scholarly journals Aminosilane Functionalization and Cytotoxicity Effects of Upconversion Nanoparticles Y2O3 and Gd2O3 Co-Doped with Yb3+and Er3+

10.5772/62252 ◽  
2016 ◽  
Vol 3 ◽  
pp. 1 ◽  
Author(s):  
Dalia Holanda Chavez ◽  
Karla Juarez-Moreno ◽  
Gustavo Alonso Hirata
Keyword(s):  
Cancer Cells ◽  
Cell Lines ◽  
Near Infrared ◽  
Colorectal Adenocarcinoma ◽  
Colorimetric Assay ◽  
Sol Gel ◽  
Upconversion Nanoparticles ◽  
Cervix Carcinoma ◽  
Visible Radiation ◽  
Co Doped

In this study, luminescent upconversion nanoparticles (UCNPs) Y2O3 and Gd2O3 co-doped with Yb3+ and Er3+ were prepared by the sol-gel method (SG). These NPs are able to absorb near infrared photons and upconvert them into visible radiation with a direct application in bioimaging, as an important tool to diagnose and visualize cancer cells. The UCNPs were coated with a thin silica shell and functionalized with amino groups for further folic acid conjugation to allow their interaction with folate ligands on the cell surface. Their physical properties were analysed by Transmission Electron Microscopy (TEM), Fourier transform infrared spectroscopy (FTIR) and photoluminescence (PL) measurements. The PL results revealed excellent luminescence properties on all core-shell UCNPs. Cytotoxicity experiments with concentrations of bare and aminosilane coated/functionalized UCNPs between 0.001 μg/mL to 1 μg/mL were tested on two different cell lines from human cervix carcinoma (HeLa) and human colorectal adenocarcinoma (DLD-1) with a colorimetric assay based on the reduction of MTT reagent (methy-134-thiazolyltetrazolium). The assays show that some concentrations of bare UCNPs were cytotoxic for cervical adenocarcinoma cells (HeLa); however, for human colorectal adenocarcinoma all UCNPs are non-cytotoxic. After UCNPs functionalization with silica-aminosilane (APTES/TEOS), all of the nanoparticles tested were found to be non-cytotoxic for both cell lines. The UCNPs functionalized in this work can be further conjugated with specific ligands and used as biolabels for detection of cancer cells.

Silica coated, aminosilane functionalization, upconversion emission and cytotoxicity in cancer cell lines of the nanoparticles Y2O3 and Gd2O3 co-doped with Yb3+ and Er3+

2016 ◽  
Vol 1817 ◽  
Author(s):  
D. Chávez ◽  
K. Juárez-Moreno ◽  
G.A. Hirata
Keyword(s):  
Cell Lines ◽  
Near Infrared ◽  
Colorectal Adenocarcinoma ◽  
Colorimetric Assay ◽  
Sol Gel ◽  
Luminescence Spectra ◽  
Core Shell ◽  
Cervix Carcinoma ◽  
Shell Nanoparticles ◽  
Co Doped

ABSTRACTY2O3 and Gd2O3 upconversion nanoparticles (UCN) co-doped with Yb3+ and Er3+ can absorb and upconvert near infrared (NIR) radiation into visible light. These UCN find application in bioimaging, as an important tool to diagnose and visualize cancer cells. The UCN can be used as biolabels to identify the cells; the nanoparticles can be coated and functionalized with ligands that bind to receptors on the surface of the cell. In this project, the UCN were synthesized by sol-gel method and subsequently coated with a thin silica shell by using the Stöber method. The core-shell UCN were functionalized with amine group to enable folic acid conjugation. The functionalized core-shell nanoparticles were analyzed by transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and luminescence measurements. Concentrations of bare and coated/functionalized UCN between 0.001 µg/mL and 1 µg/mL were tested on two different cell lines from human cervix carcinoma (HeLa) and human colorectal adenocarcinoma (DLD-)1 with colorimetric assay based on the MTT reagent (methy-134 thiazolyltetrazolium). The results show good luminescence spectra on all core-shell UCN. The MTT assays show that some concentrations of bare UCN of Y2O3: Er, Yb (1%, 1% mol) and Gd2O3 were cytotoxic for cervical adenocarcinoma cells (HeLa). For human colorectal adenocarcinoma all UCN are non-cytotoxic. The UCN with silica-aminosilane functionalization (APTS/TEOS) were non-cytotoxic on both cell lines.

Upconversion Nanoparticles Y2O3 and Gd2O3 Co-Doped with Er3+ and Yb3+ with Aminosilane-Folic Acid Functionalization for Breast and Cervix Cancer Cells Detection

MRS Advances ◽  
2017 ◽  
Vol 2 (52) ◽  
pp. 2983-2988 ◽  
Author(s):  
D. Chávez-García ◽  
K. Juárez-Moreno ◽  
G.A. Hirata
Keyword(s):  
Particle Size ◽  
Folic Acid ◽  
Cancer Cells ◽  
Near Infrared ◽  
Colorimetric Assay ◽  
Rare Earth Ions ◽  
Upconversion Nanoparticles ◽  
Breast Cancers ◽  
Transmission Electron ◽  
Cervical Cancer Cells

ABSTRACTThe upconversion nanoparticles (UCNPs) possess the ability to absorb near infrared energy (980 nm) and upconvert it to emit in the visible spectra. In this research, the UNCPs emit in red (660 nm) due to the electronic transitions between two rare earth ions: Er3+ and Yb3+, this process is called energy transfer upconversion (ETU). The UCNPs were functionalized with aminosilanes and folic acid receptors (UCNP-FR) and analyzed by transmission electron microscopy, Fourier transform infrared spectroscopy and luminescence measurements. UCNPs-FR of Y2O3 have a particle size of 70 ± 10 nm and the Gd2O3 have a 50 ± 10 nm particle size. Both showed a good luminescence spectrum in comparison with the bare ones. Cytotoxicity of different amounts between 0.001 µg/ml to 1 µg/ml of bare and functionalized UCNPs was measured with the colorimetric assay MTT in three cancer cell lines: human cervical adenocarcinoma (HeLa), human breast cancer cells MB-MDA-231. Some concentrations of bare UCNPs were cytotoxic for cancer cells; however after their functionalization they resulted to be non-cytotoxic. The functionalized UCNPs were able to bind to folate receptors which are overexpressed in cervical and breast cancers cells. It was demonstrated by confocal microscopy, that the functionalized UCNPs were internalized into the cancer cells, confirming that they can be used as biolabels for breast and cervical cancer cells.

scholarly journals Differential Effects of Gold Nanoparticles and Ionizing Radiation on Cell Motility between Primary Human Colonic and Melanocytic Cells and Their Cancerous Counterparts

2021 ◽  
Vol 22 (3) ◽  
pp. 1418
Author(s):  
Elham Shahhoseini ◽  
Masao Nakayama ◽  
Terrence J. Piva ◽  
Moshi Geso
Keyword(s):  
Gold Nanoparticles ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Cell Lines ◽  
Colorectal Adenocarcinoma ◽  
X Rays ◽  
Cell Adherence ◽  
Cancerous Cell ◽  
Primary Colon ◽  
Radiation Induced

This study examined the effects of gold nanoparticles (AuNPs) and/or ionizing radiation (IR) on the viability and motility of human primary colon epithelial (CCD841) and colorectal adenocarcinoma (SW48) cells as well as human primary epidermal melanocytes (HEM) and melanoma (MM418-C1) cells. AuNPs up to 4 mM had no effect on the viability of these cell lines. The viability of the cancer cells was ~60% following exposure to 5 Gy. Exposure to 5 Gy X-rays or 1 mM AuNPs showed the migration of the cancer cells ~85% that of untreated controls, while co-treatment with AuNPs and IR decreased migration to ~60%. In the non-cancerous cell lines gap closure was enhanced by ~15% following 1 mM AuNPs or 5 Gy treatment, while for co-treatment it was ~22% greater than that for the untreated controls. AuNPs had no effect on cell re-adhesion, while IR enhanced only the re-adhesion of the cancer cell lines but not their non-cancerous counterparts. The addition of AuNPs did not enhance cell adherence. This different reaction to AuNPs and IR in the cancer and normal cells can be attributed to radiation-induced adhesiveness and metabolic differences between tumour cells and their non-cancerous counterparts.

scholarly journals Functionalized Upconversion Nanoparticles for Targeted Labelling of Bladder Cancer Cells

Biomolecules ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 820 ◽  
Author(s):  
Dmitry Polikarpov ◽  
Liuen Liang ◽  
Andrew Care ◽  
Anwar Sunna ◽  
Douglas Campbell ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
Cancer Cells ◽  
Near Infrared ◽  
Carcinoma Cells ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Silica Layer ◽  
Bladder Cancer Cells

Bladder cancer is the ninth most common cancer worldwide. Due to a high risk of recurrence and progression of bladder cancer, every patient needs long-term surveillance, which includes regular cystoscopy, sometimes followed by a biopsy of suspicious lesions or resections of recurring tumours. This study addresses the development of novel biohybrid nanocomplexes representing upconversion nanoparticles (UCNP) coupled to antibodies for photoluminescent (PL) detection of bladder cancer cells. Carrying specific antibodies, these nanoconjugates selectively bind to urothelial carcinoma cells and make them visible by emitting visible PL upon excitation with deeply penetrating near-infrared light. UCNP were coated with a silica layer and linked to anti-Glypican-1 antibody MIL38 via silica-specific solid-binding peptide. Conjugates have been shown to specifically attach to urothelial carcinoma cells with high expression of Glypican-1. This result highlights the potential of produced conjugates and conjugation technology for further studies of their application in the tumour detection and fluorescence-guided resection.

scholarly journals Antitumor Activity and Immunomodulatory Effects of Ficus carica Latex

2021 ◽  
Author(s):  
Hamideh Azami ◽  
Saeed Malek-Hosseini ◽  
Maryam Mojahed Taghi ◽  
Mohammadrasul Zareinejad ◽  
Zahra Amirghofran
Keyword(s):  
Flow Cytometry ◽  
Cell Lines ◽  
Cancer Cell ◽  
Colorimetric Assay ◽  
Cancer Cell Lines ◽  
Proliferation Index ◽  
Cervix Carcinoma ◽  
Immunomodulatory Effects ◽  
Lymphoid Leukemia ◽  
Brdu Assay

Introduction: There are limited studies on the anti-cancer and immunomodulatory effects of the fig fruit latex. In this study, we aimed to investigate the effect of fig fruit latex on several cancer cell lines as well as its effect on lymphocytes proliferation and cytokines secretion. Methods: After preparing a methanolic extract from fig latex, its effect on various cancer cell lines including Fen (bladder cancer), K562 (myeloid leukemia), Hela (cervix carcinoma), Jurkat (lymphoid leukemia) and Raji (lymphoma) was examined by MTT colorimetric assay. For evaluating the effects of extract on lymphocyte proliferation and viability, BrdU assay and flow cytometry staining were used. Cytokine secretion was measured by ELISA assay. Results: The extract showed the strongest activity against K562 cell line (IC50, 234 µg/ml) and the least activity against Hela cells (IC50 >1000 µg/ml). On evaluation of the immunomodulatory effect of fig by BrdU assay, a reduction in lymphocytes proliferation by increasing the concentration of the extract was observed; proliferation index from 1.2±0.06 at 0.1 µg/ml of the extract reached to 0.13±0.2 at 800 µg/ml. In flow cytometry analysis, a significant cytotoxic effect at concentrations ≥400 µg/ml was observed. The extract at 100 and 200µg/ml had the ability to reduce secretion of interferon (IFN)-γ and interleukin (IL)-4 cytokines. Conclusion: Fig latex extract showed cytotoxicity on different cells particularly K562 leukemia cells which implied its anticancer activity. This extract at lower concentrations reduced lymphocytes proliferation and cytokine production which showed its immunoinhibitory effects and suggested its possible beneficial in treatment of immune-mediated diseases.

scholarly journals Wnt7a Promotes the Occurrence and Development of Colorectal Adenocarcinoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Congcong Li ◽  
Xiaowei Dou ◽  
Jiahuan Sun ◽  
Min Xie ◽  
Hongli Li ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Cancer Cells ◽  
Cell Lines ◽  
Colorectal Adenocarcinoma ◽  
Immunohistochemical Method ◽  
Regeneration Ability ◽  
Colorectal Mucosa ◽  
Colorectal Cancer Cells ◽  
Cloning Assay ◽  
Proliferation Ability

ObjectiveThe expression of Wnt7a in colorectal cancer tissues and cell lines was analyzed, and the effect of Wnt7a on the proliferation of colorectal cancer cells was studied, so as to confirm the relationship between Wnt7a and the occurrence and development of colorectal cancer.Methods(1) Immunohistochemical method was used to compare the expression of Wnt7a in different tissues and its relationship with the clinicopathology of colorectal adenocarcinoma. (2) The expression levels of Wnt7a in colorectal cancer cell lines HT-29 and HCT 116 were detected by qRT-PCR. (3) The down-regulated Wnt7A expression vector was constructed, and the down-regulated Wnt7A expression cell line was established. The regeneration ability of cancer cells was detected by stem cell ball formation assay, and the influence of plate cloning assay on the proliferation ability of colorectal cancer cells was detected.Results(1) The positive rates of Wnt7a in normal colorectal mucosa, colorectal adenoma and colorectal adenocarcinoma tissues gradually increased,Wnt7a are closely related to the degree of colorectal adenocarcinoma differentiation, lymph node metastasis and Duke stage. (2) The expression level of Wnt7a in colorectal cancer cells was higher than that in normal colorectal epithelial cells. (3) The down-regulation of Wnt7A reduced the proliferation ability of colorectal cancer cells.ConclusionsWnt7a promotes the occurrence and development of colorectal adenocarcinoma.

Sorafenib and 5-Fluorouracil Loaded Dual Drug Nanodelivery Systems for Hepatocellular Carcinoma and Colorectal Adenocarcinoma

2021 ◽  
Author(s):  
Umme Ruman ◽  
Kalaivani Buskaran ◽  
Saifullah Bullo ◽  
Georgia Pastorin ◽  
Mas Jaffri Masarudin ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cancer Cells ◽  
Cell Lines ◽  
Colorectal Adenocarcinoma ◽  
Dermal Fibroblast ◽  
Drug Loading ◽  
Chitosan Nanoparticles ◽  
Normal Human Dermal Fibroblast ◽  
Cytotoxicity Studies

Abstract Purpose: Here, we reported the sysnthesis of two clinically used drugs, 5-fluorouracil (5FU) and Sorafenib (SF)-loaded in chitosan nanoparticles and their priliminary study of therapeutics effect on hepatocellular carcinoma and colorectal adenocarcinoma cell lines. We have formulated chitosan nanoparticles (CS NPs) loaded dual (SF and 5-FU) drugs nanodelivery system for SF/5FU-CS NPs and their coating version with folic acid (FA) for SF/5FU-CS-FA NPS. Human hepatocellular carcinoma (HepG2) and colorectal adenocarcinoma (HT29) cell lines were selected for in vitro cytotoxicity studies to evaluate the preliminary anticancer efficacy of both nanoparticles.Characterization: The physiochemical characterization of SF/5FU-CS NPs and SF/5FU-CS-FA NPs were investigated by DLS, FESEM, HRTEM, EDX, XRD, TGA, FTIR, and HPLC methods.Results: DLS study has shown the size of SF/5FU-CS and SF/5FU-CS-FA nanoparticles were about 78±14 nm and 142±25 nm, respectively. HRTEM and FESEM studies confirmed the spherical shape with size of 60-70nm for SF/5FU-CS and 90-150 nm for SF/5FU-CS-FA NPs. The XRD results indicated the drug loading and folate-coating comfirmation. FTIR peaks confirmed the presence of drugs in the nanoparticles, as well as folate-coating on the surface of the nanoparticles. TGA results demonstrated the thermostability of both nanoparticles. The release profiles of SF and 5FU from the two designed NPs were found to be in a sustained manner according to the pseudo-second-order kinetics model indicating a good delivery system for tumor cells. The cytotoxicity studies confirmed the better anti-cancer activity of the nanoparticles compared to the free 5-fluorouracil and sorafenib against liver cancer cells, HepG2 and colon cancer cells, HT29. Conversely, both NPs were found not toxic towards normal human dermal fibroblast cells (HDF) cells.

Inhibition of PERK-dependent pro-adaptive signaling pathway as a promising approach for cancer treatment

2017 ◽  
Vol 89 (3) ◽  
pp. 7-10 ◽  
Author(s):  
Wioletta Rozpędek ◽  
Dariusz Pytel ◽  
Łukasz Dziki ◽  
Alicja Nowak ◽  
Adam Dziki ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Cell Lines ◽  
Small Molecule ◽  
Colorectal Adenocarcinoma ◽  
Initiation Factor ◽  
Human Neuroblastoma ◽  
Ht 29

Endoplasmic Reticulum (ER) is an organelle that is vital for cell growth and maintenance of homeostasis. Recent studies have reported that numerous human diseases, including cancer, are strictly connected to disruption of ER homeostasis. In order to counteract adverse intracellular conditions, cancer cells induce protein kinase R (PKR)-like endoplasmic reticulum kinase (PERK)-dependent, pro-adaptive unfolded protein response (UPR) signaling branches. If ER stress is severe or prolonged, pro-adaptive signaling networks are insufficient, resulting in apoptotic cell death of cancer cells. The main aim: of the study was to evaluate the biological activity of a small-molecule PERK inhibitor GSK2606414 in two cancer cell lines - human neuroblastoma (SH-SY5Y) and human colorectal adenocarcinoma (HT-29) cell lines. We analyzed the level of phosphorylation of the eukaryotic initiation factor 2 (eIF2), which is the main substrate of PERK and a subsequent activator of UPR, which under long-term ER stress may evoke apoptotic death of cancer cells. Material and methods: In the study, we utilized commercially available cell lines of human colorectal adenocarcinoma HT-29 and human neuroblastoma SH-SY5Y. Cells were exposed to the tested PERK-dependent signaling inhibitor GSK2606414 in suitable culture media with addition of thapsigargin (500 nM) to induce ER stress. To identify the protein, Western blot with specific antibodies was used. Detection of immune complexes was performed using chemiluminescence. Results: We found a complete inhibition of p-eIF2α expression due to the GSK2606414 inhibitor in both cell lines, SH-SY5Y and HT-29. Conclusions: Currently available cancer treatments are insufficient and cause various side effects. It has been assumed that utilization of small-molecule inhibitors of the PERK-dependent signaling pathway, like GSK2606414, may switch the pro-adaptive branch of UPR to its pro-apoptotic branch. It is believed that the tested inhibitor GSK2606414 may become a promising treatment for many cancer types.

scholarly journals Encapsulation of Upconversion Nanoparticles in Periodic Mesoporous Organosilicas

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4054 ◽  
Author(s):  
Saher Rahmani ◽  
Chiara Mauriello Jimenez ◽  
Dina Aggad ◽  
Daniel González-Mancebo ◽  
Manuel Ocaña ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Near Infrared ◽  
Sol Gel ◽  
Upconversion Nanoparticles ◽  
X Ray ◽  
Mesoporous Organosilica ◽  
The Matrix ◽  
X Ray Computed ◽  
Mesoporous Organosilicas ◽  
Cancer Theranostics

(1) Background: Nanomedicine has recently emerged as a promising field, particularly for cancer theranostics. In this context, nanoparticles designed for imaging and therapeutic applications are of interest. We, therefore, studied the encapsulation of upconverting nanoparticles in mesoporous organosilica nanoparticles. Indeed, mesoporous organosilica nanoparticles have been shown to be very efficient for drug delivery, and upconverting nanoparticles are interesting for near-infrared and X-ray computed tomography imaging, depending on the matrix used. (2) Methods: Two different upconverting-based nanoparticles were synthesized with Yb3+-Er3+ as the upconverting system and NaYF4 or BaLuF5 as the matrix. The encapsulation of these nanoparticles was studied through the sol-gel procedure with bis(triethoxysilyl)ethylene and bis(triethoxysilyl)ethane in the presence of CTAB. (3) Results: with bis(triethoxysilyl)ethylene, BaLuF5: Yb3+-Er3+, nanoparticles were not encapsulated, but anchored on the surface of the obtained mesoporous nanorods BaLuF5: Yb3+-Er3+@Ethylene. With bis(triethoxysilyl)ethane, BaLuF5: Yb3+-Er3+ and NaYF4: Yb3+-Er3+nanoparticles were encapsulated in the mesoporous cubic structure leading to BaLuF5: Yb3+-Er3+@Ethane and NaYF4: Yb3+-Er3+@Ethane, respectively. (4) Conclusions: upconversion nanoparticles were located on the surface of mesoporous nanorods obtained by hydrolysis polycondensation of bis(triethoxysilyl)ethylene, whereas encapsulation occurred with bis(triethoxysilyl)ethane. The later nanoparticles NaYF4: Yb3+-Er3+@Ethane or BaLuF5: Yb3+-Er3+@Ethane were promising for applications with cancer cell imaging or X-ray-computed tomography respectively.

Amphiphilic copolymer coated upconversion nanoparticles for near-infrared light-triggered dual anticancer treatment

Nanoscale ◽  
2014 ◽  
Vol 6 (24) ◽  
pp. 14903-14910 ◽  
Author(s):  
Shun Yang ◽  
Najun Li ◽  
Zhuang Liu ◽  
Wenwei Sha ◽  
Dongyun Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Controlled Release ◽  
Cancer Cells ◽  
Self Assembly ◽  
Near Infrared ◽  
Amphiphilic Copolymer ◽  
Infrared Light ◽  
Upconversion Nanoparticles ◽  
Assembly Process ◽  
Anticancer Treatment

The light-triggered controlled release of anticancer drugs accompanied with NIR-responsive photodynamic therapy was performed via a self-assembly process. Under 980 nm laser, the cancer cells could be effectively inhibited by released drugs and singlet oxygen simultaneously.

Sign in / Sign up

Export Citation Format

Share Document