scholarly journals Monitoring Photosynthesis by In Vivo Chlorophyll Fluorescence: Application to High-Throughput Plant Phenotyping

10.5772/62391 ◽  
2016 ◽  
Author(s):  
Jorge Marques da Silva
Keyword(s):  
Chlorophyll Fluorescence ◽  
High Throughput ◽  
Plant Phenotyping ◽  
In Vivo Chlorophyll Fluorescence

scholarly journals In vivo diagnostics of early abiotic plant stress response via Raman spectroscopy

2017 ◽  
Vol 114 (13) ◽  
pp. 3393-3396 ◽  
Author(s):  
Narangerel Altangerel ◽  
Gombojav O. Ariunbold ◽  
Connor Gorman ◽  
Masfer H. Alkahtani ◽  
Eli J. Borrego ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Stress Response ◽  
High Throughput ◽  
Plant Stress ◽  
Spectroscopic Technique ◽  
Plant Phenotyping ◽  
Plant Stress Response ◽  
Concentration Changes ◽  
Concentration Levels

Development of a phenotyping platform capable of noninvasive biochemical sensing could offer researchers, breeders, and producers a tool for precise response detection. In particular, the ability to measure plant stress in vivo responses is becoming increasingly important. In this work, a Raman spectroscopic technique is developed for high-throughput stress phenotyping of plants. We show the early (within 48 h) in vivo detection of plant stress responses. Coleus (Plectranthus scutellarioides) plants were subjected to four common abiotic stress conditions individually: high soil salinity, drought, chilling exposure, and light saturation. Plants were examined poststress induction in vivo, and changes in the concentration levels of the reactive oxygen-scavenging pigments were observed by Raman microscopic and remote spectroscopic systems. The molecular concentration changes were further validated by commonly accepted chemical extraction (destructive) methods. Raman spectroscopy also allows simultaneous interrogation of various pigments in plants. For example, we found a unique negative correlation in concentration levels of anthocyanins and carotenoids, which clearly indicates that plant stress response is fine-tuned to protect against stress-induced damages. This precision spectroscopic technique holds promise for the future development of high-throughput screening for plant phenotyping and the quantification of biologically or commercially relevant molecules, such as antioxidants and pigments.

scholarly journals An LED-based multi-actinic illumination system for the high throughput study of photosynthetic light responses

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5589
Author(s):  
João Serôdio ◽  
William Schmidt ◽  
Jörg C. Frommlet ◽  
Gregor Christa ◽  
Matthew R. Nitschke
Keyword(s):  
Chlorophyll Fluorescence ◽  
High Throughput ◽  
Light Stress ◽  
Limiting Factor ◽  
Phenotypic Traits ◽  
Light Responses ◽  
Illumination System ◽  
Digital Manipulation ◽  
Multiple Samples

The responses of photosynthetic organisms to light stress are of interest for both fundamental and applied research. Functional traits related to the photoinhibition, the light-induced loss of photosynthetic efficiency, are particularly interesting as this process is a key limiting factor of photosynthetic productivity in algae and plants. The quantitative characterization of light responses is often time-consuming and calls for cost-effective high throughput approaches that enable the fast screening of multiple samples. Here we present a novel illumination system based on the concept of ‘multi-actinic imaging’ of in vivo chlorophyll fluorescence. The system is based on the combination of an array of individually addressable low power RGBW LEDs and custom-designed well plates, allowing for the independent illumination of 64 samples through the digital manipulation of both exposure duration and light intensity. The illumination system is inexpensive and easily fabricated, based on open source electronics, off-the-shelf components, and 3D-printed parts, and is optimized for imaging of chlorophyll fluorescence. The high-throughput potential of the system is illustrated by assessing the functional diversity in light responses of marine macroalgal species, through the fast and simultaneous determination of kinetic parameters characterizing the response to light stress of multiple samples. Although the presented illumination system was primarily designed for the measurement of phenotypic traits related to photosynthetic activity and photoinhibition, it can be potentially used for a number of alternative applications, including the measurement of chloroplast phototaxis and action spectra, or as the basis for microphotobioreactors.

Changes of Excitation Spectra of in vivo Chlorophyll Fluorescence during Induction of Photosynthesis

1993 ◽  
Vol 48 (1-2) ◽  
pp. 46-51 ◽  
Author(s):  
Wieslaw I. Gruszecki ◽  
Zbigniew Krupa
Keyword(s):  
Energy Transfer ◽  
Chlorophyll Fluorescence ◽  
Quantum Yield ◽  
Dark Adaptation ◽  
Fluorescence Quantum Yield ◽  
Excitation Energy Transfer ◽  
Reaction Centers ◽  
Excitation Spectra ◽  
In Vivo Chlorophyll Fluorescence

Excitation spectra of chlorophyll fluorescence from intact rye leaves were registered at different steps of the induction of photosynthesis after dark adaptation. Analysis of these spectra indicates that at least two processes related to spectroscopic features are responsible for a fluorescence quenching. The first one, active during the first 100 s of illumination, was interpreted to consists in an overall decrease of the fluorescence quantum yield of antenna pigments and chlorophylls, in particular close to the reaction centers. The second type of a fluorescence decrease (between 100 s and 300 s of illumination) was found to be in large extent related to decrease of the rate of an excitation energy transfer between accessory xanthophyll pigments and chlorophylls emitting fluorescence. This latter molecular mechanism is discussed as being related to violaxanthin availability to de-epoxidation in the xanthophyll cycle.

Photoprotective function of chloroplast avoidance movement: In vivo chlorophyll fluorescence study

2010 ◽  
Vol 167 (9) ◽  
pp. 709-716 ◽  
Author(s):  
Olga Sztatelman ◽  
Andrzej Waloszek ◽  
Agnieszka Katarzyna Banaś ◽  
Halina Gabryś
Keyword(s):  
Chlorophyll Fluorescence ◽  
Fluorescence Study ◽  
In Vivo Chlorophyll Fluorescence
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