scholarly journals Development of Microsatellite Markers forViscum coloratum(Santalaceae) and Their Application to Wild Populations

2017 ◽  
Vol 5 (1) ◽  
pp. 1600102 ◽  
Author(s):  
Bo-Yun Kim ◽  
Han-Sol Park ◽  
Soonok Kim ◽  
Young-Dong Kim
Keyword(s):  
Microsatellite Markers ◽  
Wild Populations

Genetic relatedness and cultivar identification in a valuable garden species, Hesperantha coccinea ( Schizostylis coccinea)

2009 ◽  
Vol 7 (03) ◽  
pp. 281-290
Author(s):  
Kirsten Wolff ◽  
Sabina Knees ◽  
Suzanne Cubey
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Dna Fingerprinting ◽  
Genetic Relatedness ◽  
Cultivar Identification ◽  
Wild Populations ◽  
Multilocus Genotypes ◽  
Expected Heterozygosity ◽  
Garden Plants ◽  
Garden Plant

DNA fingerprinting using microsatellites is a useful aid in cultivar identification, but has rarely been applied to garden plants. Eleven microsatellite markers were developed for the valuable garden plantHesperanthacoccinea(Schizostylis coccinea), and used to determine relatedness of accessions. Several accessions, described as separate cultivars, appeared to have identical genotypes. Among the 53 accessions tested, there were 34 unique multilocus genotypes. The level of polymorphism detected in the cultivars was high, with on average seven alleles per locus and an average expected heterozygosity of 0.72 across loci. It is clear from the genotypes that a large proportion of the cultivars are closely related to each other. The resulting markers can now be used to generate a complete database of all known cultivars of the species and to detect essentially derived cultivars. As an extension of this study, the markers identified here could also inform us about the genetic diversity in wild populations.

Development and characterization of polymorphic microsatellite markers for Spathoglottis plicata (Orchidaceae)

2018 ◽  
Vol 16 (6) ◽  
pp. 572-575
Author(s):  
Chiuan-Yu Li ◽  
Chi-Chun Huang ◽  
Chaur-Tzuhn Chen ◽  
Kuo-Hsiang Hung
Keyword(s):  
Microsatellite Markers ◽  
Microsatellite Primers ◽  
Wild Populations ◽  
Terrestrial Orchid ◽  
Chain Reaction ◽  
Expected Heterozygosity ◽  
Genetic Patterns ◽  
Polymerase Chain ◽  
Polymorphic Microsatellite

AbstractWe developed novel and polymorphic microsatellite primers for Spathoglottis plicata, a tropical and subtropical terrestrial orchid, to investigate the genetic patterns and population structure among wild populations, and also to identify the varieties and hybrids of S. plicata in horticultural industry. The 12 novel microsatellites from S. plicata were developed by using polymerase chain reaction (PCR)-based isolation of microsatellite arrays. These markers that were successfully PCR amplified exhibited polymorphisms in S. plicata. The number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content values across loci ranged from 2.000 to 8.000, 0.000 to 0.756, 0.208 to 0.813 and 0.405 to 0.805 in total populations, respectively. The newly developed microsatellite markers exhibited variation in S. plicata. These markers can be used as a tool to further investigate the genetic diversity, conservation genetics and variety/hybrid identification of S. plicata.

scholarly journals Microsatellite markers show distinctiveness of released and wild grey partridges in Finland

2012 ◽  
Vol 35 (2) ◽  
pp. 419-428
Author(s):  
T. Liukkonen ◽  
◽  
L. Kvist ◽  
S. Mykrä ◽  
◽  
...  
Keyword(s):  
Population Structure ◽  
Great Britain ◽  
Genetic Structure ◽  
Microsatellite Markers ◽  
Microsatellite Loci ◽  
First Generation ◽  
Wild Populations ◽  
First Generation Migrants ◽  
Native Birds

The main aim of this study was to study whether the present game farm stocks used for releases to the wild in Finland are similar to wild populations in their genetic structure, and if not, whether the wild populations show any signs of hybridisation. A total of 301 feather samples and ten microsatellite loci were used. Samples were collected from France, Great Britain, Finland (wild and captive) and Greece. We estimated pairwise FST–values between study populations, examined population structure and identified possible first generation migrants. Pairwise FST–values indicated structuring among studied populations. Results indicate that the farm stock used for releases deviates from the wild populations. No signs of hybridisation between the released and native birds were detected.

scholarly journals Population Genetic Analysis for Stock Enhancement of Silver Sea Bream (Rhabdosargus sarba) in Taiwan

Fishes ◽  
2020 ◽  
Vol 5 (2) ◽  
pp. 19 ◽  
Author(s):  
Te-Hua Hsu ◽  
Chang-Wen Huang ◽  
Hung-Tai Lee ◽  
Yi-Hsuan Kuo ◽  
Kwang-Ming Liu ◽  
...  
Keyword(s):  
Microsatellite Markers ◽  
Genetic Effect ◽  
Stock Enhancement ◽  
Unintended Consequences ◽  
Sea Bream ◽  
Type I ◽  
Type Ii ◽  
Wild Populations ◽  
Population Genetic Analysis ◽  
Silver Sea Bream

Stock enhancement is a method for replenishing depleted wild finfish populations by supplementing them with hatchery-raised fish. In Taiwan, silver sea bream (Rhabdosargus sarba) is a predominant commercial species involved in stock enhancement projects. Although management agencies conduct stock enhancement projects, there are a lot of private releases without records. Stock enhancement is performed by the private aquaculture sector without accurate genetic records, potentially leading to unintended consequences for wild populations. We analyzed the genetics of 459 wild and 701 hatchery-reared specimens from nine batches produced by various hatcheries. Wild and hatchery-reared samples could be considered two separate clades by using a set of stable and informative microsatellite markers including type I (from gene introns and 3′UTR) and type II markers (randomly picked up from genome). Type I microsatellite markers could more sensitively reflect the loss of genetic diversity more than type II markers in the domestication process. All specimens were considered native by using mtDNA COI and microsatellites. The genetic composition of the wild population is relatively simple, and the estimated low contribution rate of the hatchery stock (1.3–10.9%; 6–50/459) indicated a weak but significant genetic effect of stock enhancement. Therefore, establishing standards for the stock enhancement of silver sea bream for more effective supplementation of wild populations is imperative.

Genetic diversity comparisons of wild populations of Nelumbo nucifera (Nelumbonaceae) in Russia and China using microsatellite markers

2020 ◽  
Vol 306 (5) ◽  
Author(s):  
Zhi-Zhong Li ◽  
Md. Rabiul Islam ◽  
Shi-Xu Huang ◽  
Dan Yang ◽  
Yeshitila Mekbib ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Microsatellite Markers ◽  
Nelumbo Nucifera ◽  
Wild Populations
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