scholarly journals Quantification of minimal residual disease levels by flow cytometry at time of transplant predicts outcome after myeloablative allogeneic transplantation in ALL

2012 ◽  
Vol 48 (3) ◽  
pp. 396-402 ◽  
Author(s):  
J Sanchez-Garcia ◽  
J Serrano ◽  
J Serrano-Lopez ◽  
P Gomez-Garcia ◽  
F Martinez ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Allogeneic Transplantation ◽  
Minimal Residual

Minimal Residual Disease (MRD) Evaluation by Flow Cytometry in Acute Leukemia Patients Undergoing Allogeneic Stem Cell Transplantation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1103-1103
Author(s):  
Maria-Belen Vidriales ◽  
Jose J. Pérez ◽  
Consuelo López-Berges ◽  
Norma C. Gutierrez ◽  
Juana Ciudad ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Stem Cell Transplantation ◽  
Minimal Residual Disease ◽  
Allogeneic Stem Cell Transplantation ◽  
Acute Leukaemia ◽  
Prognostic Value ◽  
Residual Disease ◽  
Allogeneic Transplantation ◽  
Minimal Residual

Abstract Investigation of minimal residual disease (MRD) in acute leukemias by immunophenotyping is increasingly used for disease monitoring. Most MRD studies using flow cytometry techniques have focused on patients treated with conventional chemotherapy, while information on its value in patients undergoing allogeneic transplantation is scanty. The aim of the present study is to evaluate whether or not immunophenotypical assessment of MRD could also be a valuable tool in patients undergoing allogeneic transplantation for acute leukaemia. For that purpose we have analysed the level of MRD before and after (month +3) transplantation, by multiparameter flow cytometry, in a series of 38 acute leukaemia patients (26 ALL cases -20 cases in 1st CR and 6 in 2nd CR- and 12 AML cases -all of them in 1st CR), that showed an aberrant immunophenotype at diagnosis. Although the level of MRD in the BM obtained before transplantation showed a tendency to predict RFS, differences did not reached statistical significance. By contrast, the evaluation of the BM obtained 3 months after allo-transplantation had significant prognostic value. Thus, patients with low MRD levels (<0.05% for ALL and <0.2% for AML) (n=17) had a significantly longer RFS than patients with MRD positive (median RFS: 40 vs 16 months) (p=0.001). Multivariate analysis showed that the immunological evaluation of MRD after transplantation had independent prognostic value for RFS in acute leukaemia, together with age, WBC count, and cytogenetics. Our results suggest that immunological analysis of MRD should be included in the follow-up evaluation of acute leukaemia patients undergoing allogeneic stem cell transplantation.

Early assessment of minimal residual disease in AML by flow cytometry during aplasia identifies patients at increased risk of relapse

Leukemia ◽  
2014 ◽  
Vol 29 (2) ◽  
pp. 377-386 ◽  
Author(s):  
T Köhnke ◽  
D Sauter ◽  
K Ringel ◽  
E Hoster ◽  
R P Laubender ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Early Assessment ◽  
Increased Risk ◽  
Minimal Residual ◽  
Risk Of Relapse

Flow Cytometry for Minimal Residual Disease

2015 ◽  
Vol 15 ◽  
pp. e5
Author(s):  
B. Paiva ◽  
M.B. Vidriales ◽  
N. Puig ◽  
M.A. Montaban ◽  
L. Cordón ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Minimal Residual Disease ◽  
Residual Disease ◽  
Minimal Residual
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