scholarly journals Bioreducible Cationic Polymers for Gene Transfection

Biomedicine ◽  
10.5772/38846 ◽  
2012 ◽  
Author(s):  
Chao Lin ◽  
Bo Lou
Keyword(s):  
Gene Transfection ◽  
Cationic Polymers

scholarly journals Biopolymeric Materials Used as Nonviral Vectors: A Review

2021 ◽  
Vol 2 (1) ◽  
pp. 100-109
Author(s):  
Jailson de Araújo Santos ◽  
Daniel Barbosa Liarte ◽  
Alessandra Braga Ribeiro ◽  
Marcia dos Santos Rizzo ◽  
Marcília Pinheiro da Costa ◽  
...  
Keyword(s):  
Chemical Structure ◽  
Web Of Science ◽  
Gene Transfection ◽  
Genetic Material ◽  
Nonviral Vectors ◽  
Cationic Polymers ◽  
Genetic Trait ◽  
Low Diversity ◽  
Different Types ◽  
Materials Used

Bacterial transformation and gene transfection can be understood as being the results of introducing specific genetic material into cells, resulting in gene expression, and adding a new genetic trait to the host cell. Many studies have been carried out to investigate different types of lipids and cationic polymers as promising nonviral vectors for DNA transfer. The present study aimed to carry out a systematic review on the use of biopolymeric materials as nonviral vectors. The methodology was carried out based on searches of scientific articles and applications for patents published or deposited from 2006 to 2020 in different databases for patents (EPO, USPTO, and INPI) and articles (Scopus, Web of Science, and Scielo). The results showed that there are some deposits of patents regarding the use of chitosan as a gene carrier. The 16 analyzed articles allowed us to infer that the use of biopolymers as nonviral vectors is limited due to the low diversity of biopolymers used for these purposes. It was also observed that the use of different materials as nonviral vectors is based on chemical structure modifications of the material, mainly by the addition of cationic groups. Thus, the use of biopolymers as nonviral vectors is still limited to only a few polysaccharide types, emphasizing the need for further studies involving the use of different biopolymers in processes of gene transfer.

scholarly journals Library of Cationic Polymers Composed of Polyamines and Arginine as Gene Transfection Agents

ACS Omega ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 2090-2101 ◽  
Author(s):  
Nino Zavradashvili ◽  
Can Sarisozen ◽  
Giorgi Titvinidze ◽  
Giuli Otinashvili ◽  
Tengiz Kantaria ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymers

Micelle-like luminescent nanoparticles as a visible gene delivery system with reduced toxicity

2015 ◽  
Vol 3 (42) ◽  
pp. 8394-8400 ◽  
Author(s):  
Keni Yang ◽  
Shengliang Li ◽  
Shubin Jin ◽  
Xiangdong Xue ◽  
Tingbin Zhang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Gene Delivery System ◽  
Cationic Polymers ◽  
Luminescent Nanoparticles ◽  
Reduced Toxicity

Luminescent nanoparticles (TPEI) were synthesized to tackle the undesired cytotoxicity of cationic polymers and were also used for visible gene transfection.

Cationic cell penetrating peptide modified SNARE protein VAMP8 as free chains for gene delivery

2018 ◽  
Vol 6 (10) ◽  
pp. 2647-2655 ◽  
Author(s):  
Baizhu Chen ◽  
Chi Wu
Keyword(s):  
Escherichia Coli ◽  
Membrane Protein ◽  
Fusion Proteins ◽  
Gene Transfection ◽  
Cell Penetrating Peptides ◽  
Cell Penetrating Peptide ◽  
Snare Protein ◽  
Cationic Polymers ◽  
N Terminus ◽  
Cell Penetrating

To mimic the effect of cationic polymers, we selected to use vesicle associated membrane protein-8 (VAMP8) and modified its N-terminus with different cationic cell penetrating peptides (CPPs). The modified fusion proteins are expressed in an Escherichia coli system and purified after extraction. These modified VAMP8 proteins are used as free chains for gene transfection, while using bPEI-25k to condense the pDNA.

Efficient Gene Transfection Using Novel Cationic Polymers Poly(hydroxyalkylene imines)

2010 ◽  
Vol 21 (9) ◽  
pp. 1602-1611 ◽  
Author(s):  
Lolita Zaliauskiene ◽  
Ula Bernadisiute ◽  
Ausvydas Vareikis ◽  
Ricardas Makuska ◽  
Ieva Volungeviciene ◽  
...  
Keyword(s):  
Gene Transfection ◽  
Cationic Polymers ◽  
Efficient Gene

scholarly journals In Vitro Evaluation of Lipopolyplexes for Gene Transfection: Comparing 2D, 3D and Microdroplet-Enabled Cell Culture

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3277
Author(s):  
Juan L. Paris ◽  
Filipe Coelho ◽  
Alexandra Teixeira ◽  
Lorena Diéguez ◽  
Bruno F. B. Silva ◽  
...  
Keyword(s):  
Cell Culture ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
3D Culture ◽  
In Vitro Models ◽  
Great Promise ◽  
Cationic Polymers ◽  
Significant Gene ◽  
Biological Performance

Complexes combining nucleic acids with lipids and polymers (lipopolyplexes) show great promise for gene therapy since they enable compositional, physical and functional versatility to be optimized for therapeutic efficiency. When developing lipopolyplexes for gene delivery, one of the first evaluations performed is an in vitro transfection efficiency experiment. Many different in vitro models can be used, and the effect of the model on the experiment outcome has not been thoroughly studied. The objective of this work was to compare the insights obtained from three different in vitro models, as well as the potential limitations associated with each of them. We have prepared a series of lipopolyplex formulations with three different cationic polymers (poly-l-lysine, bioreducible poly-l-lysine and polyethyleneimine), and assessed their in vitro biological performance in 2D monolayer cell culture, 3D spheroid culture and microdroplet-based single-cell culture. Lipopolyplexes from different polymers presented varying degrees of transfection efficiency in all models. The best-performing formulation in 2D culture was the polyethyleneimine lipopolyplex, while lipoplexes prepared with bioreducible poly-l-lysine were the only ones achieving any transfection in microdroplet-enabled cell culture. None of the prepared formulations achieved significant gene transfection in 3D culture. All of the prepared formulations were well tolerated by cells in 2D culture, while at least one formulation (poly-l-lysine polyplex) delayed 3D spheroid growth. These results highlight the need for selecting the appropriate in vitro model depending on the intended application.

The role of PEG architecture and molecular weight in the gene transfection performance of PEGylated poly(dimethylaminoethyl methacrylate) based cationic polymers

Biomaterials ◽  
2011 ◽  
Vol 32 (9) ◽  
pp. 2369-2378 ◽  
Author(s):  
Shrinivas Venkataraman ◽  
Wei Lin Ong ◽  
Zhan Yuin Ong ◽  
Say Chye Joachim Loo ◽  
Pui Lai Rachel Ee ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Transfection ◽  
Cationic Polymers ◽  
Dimethylaminoethyl Methacrylate

Diglycidyl Esters Cross-Linked with Low Molecular Weight Polyethyleneimine for Magnetofection

2015 ◽  
Vol 68 (10) ◽  
pp. 1535 ◽  
Author(s):  
Hao Yu ◽  
Shufeng Li ◽  
Liandong Feng ◽  
Yucheng Liu ◽  
Xiaoliang Qi ◽  
...  
Keyword(s):  
Electrostatic Interactions ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Repeat Unit ◽  
A549 Cells ◽  
Hydroxyl Groups ◽  
Cationic Polymers ◽  
Enhance Gene Expression ◽  
Low Cytotoxicity

Magnetic polyethyleneimine (PEI) complexes have demonstrated to be simple and efficient vectors for enhancing gene transfection. However, the high cytotoxicity of PEI restricts its further application in vivo. In this study, we synthesized several low cytotoxicity biodegradable cationic polymers derived from PEI (Mw 600) linked with diglycidyl tartrate (DT-PEI) or its analogues (diglycidyl succinate (DS-PEI) and diglycidyl malate (DM-PEI); D-PEIs for all 3 polymers). Moreover, a type of biocompatible magnetic nanoparticles (MNPs) with negative charges was prepared to assemble with D-PEIs/DNA complexes via electrostatic interactions. The magnetic ternary complexes have appropriate sizes of 120–150 nm and zeta potential values of ~20–25 mV. The transfection ability and cell viability of D-PEIs increased as the amount of hydroxyl groups increased in the repeat unit, which indicated that increasing the hydroxyl number in the backbone of D-PEIs can enhance gene expression and decrease cytotoxicity in A549 cells. Magnetofection of DT-PEI showed similar transfection efficiency with 30 min incubation; in contrast, the standard incubation time was 4 h. All three magnetic complexes displayed lower cytotoxicity when compared with those of PEI complexes in COS-7 and A549. These results indicated that these series of magnetic PEI derivatives complexes could be potential nanocarriers for gene delivery.

Cyclic Poly(ß-amino ester)s with Enhanced Gene Transfection Activity Synthesized through Intra-molecular Cyclization

2022 ◽  
Author(s):  
Chenfei Wang ◽  
Xiaobei Huang ◽  
Litao Sun ◽  
Qiuxia Li ◽  
Zhili Li ◽  
...  
Keyword(s):  
Free Radical ◽  
Gene Delivery ◽  
Michael Addition ◽  
Topological Structure ◽  
Gene Transfection ◽  
Critical Role ◽  
Ring Closure ◽  
Amino Ester ◽  
Cationic Polymers

Topological structure plays a critical role in gene delivery of cationic polymers. Cyclic poly(ß-amino ester)s (CPAEs) are successfully synthesized via sequential Michael addition and free radical initiating ring-closure reaction. CPAE...

On the Mechanism by which Cell Contact Induces the Release Reaction of Blood Platelets; the Effect of Cationic Polymers

1972 ◽  
Vol 27 (01) ◽  
pp. 121-133 ◽  
Author(s):  
P Massini ◽  
E. F Lüscher
Keyword(s):  
Human Blood ◽  
Calcium Ions ◽  
Blood Platelets ◽  
Cell Contact ◽  
Second Phase ◽  
Cationic Polymers ◽  
Release Reaction ◽  
Per Se ◽  
Human Blood Platelets

SummaryHuman blood platelets are aggregated by the basic polymers polylysine and DEAE- dextran. Under certain conditions a second phase of aggregation, concomitant with the release reaction, is elicited. The presence of ADP, calcium ions and a plasmatic cofactor within the primary aggregates are necessary for the induction of the release reaction. These experiments demonstrate that cell contact per se does not lead to a release reaction ; in order to become effective it must take place in the presence of ADP.

Sign in / Sign up

Export Citation Format

Share Document