scholarly journals Nucleic Acids Extraction from Formalin-Fixed and Paraffin-Embedded Tissues

10.5772/61581 ◽  
2016 ◽  
Author(s):  
Gisele R. Gouveia ◽  
Suzete C. Ferreira ◽  
Sheila A. C. Siqueira ◽  
Juliana Pereira
Keyword(s):  
Nucleic Acids ◽  
Formalin Fixed

scholarly journals A MICROPHOTOMETRIC STUDY OF THE SYNTHESES OF DESOXYRIBONUCLEIC ACID AND NUCLEAR HISTONE

1955 ◽  
Vol 1 (1) ◽  
pp. 17-28 ◽  
Author(s):  
David P. Bloch ◽  
Gabriel C. Godman
Keyword(s):  
Cell Division ◽  
Nucleic Acids ◽  
Standard Error ◽  
Basic Protein ◽  
Desoxyribonucleic Acid ◽  
Fast Green ◽  
Nuclear Histone ◽  
Source Of Error ◽  
Formalin Fixed

1. The fast green stain of Alfert and Geschwind for nuclear basic protein is shown to obey the Beer-Lambert laws when used on purified histone. Interference from acid substances other than nucleic acids as a possible source of error is indicated. 2. Use of this technique after a modified Feulgen stain enables determination of relative amounts of desoxyribonucleic acid and histone in the same individual cells. 3. DNA and histone are shown to have the same distribution in formalin-fixed nuclei. 4. The syntheses of DNA and histone proceed simultaneously resulting in the doubling of both these substances prior to cell division. 5. The standard error for histone values is greater than that for DNA; however, the source of this variability is not known.

scholarly journals Coextraction and PCR Based Analysis of Nucleic Acids From Formalin-Fixed Paraffin-Embedded Specimens

2014 ◽  
Vol 29 (6) ◽  
pp. 485-492 ◽  
Author(s):  
Souvik Ghatak ◽  
Zothan sanga ◽  
Jeremy L. Pautu ◽  
Nachimuthu Senthil Kumar
Keyword(s):  
Nucleic Acids ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples

2012 ◽  
Vol 56 (9) ◽  
pp. 618-626 ◽  
Author(s):  
Marina M. L. Kizys ◽  
Mirian G. Cardoso ◽  
Susan C. Lindsey ◽  
Michelle Y. Harada ◽  
Fernando A. Soares ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Proteinase K ◽  
Acid Extraction ◽  
Salting Out ◽  
Fine Needle ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26

Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

2010 ◽  
Vol 400 (1) ◽  
pp. 110-117 ◽  
Author(s):  
John B.A. Okello ◽  
Jaymi Zurek ◽  
Alison M. Devault ◽  
Melanie Kuch ◽  
Andrew L. Okwi ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Comparison Of Methods ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Autopsy Tissues ◽  
Formalin Fixed

scholarly journals Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Jessica Carlsson ◽  
Sabina Davidsson ◽  
Jonna Fridfeldt ◽  
Francesca Giunchi ◽  
Valentina Fiano ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Prostate Biopsies ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Tissue Handling and Specimen Preparation in Surgical Pathology: Issues Concerning the Recovery of Nucleic Acids From Formalin-Fixed, Paraffin-Embedded Tissue

2008 ◽  
Vol 132 (12) ◽  
pp. 1929-1935 ◽  
Author(s):  
Stephen M. Hewitt ◽  
Fraser A. Lewis ◽  
Yanxiang Cao ◽  
Richard C. Conrad ◽  
Maureen Cronin ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Expression Profiling ◽  
Additional Research ◽  
Specimen Preparation ◽  
Academic Leaders ◽  
Tissue Handling ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

Abstract Context.—Expression profiling by microarrays and real-time polymerase chain reaction–based assays is a powerful tool for classification and prognostication of disease; however, it remains a research tool, largely reliant on frozen tissue. Limiting the utility of expression profiling is the isolation of quality nucleic acids from formalin-fixed, paraffin-embedded tissue. The collection, handling, and processing of tissue directly impacts the biomolecules that can be recovered from it. High-quality nucleic acids can be obtained from formalin-fixed, paraffin-embedded tissue, but greater attention to all steps in the process of tissue handling and preparation is required. Objective.—To summarize the current state-of-the-art of preanalytic factors in tissue handling and processing as they impact the quality of RNA obtainable from formalin-fixed, paraffin-embedded tissue. The goals are to provide recommendations that will improve RNA quality for expression profiling from formalin-fixed, paraffin-embedded tissue and highlight areas for additional research. Tissue is an analyte and it must be handled in a standardized fashion to provide consistent results. Data Sources.—The literature was reviewed. Consultation with industry and academic leaders in the use of RNA for expression profiling was obtained to identify areas for additional research. Conclusions.—Development of RNA-based assays from formalin-fixed, paraffin-embedded tissue is feasible. Greater attention to tissue handing and processing is essential to improve the quality of biospecimens for the development of robust RNA-based assays. Standardization of procedures and vigorous testing of alternative protocols are required to ensure that these assays function as designed.

Ultrastructural Localization of Acid Mucosubstance in Skeletal Muscle

1967 ◽  
Vol 25 ◽  
pp. 52-53 ◽  
Author(s):  
William J. Dougherty ◽  
Samuel S. Spicer
Keyword(s):  
Striated Muscle ◽  
Divalent Cations ◽  
Ultrastructural Localization ◽  
Major Elements ◽  
Frozen Sections ◽  
Thorium Dioxide ◽  
Iron Solution ◽  
Coupling System ◽  
Psoas Muscles ◽  
Formalin Fixed

In recent years, considerable attention has focused on the morphological nature of the excitation-contraction coupling system of striated muscle. Since the study of Porter and Palade, it has become evident that the sarcoplastic reticulum (SR) and transverse tubules constitute the major elements of this system. The problem still exists, however, of determining the mechamisms by which the signal to interdigitate is presented to the thick and thin myofilaments. This problem appears to center on the movement of Ca++ions between myofilaments and SR. Recently, Philpott and Goldstein reported acid mucosubstance associated with the SR of fish branchial muscle using the colloidal thorium dioxide technique, and suggested that this material may serve to bind or release divalent cations such as Ca++. In the present study, Hale's iron solution adapted to electron microscopy was applied to formalin-fixed myofibrils isolated from glycerol-extracted rabbit psoas muscles and to frozen sections of formalin-fixed rat psoas muscles.

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