scholarly journals Detection and Quantitation of Olive Pollen Allergen Isoforms Using 2-D Western Blotting

10.5772/46171 ◽  
2012 ◽  
Author(s):  
Krzysztof Zienkiewicz ◽  
Estefania Garcia-Quiros ◽  
Juan de Dios Alche ◽  
Maria Isabel ◽  
Antonio Jesus
Keyword(s):  
Western Blotting ◽  
Pollen Allergen ◽  
Olive Pollen

A sensitive monoclonal antibody ELISA for quantitication of the major olive pollen allergen Ole e 1: Applications and comparison with alternative methods

2002 ◽  
Vol 109 (1) ◽  
pp. S329-S329
Author(s):  
Juan A Asturias ◽  
Carmen Arilla ◽  
Ignacio Ibarrola ◽  
Elena Eraso ◽  
Jaime Algorta ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Pollen Allergen ◽  
Alternative Methods ◽  
Antibody Elisa ◽  
Olive Pollen

A Sensitive Monoclonal Antibody Sandwich ELISA for the Measurement of the Major Olive Pollen Allergen Ole e 1

2000 ◽  
Vol 122 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Ronald van Ree ◽  
Marja Aalbers ◽  
Olga Kea ◽  
Francisco M. Marco de la Calle ◽  
José M. Sempere Ortells ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sandwich Elisa ◽  
Pollen Allergen ◽  
Olive Pollen

The major olive pollen allergen (Ole e I) shows both gametophytic and sporophytic expression during anther development, and its synthesis and storage takes place in the RER

1999 ◽  
Vol 112 (15) ◽  
pp. 2501-2509 ◽  
Author(s):  
J. de Dios Alche ◽  
A.J. Castro ◽  
A. Olmedilla ◽  
M.C. Fernandez ◽  
R. Rodriguez ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
In Situ Hybridization ◽  
Membrane System ◽  
Polymerase Chain Reaction Analysis ◽  
Pollen Allergen ◽  
Transcriptional Level ◽  
Tetrad Stage ◽  
Olive Pollen ◽  
And Storage

The distribution of Ole e I (the major olive pollen allergen) and its transcripts was investigated in the anther from premeiotic stages until the dehiscent pollen stage. Crude protein extracts were analyzed by immunoblotting and probed with a monoclonal antibody to Ole e I. The protein, with three variants, was found to accumulate from the early microspore stage onwards. In addition to the previously reported localization of the protein, Ole e I has been immunolocalized for the first time within the pollen wall and in the tapetum. Reverse transcription-polymerase chain reaction analysis using specific oligonucleotides and RNA extracted from whole anthers revealed that the Ole e I gene is expressed from the late tetrad stage onwards. No expression was found in control tissues such as petals, roots or leaves. Light microscopy in situ hybridization on developing flower buds and dehiscent pollen confirmed the transcripts to be present in both the microspores and the sporophytic tissue (tapetum). Labeling was found primarily in the tapetum, reaching the highest concentration in the cytoplasm of the developing and mature pollen, once tapetum started to degenerate. In situ hybridization at the transmission electron microscope level showed the transcripts to accumulate on ribosomes of the rough endoplasmic reticulum. These studies, together with others carried out previously by us, indicated that both synthesis and storage of Ole e I take place in the endoplasmic reticulum, coincidentally with the conspicuous changes suffered by this membrane system during pollen development. This process is most likely controlled at the transcriptional level. The localization of the protein in the pollen ectexine bring new insights into the function of the allergen, which are discussed.

scholarly journals Ole e 9, a Major Olive Pollen Allergen Is a 1,3-β-Glucanase

2001 ◽  
Vol 276 (30) ◽  
pp. 27959-27966 ◽  
Author(s):  
Sonia Huecas ◽  
Mayte Villalba ◽  
Rosalı́a Rodrı́guez
Keyword(s):  
Pollen Allergen ◽  
Olive Pollen

Olive pollen allergen Ole e 8: identification in mature pollen and presence of Ole e 8-like proteins in different pollens

Allergy ◽  
2002 ◽  
Vol 57 (1) ◽  
pp. 40-43 ◽  
Author(s):  
A. Ledesma ◽  
M. Villalba ◽  
F. Vivanco ◽  
R. Rodriguez
Keyword(s):  
Pollen Allergen ◽  
Mature Pollen ◽  
Olive Pollen

A recombinant functional variant of the olive pollen allergen Ole e 10 expressed in baculovirus system

2006 ◽  
Vol 121 (3) ◽  
pp. 402-409 ◽  
Author(s):  
Patricia Barral ◽  
Alicia G. Serrano ◽  
Eva Batanero ◽  
Jesús Pérez-Gil ◽  
Mayte Villalba ◽  
...  
Keyword(s):  
Pollen Allergen ◽  
Functional Variant ◽  
Baculovirus System ◽  
Olive Pollen

Identification of a 36‐kDa olive‐pollen allergen by in vitro and in vivo studies

Allergy ◽  
1999 ◽  
Vol 54 (6) ◽  
pp. 584-592 ◽  
Author(s):  
A Martínez ◽  
JA Asturias ◽  
R Palacios ◽  
J Martínez ◽  
Ml Sanz ◽  
...  
Keyword(s):  
Pollen Allergen ◽  
In Vivo Studies ◽  
Olive Pollen

scholarly journals EXPRESSION OF OLE E 1, THE MAJOR OLIVE POLLEN ALLERGEN, DURING IN-VITRO POLLEN GERMINATION

2002 ◽  
pp. 465-468 ◽  
Author(s):  
M. M'rani-Alaoui ◽  
A.J. Castro ◽  
J.D. Alché ◽  
W. Wang ◽  
M.C. Fernández ◽  
...  
Keyword(s):  
Pollen Germination ◽  
Pollen Allergen ◽  
In Vitro Pollen Germination ◽  
Olive Pollen
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