scholarly journals Biochemical Analysis of Halophilic Dehydrogenases Altered by Site-Directed Mutagenesis

10.5772/34565 ◽  
2013 ◽  
Author(s):  
J. Esclapez ◽  
M. Camacho ◽  
C. Pire ◽  
M.J. Bonete
Keyword(s):  
Biochemical Analysis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

scholarly journals Identification of the catalytic residues of the first family of β(1–3)glucanosyltransferases identified in fungi

2000 ◽  
Vol 347 (3) ◽  
pp. 741-747 ◽  
Author(s):  
Isabelle MOUYNA ◽  
Michel MONOD ◽  
Thierry FONTAINE ◽  
Bernard HENRISSAT ◽  
Barbara LÉCHENNE ◽  
...  
Keyword(s):  
Cluster Analysis ◽  
Active Site ◽  
Biochemical Analysis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Sequence Comparisons ◽  
Catalytic Residues ◽  
Hydrophobic Cluster Analysis ◽  
Hydrophobic Cluster ◽  
New Family

A new family of glycosylphosphatidylinositol-anchored β(1-3)glucanosyltransferases (Gelp), recently identified and characterized in the filamentous fungus Aspergillus fumigatus, showed functional similarity to the Gas/Phr/Epd protein families, which are involved in yeast morphogenesis. Sequence comparisons and hydrophobic cluster analysis (HCA) showed that all the Gas/Phr/Epd/Gel proteins belong to a new family of glycosylhydrolases, family 72. We confirmed by site-directed mutagenesis and biochemical analysis that the two conserved glutamate residues (the putative catalytic residues of this family, as determined by HCA) are involved in the active site of this family of glycosylhydrolases.

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Site-directed mutagenesis on alkaline phosphatasefrompseudomonas aeruginosapao1 for enhanced activity

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
UMA SELVARAJ ◽  
THIRUMALAI MUTHUKUMARESAN ◽  
GAYATHRI VIJAYENDRAN ◽  
SENTHIL KUMAR DEVAN ◽  
VENU BABU P ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enhanced Activity

scholarly journals Activation of anthrachamycin biosynthesis in Streptomyces chattanoogensis L10 by site-directed mutagenesis of rpoB

2019 ◽  
Vol 20 (12) ◽  
pp. 983-994
Author(s):  
Zi-yue Li ◽  
Qing-ting Bu ◽  
Jue Wang ◽  
Yu Liu ◽  
Xin-ai Chen ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Streptomyces Chattanoogensis
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