarly journals Adenovirus 12 E1A gene detection by polymerase chain reaction in both the normal and coeliac duodenum.

Gut ◽  
1994 ◽  
Vol 35 (9) ◽  
pp. 1226-1232 ◽  
Author(s):  
M Lawler ◽  
P Humphries ◽  
C O'Farrelly ◽  
H Hoey ◽  
O Sheils ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gene Detection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
E1a Gene
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