A Sensitive Monoclonal Antibody Sandwich ELISA for the Measurement of the Major Olive Pollen Allergen Ole e 1

2000 ◽  
Vol 122 (3) ◽  
pp. 224-228 ◽  
Author(s):  
Ronald van Ree ◽  
Marja Aalbers ◽  
Olga Kea ◽  
Francisco M. Marco de la Calle ◽  
José M. Sempere Ortells ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sandwich Elisa ◽  
Pollen Allergen ◽  
Olive Pollen

A sensitive monoclonal antibody ELISA for quantitication of the major olive pollen allergen Ole e 1: Applications and comparison with alternative methods

2002 ◽  
Vol 109 (1) ◽  
pp. S329-S329
Author(s):  
Juan A Asturias ◽  
Carmen Arilla ◽  
Ignacio Ibarrola ◽  
Elena Eraso ◽  
Jaime Algorta ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Pollen Allergen ◽  
Alternative Methods ◽  
Antibody Elisa ◽  
Olive Pollen

Lactate Dehydrogenase Monoclonal Antibody Sandwich ELISA To Determine Cooking Temperature of Ground Beef

1996 ◽  
Vol 44 (12) ◽  
pp. 4048-4051 ◽  
Author(s):  
A. Orta-Ramirez ◽  
C. H. Wang ◽  
M. M. Abouzied ◽  
G. J. Veeramuthu ◽  
J. F. Price ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Lactate Dehydrogenase ◽  
Sandwich Elisa ◽  
Ground Beef ◽  
Cooking Temperature

scholarly journals Detection of Mycoplasma agalactiae Antigen in Sheep and Goats by Monoclonal Antibody-Based Sandwich ELISA

2004 ◽  
Vol 73 (4) ◽  
pp. 461-464 ◽  
Author(s):  
D. Zendulková ◽  
H. J. Ball ◽  
A. Madanat ◽  
P. Lány ◽  
Z. Pospíšil
Keyword(s):  
Monoclonal Antibody ◽  
Sandwich Elisa ◽  
Mycoplasma Agalactiae ◽  
Sheep And Goats

Development of a monoclonal antibody against the left wing of ciguatoxin CTX1B: Thiol strategy and detection using a sandwich ELISA

Toxicon ◽  
2012 ◽  
Vol 60 (3) ◽  
pp. 348-357 ◽  
Author(s):  
Takeshi Tsumuraya ◽  
Katsutoshi Takeuchi ◽  
Shuji Yamashita ◽  
Ikuo Fujii ◽  
Masahiro Hirama
Keyword(s):  
Monoclonal Antibody ◽  
Sandwich Elisa ◽  
Left Wing

A reverse-sandwich ELISA for IgG antibody to a pollen allergen

1988 ◽  
Vol 82 (3) ◽  
pp. 407-412 ◽  
Author(s):  
Hiroshi Miyazawa ◽  
Sakae Inouye ◽  
Masahiro Sakaguchi ◽  
Kazuhiro Koizumi
Keyword(s):  
Sandwich Elisa ◽  
Pollen Allergen ◽  
Igg Antibody

scholarly journals PRRSV Vaccine Strain-Induced Secretion of Extracellular ISG15 Stimulates Porcine Alveolar Macrophage Antiviral Response against PRRSV

Viruses ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1009
Author(s):  
Hongbin Liu ◽  
Bingjun Shi ◽  
Zhigang Zhang ◽  
Bao Zhao ◽  
Guangming Zhao ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Vaccine Strain ◽  
Sandwich Elisa ◽  
Respiratory Syndrome Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Porcine Alveolar Macrophage ◽  
Type I ◽  
Swine Industry ◽  
Host Interactions ◽  
Isg15 Expression

Porcine reproductive and respiratory syndrome virus (PRRSV) has disrupted the global swine industry since the 1980s. PRRSV-host interactions are largely still unknown but may involve host ISG15 protein. In this study, we developed a monoclonal antibody (Mab-3D5E6) specific for swine ISG15 (sISG15) by immunizing mice with recombinant sISG15. A sandwich enzyme-linked immunosorbent assay (ELISA) incorporating this sISG15-specific Mab was developed to detect sISG15 and provided a lower limit of sISG15 detection of 200 pg/mL. ELISA results demonstrated that infection of porcine alveolar macrophages (PAMs) with low-virulence or attenuated PRRSV vaccine strains induced intracellular ISG15 expression that was independent of type I IFN production, while PAMs infection with a PRRSV vaccine strain promoted extracellular ISG15 secretion from infected PAMs. Conversely, the addition of recombinant sISG15 to PAMs mimicked natural extracellular ISG15 effects whereby sISG15 functioned as a cytokine by activating PAMs. Once activated, PAMs could inhibit PRRSV replication and resist infection with PRRSV vaccine strain TJM. In summary, a sandwich ELISA incorporating homemade anti-ISG15 Mab detected ISG15 secretion induced by PAMs infection with a PRRSV vaccine strain. Recombinant ISG15 added to cells exhibited cytokine-like activity that stimulated PAMs to assume an anti-viral state that enabled them to inhibit PRRSV replication and resist viral infection.

Monoclonal antibodies specific for prothrombin fragment 1.2 and their use in a quantitative enzyme-linked immunosorbent assay

1993 ◽  
Vol 39 (4) ◽  
pp. 583-591 ◽  
Author(s):  
M J Hursting ◽  
B T Butman ◽  
J P Steiner ◽  
B M Moore ◽  
M C Plank ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Human Plasma ◽  
Sandwich Elisa ◽  
Peptide Sequence ◽  
Carboxyl Terminus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Thrombotic Risk ◽  
Prothrombin Fragment ◽  
Amino Terminal

Abstract Prothrombin fragment 1.2 (F1.2) is an activation peptide generated during a critical event of blood coagulation, the conversion of prothrombin to thrombin. As a marker of thrombin generation, F1.2 has clinical potential in assessing thrombotic risk and monitoring anticoagulant therapy. In developing a highly specific, monoclonal antibody-based immunoassay of human plasma F1.2, we generated six murine anti-F1.2 monoclonal antibodies, using as immunogen a synthetic peptide (sequence: CGSD-RAIEGR) similar to the unique carboxyl terminus of F1.2. Each antibody bound F1.2 but not prothrombin. Epitope mapping studies with one antibody (5-3B) showed that optimum binding required six to eight amino acids plus a terminal arginine to emulate the F1.2 carboxyl terminus. A quantitative sandwich ELISA for human plasma F1.2 was configured with monoclonal antibody 5-3B as the capture antibody and peroxidase-labeled polyclonal antibodies to the F1.2 amino-terminal region as detector antibodies. Calibrators were prepared by adding purified F1.2, 0-10 nmol/L, to F1.2-depleted plasma. Assay characteristics included the following: mean (+/- SD) analytical recovery of 98% +/- 13%; no interference from lipemia, hemolysis, icterus, or thrombolytic agents; 0.08 nmol/L sensitivity; and mean intra- and interassay imprecision (three lots) < 12% at both low and high concentrations of F1.2.

An indirect sandwich ELISA for the detection of avian influenza H5 subtype viruses using anti-hemagglutinin protein monoclonal antibody

2009 ◽  
Vol 137 (1-2) ◽  
pp. 24-30 ◽  
Author(s):  
Qingping Luo ◽  
Hongliang Huang ◽  
Wei Zou ◽  
Hanbing Dan ◽  
Xuebo Guo ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Avian Influenza ◽  
Sandwich Elisa ◽  
Hemagglutinin Protein ◽  
H5 Subtype
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