A pragmatic eLCR for an ultrasensitive detection of methicillin-resistant Staphylococcus aureus in joint synovial fluid: superior to qPCR

The Analyst ◽  
2021 ◽  
Author(s):  
Jinyuan Chen ◽  
Hongxiang Wei ◽  
Xinyu Fang ◽  
Yuanqing Cai ◽  
Zhenzhen Zhang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Synovial Fluid ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Low Cost ◽  
Ultrasensitive Detection ◽  
Gene Detection ◽  
Methicillin Resistant ◽  
Cost Identification

A pragmatic electrochemical mecA gene detection method for a rapid, accurate and low-cost identification of MRSA in the joint synovial fluid of PJI patients.

scholarly journals Evaluation of a Rapid Culture-Based Screening Test for Detection of Methicillin Resistant Staphylococcus aureus

2011 ◽  
Vol 60 (3) ◽  
pp. 265-268
Author(s):  
BOUSHRA FWITY ◽  
RALF LOBMANN ◽  
ANDREAS AMBROSCH
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Detection Method ◽  
Latex Agglutination ◽  
Enrichment Broth ◽  
Methicillin Resistant ◽  
Selective Enrichment ◽  
Vitek 2 ◽  
Selective Enrichment Broth

The performance of a culture based assay, BacLite Rapid MRSA for the rapid detection (5 hours) of methicillin resistant Staphylococcus aureus (MRSA) from specimens (n = 377) obtained from nares, throat, wounds and perineum was investigated. Compared to culture based reference methods (chromogenic MRSA ID (bioMerieux)), selective enrichment broth, PBP2' latex agglutination (Oxoid) and VITEK 2 identification (bioMerieux), an overall sensitivity of 71% with a 82% specificity and a negative predictive value (NPV) of 95% was provided. The Baclite test is rapid and easy to use and has the advantage of a culture-based detection method for MRSA.

Low-cost colorimetric diagnostic screening assay for methicillin resistant Staphylococcus aureus

Talanta ◽  
2021 ◽  
Vol 225 ◽  
pp. 121946 ◽  
Author(s):  
Muhabat Adeola Raji ◽  
Raja Chinnappan ◽  
Atef Shibl ◽  
Ghadeer Suaifan ◽  
Karina Weber ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Low Cost ◽  
Screening Assay ◽  
Diagnostic Screening ◽  
Methicillin Resistant

scholarly journals Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

2016 ◽  
Vol 10 (08) ◽  
pp. 863-869 ◽  
Author(s):  
Bahija Serray ◽  
Salwa Oufrid ◽  
Imane Hannaoui ◽  
Fatna Bourjilate ◽  
Nabila Soraa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Gene Detection ◽  
Methicillin Resistant ◽  
Genes Encoding ◽  
Mother And Child ◽  
Icad Gene ◽  
Polymerase Chain ◽  
Child Hospital

Introduction: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilm-forming capacity in MRSA. Methodology: In this study, 53 isolates of MRSA, recovered from December 2010 to May 2014 in a mother and child hospital, CHU Mohamed VI in Marrakech, Morocco, were screened for the presence of bap and ica genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA, and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). The biofilm formation assay was performed in 96-well microtiter polystyrene plates. The presence of genes was determined by polymerase chain reaction (PCR). Results: An association was found between icaD gene detection and biofilm formation; 100% of the strains harbored icaD and produced biofilm. None of the isolates harbored bap or bbp. Furthermore, 96.23% isolates were positive for fnbA, 60.37% for eno, 43.39% for clfA and clfB, 11.32% for cna, 9.34% for ebpS, 5.66% for fib, and 1.89% for fnbA. Conclusions: Our findings showed that the MRSA carriage in Marrakech children was high. The genetic variations of adhesion genes require further investigation.

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