scholarly journals Applications of Flow Cytometry in Solid Organ Allogeneic Transplantation

10.5772/36895 ◽  
2012 ◽  
Author(s):  
Dimitrios Kirmizis ◽  
Dimitrios Chatzidimitriou ◽  
Fani Chatzopoulou ◽  
Lemonia Skoura ◽  
Gregory Myserlis
Keyword(s):  
Flow Cytometry ◽  
Allogeneic Transplantation ◽  
Solid Organ

scholarly journals Flow Cytometry and Solid Organ Transplantation: A Perfect Match

2014 ◽  
Vol 43 (8) ◽  
pp. 756-774 ◽  
Author(s):  
Orla Maguire ◽  
Joseph D. Tario ◽  
Thomas C. Shanahan ◽  
Paul K. Wallace ◽  
Hans Minderman
Keyword(s):  
Flow Cytometry ◽  
Organ Transplantation ◽  
Perfect Match ◽  
Solid Organ Transplantation ◽  
Solid Organ

scholarly journals Novel Diagnostic and Therapeutic Approach to Antibody-Mediated Rejections in Heart Transplantation

2020 ◽  
Author(s):  
Takuya Watanabe ◽  
Norihide Fukushima
Keyword(s):  
Flow Cytometry ◽  
Heart Transplantation ◽  
Membrane Attack Complex ◽  
Hla Antigens ◽  
Human Leukocyte ◽  
Classical Pathway ◽  
Cytotoxicity Test ◽  
Solid Organ ◽  
Heart Graft ◽  
Cardiac Graft

Despite the improvement of immunosuppressive therapy in heart transplantation (HTx), antibody-mediated rejection (AMR) is still a great obstacle to prolong cardiac graft survival. Anti-donor-specific antibodies (DSAs), especially anti-donor human leukocyte antigen (HLA) antibody, lead to heart graft failure resulting in hemodynamic consequence and often in the recipient death. To prevent hyperacute rejection, prospective complement-dependent cytotoxicity test has been performed in every cardiac donor in Japan. But in other solid organ transplantations, flow cytometry crossmatch has been recently recommended to crossmatch to select the recipient in Japan as well as the world. However, flow cytometry is too sensitive to select the recipient, because not all DSAs determined by flow cytometry are cytotoxic to the cardiac graft. On the first complement classical pathway, alloantibodies bind to HLA antigens on cells of the graft and then recruit C1q, which is essential to make membrane attack complex and kill the cell. We review a role of the novel monitoring method of complement pathway regarding C1q in occurrence of AMR and its diagnostic and therapeutic significance in managing AMR in HTx.

scholarly journals EBV-Associated Post-Transplant Lymphoproliferative Disease (PTLD) in Allogeneic Transplantation

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Cutini I ◽  
◽  
Peruzzi B ◽  
Caporale R ◽  
Nozzoli C ◽  
...  
Keyword(s):  
Myeloid Leukemia ◽  
Solid Organ Transplantation ◽  
B Cell Lymphoma ◽  
Allogeneic Transplantation ◽  
Lymphoproliferative Disease ◽  
Solid Organ ◽  
Hematopoietic Stem ◽  
Post Transplant ◽  
Control Virus ◽  
Life Threatening

Post-Transplant Lymphoproliferative Disease (PTLD) following both Solid Organ Transplantation (SOT) and Hematopoietic Stem Cell Transplantation (HSCT) is a rare life-threatening complication. The majority of PLTDs are associated to Epstein Bar Virus (EBV) [1] reactivation, usually in the early phase [2] after transplant, when the patient is severely immunocompromised and is unable to control virus replication [3]. Despite the mortality of EBV-associated PTLD has been reduced over the years, the different histological patterns of its presentation, ranging from indolent to high grade B cell lymphoma, still play a role in the outcome. Herein, we report the case of a 60-years-old man diagnosed with acute myeloid leukemia who underwent allogeneic transplantation and developed a fatal Hemophagocytic Histiocytosis (HLH) secondary to an aggressive EBV-associated PTLD, not responding to a rituximab-based treatment.

scholarly journals Clinical Relevance of Direct Quantification of pp65 Antigenemia Using Flow Cytometry in Solid Organ and Stem Cell Transplant Recipients

2000 ◽  
Vol 38 (9) ◽  
pp. 3143-3149 ◽  
Author(s):  
Anne-Sophie Poirier-Toulemonde ◽  
Noel Milpied ◽  
Diego Cantarovich ◽  
Jean-François Morcet ◽  
Sylviane Billaudel ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Stem Cell Transplant ◽  
Laboratory Data ◽  
Solid Organ ◽  
Cell Transplant ◽  
Cmv Infection ◽  
Pp65 Antigenemia ◽  
Cmv Disease ◽  
Direct Quantification

A total of 1,305 blood samples from 85 solid organ transplant (SOT) recipients and 25 stem cell transplant (SCT) recipients at risk for cytomegalovirus (CMV) infection were prospectively collected and tested using the shell vial assay (SVA) and a leukocytic qualitative PCR (q-PCR). Of these, 462 specimens were further tested by direct quantification of CMV antigenemia by flow cytometry (FC-Ag), 125 were tested with a quantitative competitive PCR, and 200 were tested for pp65 antigenemia using the slide method (S-Ag). Laboratory data were statistically analyzed according to the presence of CMV-related symptoms. In SOT and SCT recipients, active CMV infection occurred in 63.5 and 36%, respectively, and CMV disease occurred in 53 and 24%, respectively. FC-Ag results correlated better with q-PCR and S-Ag than with SVA. The first test found to be positive during follow-up was FC-Ag in 73% of cases. In SOT recipients, FC-Ag showed the highest sensitivity and negative predictive value for the diagnosis of any grade of CMV disease. For FC-Ag, the threshold beyond which CMV disease was highly probable seemed to lie at 0.20% positive polymorphonuclear leukocytes. FC-Ag appears to be a useful test for the early detection of CMV infection and the prediction of CMV disease.

Minimal Residual Disease (MRD) Evaluation by Flow Cytometry in Acute Leukemia Patients Undergoing Allogeneic Stem Cell Transplantation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1103-1103
Author(s):  
Maria-Belen Vidriales ◽  
Jose J. Pérez ◽  
Consuelo López-Berges ◽  
Norma C. Gutierrez ◽  
Juana Ciudad ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Minimal Residual Disease ◽  
Allogeneic Stem Cell Transplantation ◽  
Acute Leukaemia ◽  
Prognostic Value ◽  
Residual Disease ◽  
Allogeneic Transplantation ◽  
Minimal Residual

Abstract Investigation of minimal residual disease (MRD) in acute leukemias by immunophenotyping is increasingly used for disease monitoring. Most MRD studies using flow cytometry techniques have focused on patients treated with conventional chemotherapy, while information on its value in patients undergoing allogeneic transplantation is scanty. The aim of the present study is to evaluate whether or not immunophenotypical assessment of MRD could also be a valuable tool in patients undergoing allogeneic transplantation for acute leukaemia. For that purpose we have analysed the level of MRD before and after (month +3) transplantation, by multiparameter flow cytometry, in a series of 38 acute leukaemia patients (26 ALL cases -20 cases in 1st CR and 6 in 2nd CR- and 12 AML cases -all of them in 1st CR), that showed an aberrant immunophenotype at diagnosis. Although the level of MRD in the BM obtained before transplantation showed a tendency to predict RFS, differences did not reached statistical significance. By contrast, the evaluation of the BM obtained 3 months after allo-transplantation had significant prognostic value. Thus, patients with low MRD levels (<0.05% for ALL and <0.2% for AML) (n=17) had a significantly longer RFS than patients with MRD positive (median RFS: 40 vs 16 months) (p=0.001). Multivariate analysis showed that the immunological evaluation of MRD after transplantation had independent prognostic value for RFS in acute leukaemia, together with age, WBC count, and cytogenetics. Our results suggest that immunological analysis of MRD should be included in the follow-up evaluation of acute leukaemia patients undergoing allogeneic stem cell transplantation.

CD4+CD28+ T Lymphoctye Subsets Decreased in Healthy Donors after G-CSF Mobilization.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 3922-3922
Author(s):  
Jianyu Weng ◽  
Xin Du ◽  
Jianjun Zhang ◽  
Suijin Wu ◽  
Zesheng Lu ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Peripheral Blood ◽  
Allogeneic Transplantation ◽  
Peripheral Blood Stem Cells ◽  
Color Flow ◽  
Cd8 Cells ◽  
Healthy Donors ◽  
Blood Stem Cells ◽  
Safety Considerations

Abstract Objective Allogeneic transplantation of recombinant human granulocyte colony-stimulating factor (rhG-CSF) -mobilized peripheral blood stem cells (PBSCs) is now being increasingly performed, but safety considerations for hematologically normal PBSC donors have not been fully addressed. The effection of G-CSF on donors’ lymphocytes have not been defined clearly. Our study was to detect and analyze the phenotypical and functional properties of lymphocytes from allo-PBSC donors treated with recombinant human G-CSF by flow cytometry. Methods Thirty-four HLA-identical sibling donors (13male, 21female; median age, 35 years) were treated by subcutaneous injection with rhG-CSF at a dose of 5 μg/kg twice daily for 4–6 consecutive days to mobilize HSCs to the peripheral blood. Leukapheresis was performed using a continuous flow blood cell separator (COBE Spectra, Lakewood, CO) on 1 to 2 consecutive days beginning on day 4 of rhG-CSF administration. Donor blood samples, which were obtained before the first administration of G-CSF (pre-G), on day 4 of G-CSF administration (post-G), and one week after the last rhG-CSF were analyzed by 3-color flow cytometry. Monoclonal antibodies included: CD3, CD4, CD8, CD20, CD16, CD56, CD25, CD69, CD45RA, CD45RO, CD28, CD95. Results Absolute counts of lymphocytes, B cells, T cells, CD4+ and CD8+cells post-G were significantly elevated two more times than pre-G (P<0.05), but these changes recovered when the last G-CSF administrated one week later. The percentage of CD3+, CD4+, CD8+ and the ratio of CD4 and CD8 T cells had no significantly difference between pre-G and post-G. The percentage of CD4+CD25+, CD4+CD45RO+ and CD4+CD28+ T cells were significantly decreased post-G (45.26±9.68% to 37.34±11.12%; 65.53±13.74% to 52.32±13.47%; 94.75±4.02% to 91.74±8.72%, P=0.00329, 0.0003 and 0.0947, respectively). We found that CD4+CD28+ T cells(91.77 ± 6.15, P=0.00218) were still lower than pre-G when stopped administrate G-CSF one week later, while others changes have recovered to the Pre-G level. Conclusion: The study shows that CD4+CD28+ T lymphoctye subsets were still lower than pre-G, although most of other change recover one week later after mobilization

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