scholarly journals BRAF V600E Mutation Detection Using High Resolution Probe Melting Analysis

10.5772/37111 ◽  
2012 ◽  
Author(s):  
Jennifer E. ◽  
Ann Chua ◽  
Joseph W. ◽  
Aravind Shivasami ◽  
Irene Kanter ◽  
...  
Keyword(s):  
High Resolution ◽  
Mutation Detection ◽  
Braf V600e Mutation ◽  
Braf V600e ◽  
Melting Analysis

scholarly journals High-Resolution Melting Is a Sensitive, Cost-Effective, Time-Saving Technique for BRAF V600E Detection in Thyroid FNAB Washing Liquid: A Prospective Cohort Study

2015 ◽  
Vol 4 (2) ◽  
pp. 73-81 ◽  
Author(s):  
Marco Marino ◽  
Maria Laura Monzani ◽  
Giulia Brigante ◽  
Katia Cioni ◽  
Bruno Madeo ◽  
...  
Keyword(s):  
Cohort Study ◽  
High Resolution ◽  
Molecular Analysis ◽  
Prospective Cohort Study ◽  
Prospective Cohort ◽  
Large Scale ◽  
High Resolution Melting ◽  
Cost Effective ◽  
Braf V600e Mutation ◽  
Braf V600e

Objective: The diagnostic accuracy of thyroid fine needle aspiration biopsy (FNAB) can be improved by the combination of cytological and molecular analysis. In this study, washing liquids of FNAB (wFNAB) were tested for the BRAF V600E mutation, using the sensitive and cost-effective technique called high-resolution melting (HRM). The aim was to demonstrate the feasibility of BRAF analysis in wFNAB and its diagnostic utility, combined with cytology. Design: Prospective cohort study. Methods: 481 patients, corresponding to 648 FNAB samples, were subjected to both cytological (on cells smeared onto a glass slide) and molecular analysis (on fluids obtained washing the FNAB needle with 1 ml of saline) of the same aspiration. BRAF V600E analysis was performed by HRM after methodological validation for application to wFNAB (technique sensitivity: 5.4%). Results: The cytological results of the FNAB were: 136 (21%) nondiagnostic (THY1); 415 (64%) benign (THY2); 80 (12.4%) indeterminate (THY3); 9 (1.4%) suspicious for malignancy (THY4); 8 (1.2%) diagnostic of malignancy (THY5). The BRAF V600E mutation was found in 5 THY2, 2 THY3, 6 THY4 and 6 THY5 samples. Papillary carcinoma diagnosis was histologically confirmed in all BRAF+ thyroidectomized patients. BRAF combined with cytology improved the diagnostic value compared to cytology alone in a subgroup of 74 operated patients. Conclusions: HRM was demonstrated to be a feasible method for BRAF analysis in wFNAB. Thanks to its sensitivity and cost-effectiveness, it might be routinely used on a large scale in clinical practice. In perspective, standby wFNAB samples could be analyzed a posteriori in case of indeterminate cytology and/or suspicious findings on ultrasound.

Tumor heterogeneity: Evidence from BRAF V600E mutation detection

2008 ◽  
Vol 26 (15_suppl) ◽  
pp. 20022-20022 ◽  
Author(s):  
A. J. Litterman ◽  
D. Pollens ◽  
M. A. Warycha ◽  
R. L. Shapiro ◽  
R. S. Berman ◽  
...  
Keyword(s):  
Tumor Heterogeneity ◽  
Mutation Detection ◽  
Braf V600e Mutation ◽  
Braf V600e

Comparison of Immunohistochemistry and DNA Sequencing for BRAF V600E Mutation Detection in Mandibular Ameloblastomas

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Barbara M.R. da Silva Marcelino ◽  
Guilherme K. Parise ◽  
Alan M. do Canto ◽  
Laurindo M. Sassi ◽  
Dmitry J.S. Sarmento ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Mutation Detection ◽  
Braf V600e Mutation ◽  
Braf V600e

Evaluation of BRAF V600E mutation detection assays for purpose of clinical tumor sample analysis

2010 ◽  
Author(s):  
Russell M. Walker ◽  
David I. Lichter ◽  
William L. Trepicchio ◽  
Elena Izmailova
Keyword(s):  
Mutation Detection ◽  
Braf V600e Mutation ◽  
Braf V600e ◽  
Sample Analysis ◽  
Tumor Sample
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