scholarly journals The Possibility of Recovering of Hydroxytyrosol from Olive Milling Wastewater by Enzymatic Bioconversion

10.5772/64774 ◽  
2016 ◽  
Author(s):  
Manel Hamza ◽  
Sami Sayadi
Keyword(s):  
Enzymatic Bioconversion

scholarly journals Enhancement of the apoptotic effects of Arctii Fructus extracts on cancer cells by the enzymatic bioconversion of lignans

2020 ◽  
Vol 8 (5) ◽  
pp. 2205-2213
Author(s):  
Jung‐Eun Kim ◽  
Nam Hyouck Lee ◽  
Young Ho Kim ◽  
Young Eon Kim ◽  
Tae‐Gyu Lim ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Enzymatic Bioconversion ◽  
Apoptotic Effects

High-yield enzymatic bioconversion of hydroquinone to α-arbutin, a powerful skin lightening agent, by amylosucrase

2012 ◽  
Vol 94 (5) ◽  
pp. 1189-1197 ◽  
Author(s):  
Dong-Ho Seo ◽  
Jong-Hyun Jung ◽  
Suk-Jin Ha ◽  
Hyun-Kug Cho ◽  
Dong-Hyun Jung ◽  
...  
Keyword(s):  
High Yield ◽  
Enzymatic Bioconversion ◽  
Skin Lightening

Interactions of nitroglycerin and sulfhydryl-donating compounds in coronary microvessels

1994 ◽  
Vol 266 (1) ◽  
pp. H291-H297 ◽  
Author(s):  
R. M. Wheatley ◽  
S. P. Dockery ◽  
M. A. Kurz ◽  
H. S. Sayegh ◽  
D. G. Harrison
Keyword(s):  
Redox Potential ◽  
Potent Inhibitor ◽  
Buthionine Sulfoximine ◽  
Glutathione Synthesis ◽  
Endothelin 1 ◽  
Intracellular Glutathione ◽  
Enzymatic Bioconversion

Previous studies have shown the effect of nitroglycerin on coronary microvessels < 100 microns in diameter is markedly enhanced by L-cysteine. These studies were performed to examine the mechanisms responsible for this effect. Under control conditions, nitroglycerin caused potent dilations of large (> 200 microns diam) coronary microvessels while having minimal effects on small (< 100 microns diam) coronary microvessels [peak relaxations 85 +/- 4 vs. 23 +/- 3% (mean +/- SE) of endothelin-1-constricted vessels, respectively]. L-Cysteine (100 microM) and N-acetylcysteine (100 microM) markedly enhanced nitroglycerin-induced relaxations of small coronary microvessels (peak relaxation 84 +/- 6 and 87 +/- 12%, respectively) while having no effect on relaxations of vessels > 100 microns. In contrast, neither L-methionine (100 microM) nor glutathione (100 microM) enhanced nitroglycerin's vasodilation of small coronary microvessels. The effects of L-cysteine and N-acetylcysteine on the augmentation of nitroglycerin vasodilatation in smaller coronary microvessels was abolished in the presence of buthionine sulfoximine (100 microM), a potent inhibitor of intracellular glutathione synthesis. Buthionine sulfoximine had no effect on the vasodilatation produced by nitroprusside. These data demonstrate that, in smaller coronary microvessels, L-cysteine and N-acetylcysteine enhance nitroglycerin-induced vasodilatation by increasing intracellular glutathione concentrations. Intracellular glutathione, formed from either L-cysteine or N-acetylcysteine, may participate in the formation of an intermediate of nitroglycerin biotransformation or may maintain a redox potential within coronary microvessels that favors enzymatic bioconversion of nitroglycerin.

Enzymatic Bioconversion of Cycloastragenol-6-O-β-D-glucoside into Cycloastragenol by a Novel Recombinant β-Glucosidase from Phycicoccus sp. Soil748

2020 ◽  
Vol 90 ◽  
pp. 81-88
Author(s):  
Leiyu Cheng ◽  
Han Zhang ◽  
Hao Liang ◽  
Xinxiao Sun ◽  
Xiaolin Shen ◽  
...  
Keyword(s):  
Enzymatic Bioconversion

Enzymatic Bioconversion of Cholesterol Using Glucosyltransferase and Methyltransferase Isolated from Streptomyces

2018 ◽  
Author(s):  
Niranjan Koirala
Keyword(s):  
Escherichia Coli ◽  
Amino Acids ◽  
Full Length ◽  
Streptomyces Avermitilis ◽  
Accession Number ◽  
Streptomyces Peucetius ◽  
Escherichia Coli Bl21 ◽  
Calculated Mass ◽  
Enzymatic Bioconversion ◽  
Full Length Gene

A sterol glycosyltransferases (SGT) gene (sav7185; accession number NP_828361) was isolated from Streptomyces avermitilis MA-4680. The full-length gene consists of 1284 nucleotides and encodes 427 amino acids with a calculated mass of 46.05 kDa. The gene was then cloned in pET28a vector and heterologously expressed in Escherichia coli BL21 (DE3) and was used for the biotransformation of cholesterol. This SGT protein showed favorable activity towards cholesterol tested in this study. Further, we tested the conversion of cholesterol to its methoxide using another Streptomyces O-methyltransferase (accession number KF420279). This O-methyltransferase (OMT) SpOMT2884, originating from Streptomyces peucetius ATCC 27952, was cloned, expressed, and applied for the production of methylated derivative. The GC-MS analysis confirmed the conversion of cholesterol into cholesterol-3-O-&beta;-D-glucoside and a novel cholesterol-3-O-methoxide. Hence, these Streptomyces SGT and OMT could find applications for the derivatization of pharmaceutically significant sterols.

scholarly journals Enzymatic bioconversion of feather waste with keratinases of Bacillus cereus PCM 2849

2019 ◽  
Vol 21 (3) ◽  
pp. 53-59 ◽  
Author(s):  
Dominika P. Ciurko ◽  
Wojciech Łaba ◽  
Michał Piegza ◽  
Piotr Juszczyk ◽  
Anna Choińska-Pulit ◽  
...  
Keyword(s):  
Amino Acids ◽  
Bacillus Cereus ◽  
Reaction Temperature ◽  
Process Conditions ◽  
Box Behnken Design ◽  
Chicken Feathers ◽  
Disulphide Bonds ◽  
Branched Chain ◽  
Enzymatic Bioconversion ◽  
Hydrolysis Of

Abstract Enzymatic preparation from culture of keratinolytic Bacillus cereus PCM 2849 was applied for hydrolysis of whole chicken feathers, after sulphitolytic pretreatment. This process was optimized using a three-factor Box-Behnken design, where the effect of substrate concentration, sulphite concentration during pretreatment and reaction temperature was evaluated on the release of amino acids. Obtained results revealed the highest impact of reaction temperature, followed by substrate content and sulphite during pretreatment. Optimal process conditions were established, i.e. temperature 44.4°C, feathers 4.7% and treatment with 25.3 mM sulphite. Amino acid composition of the obtained hydrolysate was analyzed. Glutamic acid (9.21 g·kg−1) and proline were dominant, however significant amount of branched-chain amino acids was also observed. The FTIR analysis of residual substrate revealed the cleavage of disulphide bonds in keratin through the presence of thioester residues. The absence of reduced cysteine residues was confirmed, along with minor changes in proportions of keratin substructures.

scholarly journals Lipase spesifik 1,3-gliserida dari fungi lokal untuk biokonversi CPO menjadi diasilgliserol Specific lipase of 1,3-glyceride from indigenous fungi for bioconversion of CPO to produce diacylglycerol

2016 ◽  
Vol 76 (1) ◽  
Author(s):  
. TRI-PANJI ◽  
. SUHARYANTO ◽  
Nining ARINI
Keyword(s):  
Fatty Acids ◽  
Optimum Condition ◽  
Economic Value ◽  
Lipase Production ◽  
High Price ◽  
Local Source ◽  
Research Activities ◽  
Local Sources ◽  
Enzymatic Bioconversion ◽  
Supporting Materials

SummaryDownstream industry of palm oil producing specialty oil with higher economic value compared to that of CPO in Indonesia is less developed due to technical obstacle and the availability of supporting materials. Specific lipase 1,3-glyceride for example which is used for oleochemical processing of healthy oil production is still imported with relatively high price.  Healthy oil can be made from CPO bioconversion using the enzyme that produces oil rich in diacylglycerol (DAG). Although research on the production and the use of lipase has been well studied, production of specific lipase from microbes of local source is still very limited.  This article reports one part of the series of the research activities on bioprocess and genetic engineering approaches to produce specific lipase for bioconversion of CPO i.e optimization of 1,3-glyceride-spesific lipase production from fungi selected from local sources. Based on the fluorescence zone on the screening media, of the twenty isolates collection, it was found that P6 isolate, thereafter indentified as Neurospora sitophila, has the highest activity of 1,3-glyceride-specific lipase. The lipase of N.  sitophila was able to catalyze glycerolysis of triacylglycerol (TAG) in CPO to produce DAG. The bioconversion products of lipase yielding ratio of DAG/TAG was higher than ratio of free fatty acids (FFA)/TAG (0.12 > 0.08). The optimum condition of the enzymatic bioconversion was at 40 oC, pH 6, and 10-day incubation. The primary fatty acids on the DAG were oleic (56.2%), palmitic (40.0%), and myristic (2.7%) acids. The decrease of palmitic acid on DAG compared to on TAG, indicated that the lipase of N. sitophila worked relatively specific at C1 or C3 of the TAG.Kurang berkembangnya industri hilir yang menghasilkan minyak khusus yang nilainya berlipat dibandingkan CPO antara lain karena hambatan teknis dan ketersediaan bahan pendukungnya. Lipase spesifik 1,3-gliserida misalnya, yang digunakan untuk produksi minyak kesehatan, masih diimpor dengan harga relatif tinggi. Minyak kesehatan dapat diproduksi dari biokonversi CPO dengan lipase spesifik 1,3-gliserida hingga diperoleh minyak yang kaya kandungan diasilgliserol (DAG). Tulisan ini melaporkan optimasi aktivitas lipase spesifik 1,3-gliserida dari fungi isolat lokal terpilih. Berdasarkan zona fluoresens pada medium penapis lipase, dari 20 isolat fungi yang diuji isolat P6 yang kemudian diidentifikasi sebagai Neurospora sitophila memiliki aktivitas tertinggi dan bersifat spesifik 1,3-gliserida. Lipase N. sitophilamampu mengkatalisis gliserolisis triasilgliserol (TAG) dalam CPO untuk menghasilkan DAG. Lipase tersebut menghasilkan nilai perban-dingan DAG/TAG  lebih  besar  dari nilai perbandingan asam lemak bebas (ALB)/TAG (0,12 > 0,08). Kondisi optimum biokonversi enzimatis ini terjadi pada suhu 40 oC, pH 6, dan waktu inkubasi selama 10 hari. Asam lemak utama penyusun DAG adalah asam oleat (56,2%), palmitat (40,0%), dan miristat (2,7%). Berkurangnya asam palmitat pada DAG dibanding pada TAG menunjukkan bahwa lipase N. sitophila bekerja secara relatif spesifik pada C1 atau C3 dari gliserida.

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