scholarly journals Regenerative Medicine for Neurological Diseases with the Use of Electrical Stimulation

10.5772/55612 ◽  
2013 ◽  
Author(s):  
Masahiro Kameda
Keyword(s):  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Neurological Diseases

scholarly journals Modulation of Human Mesenchymal Stem Cells by Electrical Stimulation Using an Enzymatic Biofuel Cell

Catalysts ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 62
Author(s):  
Won-Yong Jeon ◽  
Seyoung Mun ◽  
Wei Beng Ng ◽  
Keunsoo Kang ◽  
Kyudong Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Cell Morphology ◽  
Electrical Current ◽  
Specific Gene ◽  
Next Generation ◽  
The Impact

Enzymatic biofuel cells (EBFCs) have excellent potential as components in bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. We tested three different electrical currents, 127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). We also observed changes in cytoskeleton arrangement and analyzed gene expression that depends on the electrical stimulation. The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. In conclusion, this study demonstrates that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells; such capabilities can be useful for regenerative medicine applications such as bioelectronic devices.

Regenerative medicine for neurological diseases—will regenerative neurosurgery deliver?

BMJ ◽  
2021 ◽  
pp. n955
Author(s):  
Terry C Burns ◽  
Alfredo Quinones-Hinojosa
Keyword(s):  
Regenerative Medicine ◽  
Neurological Diseases ◽  
Cell Types ◽  
Ethical Challenges ◽  
Cord Injury ◽  
Practice Of Medicine ◽  
Challenges And Opportunities ◽  
The Central Nervous System ◽  
Future Practice ◽  
Lateral Sclerosis

Abstract Regenerative medicine aspires to transform the future practice of medicine by providing curative, rather than palliative, treatments. Healing the central nervous system (CNS) remains among regenerative medicine’s most highly prized but formidable challenges. “Regenerative neurosurgery” provides access to the CNS or its surrounding structures to preserve or restore neurological function. Pioneering efforts over the past three decades have introduced cells, neurotrophins, and genes with putative regenerative capacity into the CNS to combat neurodegenerative, ischemic, and traumatic diseases. In this review we critically evaluate the rationale, paradigms, and translational progress of regenerative neurosurgery, harnessing access to the CNS to protect, rejuvenate, or replace cell types otherwise irreversibly compromised by neurological disease. We discuss the evidence surrounding fetal, somatic, and pluripotent stem cell derived implants to replace endogenous neuronal and glial cell types and provide trophic support. Neurotrophin based strategies via infusions and gene therapy highlight the motivation to preserve neuronal circuits, the complex fidelity of which cannot be readily recreated. We specifically highlight ongoing translational efforts in Parkinson’s disease, amyotrophic lateral sclerosis, stroke, and spinal cord injury, using these to illustrate the principles, challenges, and opportunities of regenerative neurosurgery. Risks of associated procedures and novel neurosurgical trials are discussed, together with the ethical challenges they pose. After decades of efforts to develop and refine necessary tools and methodologies, regenerative neurosurgery is well positioned to advance treatments for refractory neurological diseases. Strategic multidisciplinary efforts will be critical to harness complementary technologies and maximize mechanistic feedback, accelerating iterative progress toward cures for neurological diseases.

scholarly journals Modulation of human mesenchymal stem cells by electrical stimulation using an enzymatic biofuel cell

2020 ◽  
Author(s):  
Won-Yong Jeon ◽  
Seyoung Mun ◽  
WeiBeng Ng ◽  
Keunsoo Kang ◽  
Kyudong Han ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Cell Morphology ◽  
Wearable Sensors ◽  
Electrical Current ◽  
Next Generation ◽  
The Impact

Abstract Background Enzymatic biofuel cells (EBFCs) have excellent potential as components in wearable sensors and bioelectronic devices, especially as active biointerfaces to regulate stem cell behavior for regenerative medicine applications. However, it remains unclear to what extent EBFC-generated electrical stimulation can regulate the functional behavior of human adipose-derived mesenchymal stem cells (hAD-MSCs) at the morphological and gene expression levels. Herein, we investigated the effect of EBFC-generated electrical stimulation on hAD-MSC cell morphology and gene expression using next-generation RNA sequencing. Materials We tested three different electrical currents,127 ± 9, 248 ± 15, and 598 ± 75 nA/cm2, in mesenchymal stem cells. We performed transcriptome profiling to analyze the impact of EBFC-derived electrical current on gene expression using next generation sequencing (NGS). Also we observed changes in cytoskeleton arrangement and analyzed gene expression depends on the electrical stimulation. Results The electrical stimulation of EBFC changes cell morphology through cytoskeleton re-arrangement. In particular, the results of whole transcriptome NGS showed that specific gene clusters were up- or down-regulated depending on the magnitude of applied electrical current of EBFC. Conclusion Taken together, the findings in this study demonstrate that EBFC-generated electrical stimulation can influence the morphological and gene expression properties of stem cells and such capabilities can be useful for regenerative medicine applications related to wearable sensors and devices.

scholarly journals A Novel Vitronectin Peptide Facilitates Differentiation of Oligodendrocytes from Human Pluripotent Stem Cells (Synthetic ECM for Oligodendrocyte Differentiation)

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1254
Author(s):  
Won Ung Park ◽  
Gyu-Bum Yeon ◽  
Myeong-Sang Yu ◽  
Hui-Gwan Goo ◽  
Su-Hee Hwang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Pluripotent Stem Cells ◽  
Neurological Diseases ◽  
Docking Simulation ◽  
Human Pluripotent Stem Cells ◽  
Oligodendrocyte Differentiation ◽  
In Silico Docking ◽  
Cytoskeleton Remodeling ◽  
Culture Environment

Differentiation of oligodendrocytes (ODs) presents a challenge in regenerative medicine due to their role in various neurological diseases associated with dysmyelination and demyelination. Here, we designed a peptide derived from vitronectin (VN) using in silico docking simulation and examined its use as a synthetic substrate to support the differentiation of ODs derived from human pluripotent stem cells. The designed peptide, named VNP2, promoted OD differentiation induced by the overexpression of SOX10 in OD precursor cells compared with Matrigel and full-length VN. ODs differentiated on VNP2 exhibited greater contact with axon-mimicking nanofibers than those differentiated on Matrigel. Transcriptomic analysis revealed that the genes associated with morphogenesis, cytoskeleton remodeling, and OD differentiation were upregulated in cells grown on VNP2 compared with cells grown on Matrigel. This new synthetic VN-derived peptide can be used to develop a culture environment for efficient OD differentiation.

Nanomaterials in Neural-Stem-Cell-Mediated Regenerative Medicine: Imaging and Treatment of Neurological Diseases

2018 ◽  
Vol 30 (17) ◽  
pp. 1705694 ◽  
Author(s):  
Bingbo Zhang ◽  
Wei Yan ◽  
Yanjing Zhu ◽  
Weitao Yang ◽  
Wenjun Le ◽  
...  
Keyword(s):  
Stem Cell ◽  
Regenerative Medicine ◽  
Neural Stem Cell ◽  
Neurological Diseases

A Review of the Impact of Electrical Stimulation on the Stem Cells Fate and Its Application in Regenerative Medicine and Cancer Treatment

2020 ◽  
Vol 11 (2) ◽  
pp. 139-153
Author(s):  
AR Farmani ◽  
M Mohammad Salehi ◽  
F Mahdavinezhad ◽  
M Kouhestani ◽  
S Mohammadi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Cancer Treatment ◽  
The Impact

scholarly journals Neuronal Reprogramming for Tissue Repair and Neuroregeneration

2020 ◽  
Vol 21 (12) ◽  
pp. 4273 ◽  
Author(s):  
Roxanne Hsiang-Chi Liou ◽  
Thomas L. Edwards ◽  
Keith R. Martin ◽  
Raymond Ching-Bong Wong
Keyword(s):  
Regenerative Medicine ◽  
Neurological Diseases ◽  
Neuronal Cell ◽  
Cell Types ◽  
Cellular Reprogramming ◽  
Future Directions ◽  
Promising Target ◽  
Retinal Degenerative Diseases

Stem cell and cell reprogramming technology represent a rapidly growing field in regenerative medicine. A number of novel neural reprogramming methods have been established, using pluripotent stem cells (PSCs) or direct reprogramming, to efficiently derive specific neuronal cell types for therapeutic applications. Both in vitro and in vivo cellular reprogramming provide diverse therapeutic pathways for modeling neurological diseases and injury repair. In particular, the retina has emerged as a promising target for clinical application of regenerative medicine. Herein, we review the potential of neuronal reprogramming to develop regenerative strategy, with a particular focus on treating retinal degenerative diseases and discuss future directions and challenges in the field.

scholarly journals Controlling properties of human neural progenitor cells using 2D and 3D conductive polymer scaffolds

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Shang Song ◽  
Danielle Amores ◽  
Cheng Chen ◽  
Kelly McConnell ◽  
Byeongtaek Oh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Progenitor Cells ◽  
Neural Progenitor Cells ◽  
Neurological Diseases ◽  
Conductive Polymer ◽  
Physical Nature ◽  
Neural Progenitor ◽  
2D And 3D

AbstractHuman induced pluripotent stem cell-derived neural progenitor cells (hNPCs) are a promising cell source for stem cell transplantation to treat neurological diseases such as stroke and peripheral nerve injuries. However, there have been limited studies investigating how the dimensionality of the physical and electrical microenvironment affects hNPC function. In this study, we report the fabrication of two- and three-dimensional (2D and 3D respectively) constructs composed of a conductive polymer to compare the effect of electrical stimulation of hydrogel-immobilized hNPCs. The physical dimension (2D vs 3D) of stimulating platforms alone changed the hNPCs gene expression related to cell proliferation and metabolic pathways. The addition of electrical stimulation was critical in upregulating gene expression of neurotrophic factors that are important in regulating cell survival, synaptic remodeling, and nerve regeneration. This study demonstrates that the applied electrical field controls hNPC properties depending on the physical nature of stimulating platforms and cellular metabolic states. The ability to control hNPC functions can be beneficial in understanding mechanistic changes related to electrical modulation and devising novel treatment methods for neurological diseases.

Effects of Electrical Stimulation on Stem Cells

2020 ◽  
Vol 15 (5) ◽  
pp. 441-448 ◽  
Author(s):  
Wang Heng ◽  
Mit Bhavsar ◽  
Zhihua Han ◽  
John H. Barker
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Regenerative Medicine ◽  
Cell Function ◽  
Recent Interest ◽  
Scaffold Materials ◽  
Block Cell Proliferation

Recent interest in developing new regenerative medicine- and tissue engineering-based treatments has motivated researchers to develop strategies for manipulating stem cells to optimize outcomes in these potentially, game-changing treatments. Cells communicate with each other, and with their surrounding tissues and organs via electrochemical signals. These signals originate from ions passing back and forth through cell membranes and play a key role in regulating cell function during embryonic development, healing, and regeneration. To study the effects of electrical signals on cell function, investigators have exposed cells to exogenous electrical stimulation and have been able to increase, decrease and entirely block cell proliferation, differentiation, migration, alignment, and adherence to scaffold materials. In this review, we discuss research focused on the use of electrical stimulation to manipulate stem cell function with a focus on its incorporation in tissue engineering-based treatments.

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