scholarly journals KRAS mutation detection by high-resolution melting analysis significantly predicts clinical benefit of cetuximab in metastatic colorectal cancer

2012 ◽  
Vol 107 (4) ◽  
pp. 626-631 ◽  
Author(s):  
U Malapelle ◽  
C Carlomagno ◽  
M Salatiello ◽  
A De Stefano ◽  
C De Luca ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Resolution ◽  
Metastatic Colorectal Cancer ◽  
Clinical Benefit ◽  
Kras Mutation ◽  
Mutation Detection ◽  
High Resolution Melting ◽  
High Resolution Melting Analysis ◽  
Melting Analysis

scholarly journals A rational two-step approach to KRAS mutation testing in colorectal cancer using high resolution melting analysis and pyrosequencing

BMC Cancer ◽  
2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Elisabeth Mack ◽  
Kathleen Stabla ◽  
Jorge Riera-Knorrenschild ◽  
Roland Moll ◽  
Andreas Neubauer ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Resolution ◽  
Kras Mutation ◽  
High Resolution Melting ◽  
Mutation Testing ◽  
High Resolution Melting Analysis ◽  
Melting Analysis

Increased sensitivity of KRAS mutation detection by high-resolution melting analysis of COLD-PCR products

2010 ◽  
Vol 31 (12) ◽  
pp. 1366-1373 ◽  
Author(s):  
Lasse S. Kristensen ◽  
Iben L. Daugaard ◽  
Mariann Christensen ◽  
Stephen Hamilton-Dutoit ◽  
Henrik Hager ◽  
...  
Keyword(s):  
High Resolution ◽  
Kras Mutation ◽  
Mutation Detection ◽  
High Resolution Melting ◽  
High Resolution Melting Analysis ◽  
Pcr Products ◽  
Melting Analysis ◽  
Increased Sensitivity

scholarly journals SLCO1B3 screening in colorectal cancer patients using High-Resolution Melting Analysis method and immunohistochemistry

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769117 ◽  
Author(s):  
Lampri Evangeli ◽  
Sainis Ioannis ◽  
Kounnis Valentinos ◽  
Mitselou Antigony ◽  
Ioachim Elli ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
High Resolution ◽  
Cancer Patients ◽  
High Resolution Melting ◽  
Organic Anion ◽  
High Resolution Melting Analysis ◽  
Organic Anion Transporting Polypeptide ◽  
Melting Analysis ◽  
Colorectal Cancer Patients ◽  
Gene Alterations

Personalized medicine has made some major advances in colorectal cancer, but new biomarkers still remain a hot issue as an emerging tool with potential prognostic and therapeutic potential. We investigated for SLCO1B3 gene alterations and protein expression in colorectal cancer, using the novel high-resolution melting analysis technique and immunohistochemistry. Formalin-fixed paraffin-embedded tumor samples from 30 colorectal cancer patients were used. The screening for gene alterations was done by high-resolution melting analysis for all exons of SLCO1B3 gene. Organic anion-transporting polypeptide 1B3 protein expression was assessed by immunohistochemistry using the monoclonal mouse MDQ antibody. High level of polymorphism was observed in the SLCO1B3 gene. We identified three previously reported polymorphisms in exons 7, 12, and 14, 699G>A, 1557A>G, and 1833G>A, respectively. In the exon 5, one variant seems to correspond to an as yet unknown SLCO family member. The immunohistochemical study revealed that organic anion-transporting polypeptide 1B3 was expressed in 27/30 samples. Of great interest, the three samples, which were immunohistochemically negative, all appeared to accommodate mutations which lead to either early stop codons or other conformations of the tertiary protein structures affecting the antibody-epitope binding. The results of this study are of much interest as high-resolution melting analysis proved to be a reliable and rapid genotyping/scanning method for mutation detection of SLCO1B3 gene.

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