scholarly journals Nontargeted Metabolite Profiling of Induced Pluripotent Stem Cells (iPSCs) Derived Neural Cells: Insights Into Mechanisms of Brain Diseases

10.5772/62851 ◽  
2016 ◽  
Author(s):  
Šárka Lehtonen ◽  
Marko Lehtonen ◽  
Jari Koistinaho
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Metabolite Profiling ◽  
Brain Diseases ◽  
Neural Cells ◽  
Induced Pluripotent

scholarly journals Induced pluripotent stem cells in psychiatry: a critical review

2021 ◽  
Author(s):  
Alejandro De Los Angeles ◽  
Kristen Brennand ◽  
Nicola Hall ◽  
Michael Fernando ◽  
Paul Harrison ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Statistical Power ◽  
Model Systems ◽  
Neural Cells ◽  
Case Control Studies ◽  
Pathophysiological Mechanisms ◽  
Induced Pluripotent ◽  
Cellular Phenotypes

A key challenge in psychiatry research is the development of high-fidelity model systems that can be experimentally manipulated to explore and test pathophysiological mechanisms of illness. In this respect, the emerging capacity to derive neural cells and circuits from human induced pluripotent stem cells (iPSCs) has generated significant excitement. This review aims to provide a critical appraisal of the potential for iPSCs in illuminating pathophysiological mechanisms and to situate them in the wider technical landscape. We discuss the selection of iPSC phenotypes relevant to psychiatry, the information that researchers can draw on to help guide these decisions, and how researchers choose between the use of 2D cultures, vs. more complex 3D model systems. We discuss the strengths and limitations of current models, and the challenges and opportunities that they present. Finally, we discuss the potential of iPSC-based model systems for clarifying the mechanisms underlying genetic risk for psychiatry, and the steps that will be needed to ensure that robust and reliable conclusions can be drawn. We argue that, whilst iPSC-based models are ideally placed to study fundamental processes occurring within and between neural cells, they are often less well-suited for case-control studies, given issues relating to statistical power and the challenges in identifying which cellular phenotypes are meaningful at the level of the whole individual. Our aim is to highlight the importance of considering the hypotheses of a given study to guide decisions about which, if any, iPSC-based system is most appropriate to address it.

Induced Pluripotent Stem Cells in Brain Diseases

2012 ◽  
Author(s):  
Vivi M. Heine ◽  
Stephanie Dooves ◽  
Dwayne Holmes ◽  
Judith Wagner
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Brain Diseases ◽  
Induced Pluripotent

scholarly journals FOXOs modulate proteasome activity in human-induced pluripotent stem cells of Huntington’s disease and their derived neural cells

2017 ◽  
Vol 26 (22) ◽  
pp. 4416-4428 ◽  
Author(s):  
Yanying Liu ◽  
Fangfang Qiao ◽  
Patricia C Leiferman ◽  
Alan Ross ◽  
Evelyn H Schlenker ◽  
...  
Keyword(s):  
Stem Cells ◽  
Huntington's Disease ◽  
Induced Pluripotent Stem Cells ◽  
Huntington’S Disease ◽  
Pluripotent Stem Cells ◽  
Proteasome Activity ◽  
Neural Cells ◽  
Induced Pluripotent

scholarly journals 3D Bioprinted Human Cortical Neural Constructs Derived from Induced Pluripotent Stem Cells

2019 ◽  
Vol 8 (10) ◽  
pp. 1595 ◽  
Author(s):  
Salaris ◽  
Colosi ◽  
Brighi ◽  
Soloperto ◽  
Turris ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cortical Neurons ◽  
Early Stage ◽  
Network Activity ◽  
Neural Cells ◽  
Human Ipscs ◽  
Wide Range ◽  
Induced Pluripotent

Bioprinting techniques use bioinks made of biocompatible non-living materials and cells to build 3D constructs in a controlled manner and with micrometric resolution. 3D bioprinted structures representative of several human tissues have been recently produced using cells derived by differentiation of induced pluripotent stem cells (iPSCs). Human iPSCs can be differentiated in a wide range of neurons and glia, providing an ideal tool for modeling the human nervous system. Here we report a neural construct generated by 3D bioprinting of cortical neurons and glial precursors derived from human iPSCs. We show that the extrusion-based printing process does not impair cell viability in the short and long term. Bioprinted cells can be further differentiated within the construct and properly express neuronal and astrocytic markers. Functional analysis of 3D bioprinted cells highlights an early stage of maturation and the establishment of early network activity behaviors. This work lays the basis for generating more complex and faithful 3D models of the human nervous systems by bioprinting neural cells derived from iPSCs.

scholarly journals Generation of Neural Cells from DM1 Induced Pluripotent Stem Cells As Cellular Model for the Study of Central Nervous System Neuropathogenesis

2013 ◽  
Vol 15 (2) ◽  
pp. 166-177 ◽  
Author(s):  
Guangbin Xia ◽  
Katherine E. Santostefano ◽  
Marianne Goodwin ◽  
Jilin Liu ◽  
S.H. Subramony ◽  
...  
Keyword(s):  
Stem Cells ◽  
Central Nervous System ◽  
Nervous System ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Cells ◽  
Cellular Model ◽  
Induced Pluripotent
Sign in / Sign up

Export Citation Format

Share Document