scholarly journals Microsatellite Markers Confirm Self‐Pollination and Autogamy in Wild Populations of Vanilla mexicana Mill. (syn. V. inodora) (Orchidaceae) in the Island of Guadeloupe

10.5772/64674 ◽  
2016 ◽  
Author(s):  
Rodolphe Laurent Gigant ◽  
Narindra Rakotomanga ◽  
Chloe Goulié ◽  
Denis Da Silva ◽  
Nicolas Barre ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Wild Populations ◽  
Self Pollination

scholarly journals Identifying Pollen Donors and Success Rate of Individual Pollinizers in European Plum (Prunus domestica L.) Using Microsatellite Markers

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 264
Author(s):  
Meland ◽  
Frøynes ◽  
Fotiric Akšić ◽  
Pojskić ◽  
Kalamujić Stroil ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Success Rate ◽  
Genetic Characterization ◽  
Prunus Domestica ◽  
Tissue Samples ◽  
Self Pollination ◽  
Self Fertilization ◽  
High Level ◽  
Very High ◽  
Simple Sequence

European plum cultivars (Prunus domestica L.) are hexaploid and partially self-fertile or self-sterile requiring compatible pollinizers with overlapping bloom times. Therefore, inter-planting of different pollinizer cultivars is recommended. In order to identify successful pollinizers of the plum cultivars ‘Edda’, ‘Opal’ (self-fertile), ‘Jubileum’, ‘Reeves’, ‘Mallard’, ‘Avalon’, ‘Cacanska Lepotica’ (self-fertile), and ‘Valor’, 60 fruits per cultivar were collected from nine orchards in 2017 and 2018, all of which were located in Ullensvang, western Norway. DNA extraction was subsequently conducted from the obtained embryos, followed by genetic characterization using seven microsatellite markers. Tissue samples from all possible pollinizers were collected during the summer of 2017 and the same DNA approach was conducted. Results showed that ‘Opal’ was the most successful pollinizer among the investigated plum cultivars. The main exception was ‘Cacanska Lepotica’, which consistently displayed very high level of self-pollination. The most successful foreign pollinizer of ‘Opal’ was ‘Mallard’. However, in more than two thirds of embryos extracted from ‘Opal’ fruits self-fertilization was determined. ‘Reeves’ was identified as the most successful pollinizer among embryos collected from ‘Valor’. Among the five cultivars (‘Edda’, ‘Jubileum’, ‘Reeves’, ‘Mallard’, and ‘Avalon’) that did not display self-pollination, the pollinizer success rate of ‘Opal’, ranged from 36.5% (‘Mallard’) to 93.5% (‘Edda’) in 2017, while in 2018 this rate ranged from 43.5% (‘Jubileum’ and ‘Reeves’) up to 96.5% (‘Edda’). Overall, genotyping embryos using SSRs (simple sequence repeats) proved an effective method in determining the success rate of individual pollinizers among European plum cultivars.

Genetic relatedness and cultivar identification in a valuable garden species, Hesperantha coccinea ( Schizostylis coccinea)

2009 ◽  
Vol 7 (03) ◽  
pp. 281-290
Author(s):  
Kirsten Wolff ◽  
Sabina Knees ◽  
Suzanne Cubey
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Dna Fingerprinting ◽  
Genetic Relatedness ◽  
Cultivar Identification ◽  
Wild Populations ◽  
Multilocus Genotypes ◽  
Expected Heterozygosity ◽  
Garden Plants ◽  
Garden Plant

DNA fingerprinting using microsatellites is a useful aid in cultivar identification, but has rarely been applied to garden plants. Eleven microsatellite markers were developed for the valuable garden plantHesperanthacoccinea(Schizostylis coccinea), and used to determine relatedness of accessions. Several accessions, described as separate cultivars, appeared to have identical genotypes. Among the 53 accessions tested, there were 34 unique multilocus genotypes. The level of polymorphism detected in the cultivars was high, with on average seven alleles per locus and an average expected heterozygosity of 0.72 across loci. It is clear from the genotypes that a large proportion of the cultivars are closely related to each other. The resulting markers can now be used to generate a complete database of all known cultivars of the species and to detect essentially derived cultivars. As an extension of this study, the markers identified here could also inform us about the genetic diversity in wild populations.

Development and characterization of polymorphic microsatellite markers for Spathoglottis plicata (Orchidaceae)

2018 ◽  
Vol 16 (6) ◽  
pp. 572-575
Author(s):  
Chiuan-Yu Li ◽  
Chi-Chun Huang ◽  
Chaur-Tzuhn Chen ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Primers ◽  
Wild Populations ◽  
Terrestrial Orchid ◽  
Chain Reaction ◽  
Expected Heterozygosity ◽  
Genetic Patterns ◽  
Polymerase Chain ◽  
Polymorphic Microsatellite

AbstractWe developed novel and polymorphic microsatellite primers for Spathoglottis plicata, a tropical and subtropical terrestrial orchid, to investigate the genetic patterns and population structure among wild populations, and also to identify the varieties and hybrids of S. plicata in horticultural industry. The 12 novel microsatellites from S. plicata were developed by using polymerase chain reaction (PCR)-based isolation of microsatellite arrays. These markers that were successfully PCR amplified exhibited polymorphisms in S. plicata. The number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content values across loci ranged from 2.000 to 8.000, 0.000 to 0.756, 0.208 to 0.813 and 0.405 to 0.805 in total populations, respectively. The newly developed microsatellite markers exhibited variation in S. plicata. These markers can be used as a tool to further investigate the genetic diversity, conservation genetics and variety/hybrid identification of S. plicata.

scholarly journals Development of Microsatellite Markers forViscum coloratum(Santalaceae) and Their Application to Wild Populations

2017 ◽  
Vol 5 (1) ◽  
pp. 1600102 ◽  
Author(s):  
Bo-Yun Kim ◽  
Han-Sol Park ◽  
Soonok Kim ◽  
Young-Dong Kim
Keyword(s):  
Microsatellite Markers ◽  
Wild Populations

scholarly journals Obtaining interspecific hybrids, and molecular analysis by microsatellite markers in grapevine

2011 ◽  
Vol 46 (11) ◽  
pp. 1480-1488 ◽  
Author(s):  
Mariane Ruzza Schuck ◽  
Luiz Antonio Biasi ◽  
Ada Michele Mariano ◽  
Bernardo Lipski ◽  
Summaira Riaz ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Interspecific Hybridization ◽  
Molecular Analysis ◽  
Interspecific Hybrids ◽  
Female Parent ◽  
F1 Hybrids ◽  
Reciprocal Crosses ◽  
Self Pollination ◽  
Vitis Labruscana ◽  
Muscadinia Rotundifolia

The objective of this work was to assess the potential of interspecific hybridization of Vitis labruscana and Muscadinia rotundifolia by using artificial cross-pollinations. Microsatellite markers were used to confirm interspecific hybridizations and the identity of the parental genotypes. In crosses in which M. rotundifolia was used as the female parent, no true hybrids were obtained. In the reciprocal crosses, 114 seedlings were identified as true V. labruscana x M. rotundifolia hybrids. Self pollination occurred in direct and in reciprocal crosses. The crossings between 'Bordo' x 'Carlos', 'Magnolia', 'Regale' and' Roanoke', and between' Isabel' x 'Bountiful', 'Carlos', 'Magnolia', 'Regale' and 'Roanoke' were confirmed. The 15 markers evaluated showed that two M. rotundifolia parental genotypes had the same fingerprint profile, indicating a like lyplanting error. The success of hybridization depends mainly on the species and on the cultivar used as the female parent. Microsatellite markers are efficient to confirm the paternity of interspecific F1 hybrids and to determine the correct identity of M. rotundifolia cultivars.

scholarly journals Microsatellite markers show distinctiveness of released and wild grey partridges in Finland

2012 ◽  
Vol 35 (2) ◽  
pp. 419-428
Author(s):  
T. Liukkonen ◽  
◽  
L. Kvist ◽  
S. Mykrä ◽  
◽  
...  
Keyword(s):  
Population Structure ◽  
Great Britain ◽  
Genetic Structure ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
First Generation ◽  
Wild Populations ◽  
First Generation Migrants ◽  
Native Birds

The main aim of this study was to study whether the present game farm stocks used for releases to the wild in Finland are similar to wild populations in their genetic structure, and if not, whether the wild populations show any signs of hybridisation. A total of 301 feather samples and ten microsatellite loci were used. Samples were collected from France, Great Britain, Finland (wild and captive) and Greece. We estimated pairwise FST–values between study populations, examined population structure and identified possible first generation migrants. Pairwise FST–values indicated structuring among studied populations. Results indicate that the farm stock used for releases deviates from the wild populations. No signs of hybridisation between the released and native birds were detected.

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