Comparison of methods in the recovery of nucleic acids from archival formalin-fixed paraffin-embedded autopsy tissues

2010 ◽  
Vol 400 (1) ◽  
pp. 110-117 ◽  
Author(s):  
John B.A. Okello ◽  
Jaymi Zurek ◽  
Alison M. Devault ◽  
Melanie Kuch ◽  
Andrew L. Okwi ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Comparison Of Methods ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Autopsy Tissues ◽  
Formalin Fixed

scholarly journals Coextraction and PCR Based Analysis of Nucleic Acids From Formalin-Fixed Paraffin-Embedded Specimens

2014 ◽  
Vol 29 (6) ◽  
pp. 485-492 ◽  
Author(s):  
Souvik Ghatak ◽  
Zothan sanga ◽  
Jeremy L. Pautu ◽  
Nachimuthu Senthil Kumar
Keyword(s):  
Nucleic Acids ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Optimizing nucleic acid extraction from thyroid fine-needle aspiration cells in stained slides, formalin-fixed/paraffin-embedded tissues, and long-term stored blood samples

2012 ◽  
Vol 56 (9) ◽  
pp. 618-626 ◽  
Author(s):  
Marina M. L. Kizys ◽  
Mirian G. Cardoso ◽  
Susan C. Lindsey ◽  
Michelle Y. Harada ◽  
Fernando A. Soares ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Proteinase K ◽  
Acid Extraction ◽  
Salting Out ◽  
Fine Needle ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

OBJECTIVE: Adequate isolation of nucleic acids from peripheral blood, fine-needle aspiration cells in stained slides, and fresh and formalin-fixed/paraffin-embedded tissues is crucial to ensure the success of molecular endocrinology techniques, especially when samples are stored for long periods, or when no other samples can be collected from patients who are lost to follow-up. Here, we evaluate several procedures to improve current methodologies for DNA (salting-out) and RNA isolation. MATERIALS AND METHODS: We used proteinase K treatment, heat shock, and other adaptations to increase the amount and quality of the material retrieved from the samples. RESULTS: We successfully isolated DNA and RNA from the samples described above, and this material was suitable for PCR, methylation profiling, real-time PCR and DNA sequencing. CONCLUSION: The techniques herein applied to isolate nucleic acids allowed further reliable molecular analyses. Arq Bras Endocrinol Metab. 2012;56(9):618-26

Genotyping of Toxoplasma gondii: DNA extraction from formalin-fixed paraffin-embedded autopsy tissues from AIDS patients who died by severe disseminated toxoplasmosis

2016 ◽  
Vol 165 ◽  
pp. 16-21 ◽  
Author(s):  
Inara Bastos da Silva ◽  
Tatiana Pimental de Andrade Batista ◽  
Roosecelis Brasil Martines ◽  
Cristina Takami Kanamura ◽  
Isabelle Martins Ribeiro Ferreira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Dna Extraction ◽  
Aids Patients ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Autopsy Tissues ◽  
Formalin Fixed

scholarly journals Quantity and quality of nucleic acids extracted from archival formalin fixed paraffin embedded prostate biopsies

2018 ◽  
Vol 18 (1) ◽  
Author(s):  
Jessica Carlsson ◽  
Sabina Davidsson ◽  
Jonna Fridfeldt ◽  
Francesca Giunchi ◽  
Valentina Fiano ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Prostate Biopsies ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
Sign in / Sign up

Export Citation Format

Share Document