scholarly journals Modification of Interleukin-10 with Mannose-6-Phosphate Groups Yields a Liver-Specific Cytokine with Antifibrotic Activity in Rats

Pharmacology ◽  
10.5772/33440 ◽  
2012 ◽  
Author(s):  
Heni Rachmawati ◽  
Adriana Mattos ◽  
Catharina Reker-Smit ◽  
Klaas Poelstra ◽  
Leonie Beljaars
Keyword(s):  
Interleukin 10 ◽  
Mannose 6 Phosphate ◽  
Phosphate Groups

Chemical Modification of Interleukin-10 with Mannose 6-Phosphate Groups Yields a Liver-Selective Cytokine

2007 ◽  
Vol 35 (5) ◽  
pp. 814-821 ◽  
Author(s):  
Heni Rachmawati ◽  
Catharina Reker-Smit ◽  
Marjolijn N. Lub-de Hooge ◽  
Annemiek van Loenen-Weemaes ◽  
Klaas Poelstra ◽  
...  
Keyword(s):  
Chemical Modification ◽  
Interleukin 10 ◽  
Mannose 6 Phosphate ◽  
Phosphate Groups

Dextran from Leuconostoc mesenteroides Augments Immunostimulatory Effects by the Introduction of Phosphate Groups

2004 ◽  
Vol 67 (8) ◽  
pp. 1719-1724 ◽  
Author(s):  
TOSHIHIRO SATO ◽  
JUNKO NISHIMURA-UEMURA ◽  
TAKESHI SHIMOSATO ◽  
YASUSHI KAWAI ◽  
HARUKI KITAZAWA ◽  
...  
Keyword(s):  
Polyphosphoric Acid ◽  
Leuconostoc Mesenteroides ◽  
Phosphorus Content ◽  
Interleukin 10 ◽  
Mitogenic Response ◽  
Strong Response ◽  
Murine Splenocytes ◽  
Immunological Effects ◽  
Formamide Solution ◽  
Phosphate Groups

The immunological effects of phosphorylated dextran (in which phosphate groups were chemically introduced) on murine splenocytes were examined. When dextran produced by Leuconostoc mesenteroides was phosphorylated by a reaction with polyphosphoric acid in formamide solution for 48 h, the degree of phosphorylation of dextran was maximal. The highest phosphorus content (1.7%, wt/wt) was observed in 40 kDa of dextran. The mitogenic response of murine splenocytes was enhanced by the phosphorylated dextran, but its activity was not related to its molecular weight. A strong response was detected at a concentration of 10 to 500 μg/ml, and the highest activity was obtained 48 h after stimulation. Phosphorylated dextran was characterized as a B-cell–specific mitogen. The expressions of CD86 on CD8α−CD11c− and CD8α−CD11c+ cells were augmented by phosphorylated dextran. The levels of mRNA expression of gamma interferon and interleukin-10 on murine splenocytes were also increased by the stimulation. These results demonstrate that dextran exerts immunostimulation by the introduction of phosphate groups.

Ultrastructural localization of specific nucleoprotein fractions

1978 ◽  
Vol 36 (2) ◽  
pp. 204-205
Author(s):  
G. L. Brown
Keyword(s):  
Gel Electrophoresis ◽  
Mouse Liver ◽  
Polyacrylamide Gel Electrophoresis ◽  
Ultrastructural Localization ◽  
Polyacrylamide Gel ◽  
Acid Extraction ◽  
Acid Solutions ◽  
Low Salt ◽  
Liver Nuclei ◽  
Phosphate Groups

Bismuth (Bi) stains nucleoproteins (NPs) by interacting with available amino and primary phosphate groups. These two staining mechanisms are distinguishable by glutaraldehyde crosslinking (Fig. 1,2).Isolated mouse liver nuclei, extracted with salt and acid solutions, fixed in either formaldehyde (form.) or gl utaraldehyde (glut.) and stained with Bi, were viewed to determine the effect of the extractions on Bi stainina. Solubilized NPs were analyzed by SDS-polyacrylamide gel electrophoresis.Extraction with 0.14 M salt does not change the Bi staining characteristics (Fig. 3). 0.34 M salt reduces nucleolar (Nu) staining but has no effect on interchromatinic (IC) staining (Fig. 4). Proteins responsible for Nu and glut.- insensitive IC staining are removed when nuclei are extracted with 0.6 M salt (Fig. 5, 6). Low salt and acid extraction prevents Bi-Nu staining but has no effect on IC staining (Fig. 7). When nuclei are extracted with 0.6 M salt followed by low salt and acid, all Bi-staining components are removed (Fig. 8).

Interleukin 10-induced thrombocytopenia in normal healthy adult volunteers: evidence for decreased platelet production

2000 ◽  
Vol 111 (1) ◽  
pp. 104-111 ◽  
Author(s):  
Jeffrey A. Sosman ◽  
Amit Verma ◽  
Steven Moss ◽  
Patricia Sorokin ◽  
Michael Blend ◽  
...  
Keyword(s):  
Healthy Adult ◽  
Interleukin 10 ◽  
Platelet Production ◽  
Adult Volunteers
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