scholarly journals Immunohistochemical Correlation of Novel Biomarkers with Neurodegeneration in Rat Models of Brain Injury

10.5772/34184 ◽  
2012 ◽  
Author(s):  
Shyam Gajavelli ◽  
Amade Bregy ◽  
Markus Spurlock ◽  
Daniel Diaz ◽  
Stephen Burks ◽  
...  
Keyword(s):  
Brain Injury ◽  
Rat Models ◽  
Novel Biomarkers

COMPARISON OF EXPERIMENTAL RAT MODELS OF EARLY BRAIN INJURY AFTER SUBARACHNOID HEMORRHAGE

Neurosurgery ◽  
2009 ◽  
Vol 65 (2) ◽  
pp. 331-343 ◽  
Author(s):  
Jin-Yul Lee ◽  
Oren Sagher ◽  
Richard Keep ◽  
Ya Hua ◽  
Guohua Xi
Keyword(s):  
Subarachnoid Hemorrhage ◽  
Brain Injury ◽  
Early Brain Injury ◽  
Rat Models

scholarly journals Plasma extracellular vesicles as a source of biomarkers in traumatic brain injury

2020 ◽  
pp. 1-8
Author(s):  
Ross C. Puffer ◽  
Luz M. Cumba Garcia ◽  
Benjamin T. Himes ◽  
Mi-Yeon Jung ◽  
Frederic B. Meyer ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Extracellular Vesicles ◽  
Noncoding Rna ◽  
Altered Consciousness ◽  
Biologically Relevant ◽  
Potential Biomarker ◽  
Diagnosis And Classification ◽  
Novel Biomarkers ◽  
Organismal Development

OBJECTIVEThe objective of this study was to isolate extracellular vesicles (EVs) from plasma in a cohort of patients with traumatic brain injury (TBI) and analyze their contents for novel biomarkers that could prove useful for rapid diagnosis and classification of brain injury during initial evaluation.METHODSPlasma EVs were isolated by serial ultracentrifugation from patients with TBI (n = 15) and healthy controls (n = 5). Samples were obtained from the TRACK-TBI biorepository (2010–present). Size and concentration were determined by nanoparticle tracking. Glial fibrillary acidic protein (GFAP) concentration was determined in EV protein. EV RNA was isolated and deep sequencing of short noncoding RNA was performed.RESULTSPlasma EVs are physically similar but contained approximately 10 times more GFAP in TBI patients with altered consciousness than patients and controls with normal consciousness. Eleven highly differentially expressed microRNAs (miRNAs) were identified between these groups. Genes targeted by these miRNAs are highly associated with biologically relevant cellular pathways, including organismal injury, cellular development, and organismal development. Multiple additional coding and noncoding RNA species with potential biomarker utility were identified.CONCLUSIONSIsolating plasma EVs in patients with TBI is feasible. Increased GFAP concentration—a validated plasma TBI marker—in EVs from TBI patients with altered consciousness, along with differential expression of multiple miRNAs targeting TBI-relevant pathways, suggests that EVs may be a useful source of TBI biomarkers. Additional evaluation in larger patient cohorts is indicated.

scholarly journals Effects of TLR3 and TLR9 Signaling Pathway on Brain Protection in Rats Undergoing Sevoflurane Pretreatment during Cardiopulmonary Bypass

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Zhou Nan ◽  
Zhou Jin ◽  
Cao Huijuan ◽  
Zhang Tiezheng ◽  
Chen Keyan
Keyword(s):  
Brain Injury ◽  
Signaling Pathway ◽  
Neuronal Apoptosis ◽  
Sham Group ◽  
Brain Protection ◽  
Rat Models ◽  
Tlr9 Expression ◽  
Sd Rats ◽  
Tlr3 Expression ◽  
Tlr9 Signaling

Objective. To investigate the effects of TLR3 and TLR9 signaling pathway on brain injury during CPB in rats pretreated with sevoflurane and its possible molecular mechanism. Methods. SD rats were randomly assigned to sham group, CPB group, and Sev group. Brain tissue was obtained at before CPB (T0), at CPB for 30 minutes (T1), 1 hour after CPB (T3), and 3 hours after CPB (T5). ELISA was used to measure S100-β and IL-6. Western blot was utilized to determine TLR3 and TLR9 expression. TUNEL was applied to detect neuronal apoptosis. Results. Compared with CPB group, at T1, at termination after 1 hour of CPB (T2), T3, 2 hours after CPB (T4) and T5, S100-β and IL-6 decreased in Sev group. Compared with CPB group, IFN-β were increased in Sev group, except T0. Compared with CPB group, TLR3 expression increased, and TLR9 and NF-κB decreased in Sev group. The apoptotic neurons were less in Sev group than in CPB group (P<0.05). Conclusion. Sevoflurane intervention can activate TLR3 and TLR9 signaling pathway, upregulate TLR3 expression and downstream TRIF expression, decrease TLR9 expression, and downregulate downstream NF-κB expression in CPB rat models, thereby mitigating brain injury induced by inflammatory response during CPB.

Post-Traumatic Epilepsy and Seizure Susceptibility in Rat Models of Penetrating and Closed-Head Brain Injury

2020 ◽  
Vol 37 (2) ◽  
pp. 236-247
Author(s):  
Xi-Chun M. Lu ◽  
Jenny Browning ◽  
Zhilin Liao ◽  
Ying Cao ◽  
Weihong Yang ◽  
...  
Keyword(s):  
Brain Injury ◽  
Seizure Susceptibility ◽  
Rat Models ◽  
Closed Head ◽  
Post Traumatic ◽  
Post Traumatic Epilepsy ◽  
Traumatic Epilepsy

MicroRNAs as Novel Biomarkers for the Diagnosis and Prognosis of Mild and Severe Traumatic Brain Injury

2017 ◽  
Vol 34 (11) ◽  
pp. 1948-1956 ◽  
Author(s):  
Valentina Di Pietro ◽  
Marco Ragusa ◽  
David Davies ◽  
Zhangjie Su ◽  
Jon Hazeldine ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Severe Traumatic Brain Injury ◽  
Diagnosis And Prognosis ◽  
Novel Biomarkers

scholarly journals Plasma Exosome-derived MicroRNAs as Novel Biomarkers of Traumatic Brain Injury in Rats

2020 ◽  
Vol 17 (4) ◽  
pp. 437-448 ◽  
Author(s):  
Pengcheng Wang ◽  
Haoli Ma ◽  
Yuxian Zhang ◽  
Rong Zeng ◽  
Jiangtao Yu ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Novel Biomarkers

scholarly journals Behavioral and brain morphological analysis of non-inflammatory and inflammatory Rat models of preterm brain injury

2021 ◽  
pp. 107540
Author(s):  
Ruben Rocha ◽  
Leonardo Andrade ◽  
Tânia Alves ◽  
Susana Sá ◽  
Pedro A. Pereira ◽  
...  
Keyword(s):  
Brain Injury ◽  
Morphological Analysis ◽  
Rat Models
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