scholarly journals A Modified Enzyme Immunoassay Method for Determination of cAMP in Plant Cells

10.5772/35385 ◽  
2012 ◽  
Author(s):  
Lidia A. ◽  
Anatoly S. ◽  
Nadya V. ◽  
Olga V.
Keyword(s):  
Enzyme Immunoassay ◽  
Plant Cells ◽  
Modified Enzyme

Determination of cAMP in plant cells by a modified enzyme immunoassay method

2010 ◽  
Vol 30 (1) ◽  
pp. 125-132 ◽  
Author(s):  
L. A. Lomovatskaya ◽  
A. S. Romanenko ◽  
N. V. Filinova ◽  
L. V. Dudareva
Keyword(s):  
Enzyme Immunoassay ◽  
Plant Cells ◽  
Modified Enzyme

A Simplified Method of Determination of cAMP in Plants Using Modified Enzyme Immunoassay

2005 ◽  
Vol 32 (6) ◽  
pp. 567-570 ◽  
Author(s):  
L. A. Lomovatskaya ◽  
A. S. Romanenko ◽  
N. V. Krivolapova
Keyword(s):  
Enzyme Immunoassay ◽  
Method Of Determination ◽  
Simplified Method ◽  
Modified Enzyme

scholarly journals AB0110 DETERMINING THE AMOUNT OF COPPER–CONTAINING PROTEIN, ITS BIOCHEMICAL AND IMMUNOLOGIC ACTIVITY IN RHEUMATOID ARTHRITIS PATIENTS

2021 ◽  
Vol 80 (Suppl 1) ◽  
pp. 1083.2-1084
Author(s):  
O. Rusanova ◽  
A. Trofimenko ◽  
N. Emelyanov ◽  
O. Emelyanova
Keyword(s):  
Rheumatoid Arthritis ◽  
Disease Activity ◽  
Enzyme Immunoassay ◽  
Oxidase Activity ◽  
Mean Value ◽  
Hospital Treatment ◽  
Diagnostic Agent ◽  
Immunologic Activity ◽  
Rheumatoid Arthritis Activity

Background:Production of antibodies to ceruloplasmin (CP) in rheumatoid arthritis is an issue that has not been studied well enough. It was not by chance that this copper–containing alpha 2-glycorpoteid of blood plasma showing multienzymatic properties was chosen as an object of investigation. Data on the content and activity of CP in the blood of rheumatoid arthritis patients are contradictory, which has to do with different approaches to selection of patients and different measuring methods.Objectives:Improving diagnosis of rheumatoid arthritis by determination of antibodies to CP as well as its amount and enzymatic activity.Methods:We studied the serum from 30 apparently healthy individuals, and 108 rheumatoid arthritis patients. Antibodies to CP were determined by enzyme immunoassay using immobilized granulated antigen preparations (modification by Gontar et al, 2002). The amount of CP was determined by enzyme immunoassay according to the method of I.S. Kuzmina et al (1991) using commercial diagnostic agent manufactured by Mechnikov Research Institute for Vaccines and Sera.Results:Enzyme immunoassay showed a mean level of CP antibodies in donor sera of 0,020±0,006 optical density units. The level of normal values of specific antibodies determined as M±2σ included an extinction value in the range 0 – 0,086. The mean value of oxidase activity and the amount of CP in healthy people was 716±26,3 and 921±32 ng/ml, correspondingly. In the process of study we revealed a reliable increase in CP antibody count, the activity and amount of CP in patients with rheumatoid arthritis while in all cases the parameters under study correlated with the degree of disease activity (p<0,05): at activity degree I CP antibodies were 0,098±0,011; CP activity was 954±48,1; CP amount was 1292±73,4. At activity degree II CP antibodies were 0,138±0,007; CP activity was 1163±39,6; CP amount was 1763±69,3. At activity degree III, CP antibodies were 0,182±0,015; CP activity was 1368±89,5; CP amount was 1794±102,8. After a course of hospital treatment was completed, we noted a reliable decrease in the activity and amount of CP (at degree I of rheumatoid arthritis activity p<0,001, at degree II of rheumatoid arthritis activity p<0,01for both parameters; at degree III, p<0,05) compared with baseline findings. A decrease in CP antibodies shows decelerated dynamics, especially in patients with pronounced disease activity, which indicates severe disorders in the immunity that cannot be cured completely within 30 – 40 days of hospital treatment course.Conclusion:Determination of CP antibodies, as well as quantitative content of CP and its oxidase activity can serve as indicators of the activity of rheumatoid arthritis, as well as an accessory criterion of the effectiveness of administered therapy.Disclosure of Interests:None declared

Inhibition of carbon fixation as a function of zinc uptake in natural phytoplankton assemblages

1979 ◽  
Vol 59 (4) ◽  
pp. 937-949 ◽  
Author(s):  
Anthony G. Davies ◽  
Jillian A. Sleep
Keyword(s):  
Carbon Fixation ◽  
Natural Populations ◽  
Plant Cells ◽  
Toxic Metal ◽  
Vitamin B ◽  
Phytoplankton Assemblages ◽  
Metal Contents ◽  
Rate Limiting ◽  
Natural Phytoplankton

There is now a substantial body of evidence that the growth rates of phytoplankton in culture are more closely related to the cellular levels of the rate-limiting constituent, be it a nutrient, micronutrient or toxic metal, than to the concentrations in the supporting medium; nitrate, Caperon (1968); phosphate, Fuhs (1969); silicate, Paasche (1973); vitamin B12, Droop (1968); iron, Davies (1970); mercury, Davies (1974); cadmium, Davies (1978a). This has suggested the requirement for a technique which would allow the determination of comparable relationships for natural populations of phytoplankton - how, for instance, their carbon fixation rates depend upon the metal contents of the plant cells. Although the effects of metals upon carbon fixation in phytoplankton assemblages from several different sea areas have already been examined (Knauer & Martin, 1972; Patin et al. 1974; Zingmark & Miller, 1975; Ibragim & Patin, 1976) no data seem to have been obtained on the levels of the metals present in the phytoplankton at the time of the measurements.

Measurement of progesterone in sheep using a commercial ELISA kit for human plasma

2021 ◽  
pp. 104063872110435
Author(s):  
Valeria Pasciu ◽  
Maria Nieddu ◽  
Elena Baralla ◽  
Cristian Porcu ◽  
Francesca Sotgiu ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Human Plasma ◽  
Method Validation ◽  
Plasma Progesterone ◽  
Freeze Thaw ◽  
Elisa Kit ◽  
Stability Data ◽  
Human Use ◽  
Species Specific

Determination of serum or plasma progesterone (P4) concentrations is important to recognize pregnant and non-pregnant ewes, and also to predict the number of carried lambs. The 2 most common methodologies for the detection of plasma P4 are radioimmunoassay (RIA) and enzyme immunoassay (EIA). RIA is very expensive, and not all laboratories are equipped to perform this test; EIA is commercially available for human use, but only a few companies produce species-specific kits, which are expensive. We verified for ovine plasma a less expensive and easily available ELISA kit (DiaMetra) designed to quantify P4 in humans. Pools of ovine and human plasma were used to compare repeatability, accuracy, sensitivity, and stability of P4 measured by the DiaMetra kit. Repeatability data were within 15%, and accuracy values were ~90% for both plasma matrices. Stability data showed a loss of <20% for freeze–thaw and <30% for 30-d storage. All parameters were acceptable under international guidelines for method validation. The human ELISA kit was used successfully to quantify plasma P4 in 26 ewes during pregnancy until delivery. P4 concentrations were also correlated with the number of carried lambs.

Reverse Enzyme Immunoassay for the Determination of Lolium perenne IgE Antibodies

1983 ◽  
Vol 72 (2) ◽  
pp. 184-187 ◽  
Author(s):  
M. Cuevas ◽  
I. Moneo ◽  
V. Ureña ◽  
J. Dominguez ◽  
A. Bootello
Keyword(s):  
Enzyme Immunoassay ◽  
Lolium Perenne ◽  
Ige Antibodies
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