scholarly journals Molecular Characterization and Serotyping of Listeria monocytogenes with a Focus on Food Safety and Disease Prevention

10.5772/33009 ◽  
2012 ◽  
Author(s):  
I.C. Morobe ◽  
C.L. Obi ◽  
M.A. Nyila ◽  
M.I. Matsheka ◽  
B.A. Gashe
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Disease Prevention ◽  
Molecular Characterization

Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans

Food Control ◽  
2016 ◽  
Vol 70 ◽  
pp. 96-102 ◽  
Author(s):  
Xudong Su ◽  
Jianmin Zhang ◽  
Weimin Shi ◽  
Xiaowei Yang ◽  
Yong Li ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility

scholarly journals Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011–2014

2019 ◽  
Vol 79 ◽  
pp. 116-122 ◽  
Author(s):  
Ivana Zuber ◽  
Brankica Lakicevic ◽  
Ariane Pietzka ◽  
Dubravka Milanov ◽  
Vesna Djordjevic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Small Scale

scholarly journals An overview of Listeria monocytogenes contamination in ready to eat meat, dairy and fishery foods

2017 ◽  
Vol 47 (2) ◽  
Author(s):  
Carla Susana Rodrigues ◽  
Cláudia Valéria Gonçalves Cordeiro de Sá ◽  
Cristiano Barros de Melo
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Sodium Chloride ◽  
Food Safety ◽  
Pregnant Women ◽  
Foodborne Pathogen ◽  
Chloride Content ◽  
The Elderly ◽  
Fishery Products ◽  
Serious Disease

ABSTRACT: Listeria monocytogenes is a relevant foodborne pathogen in public health, responsible for outbreaks of listeriosis often associated to the consumption of ready to eat meat, dairy and fishery products. Listeriosis is a serious disease that can lead to death and mainly affect children, the elderly and immunocompromised individuals. In pregnant women causes abortion or neonatal listeriosis. In Brazil, ready to eat food are appreciated and increasingly consumed by the population. Furthermore, products such as sausages, bologna, hams and cheeses have characteristics such as pH, Aw and sodium chloride content that favor the development of L. monocytogenes during their shelf life. The purpose of this paper was to present an overview of L. monocytogenes contamination in different meat, dairy and fishery products that are ready for consumption and thereby support the adoption of strategies to mitigate this risk, contributing to achieve the appropriate level protection for the consumers and thus strengthen Brazil's food safety system.

scholarly journals RAPID LISTERIA MONOCYTOGENES ASSAY BASED ON HELICASE DEPENDENT AMPLIFICATION (HDA) AND NUCLEIC ACID HYBRIDIZATION IN BLUE SILVER NANOPLATES

2017 ◽  
Vol 5 (10) ◽  
pp. 322-335
Author(s):  
Kankanit Pisamayarom ◽  
Piyasak Chaumpluk
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety ◽  
Nucleic Acid ◽  
Foodborne Pathogen ◽  
Health And Safety ◽  
Nucleic Acid Hybridization ◽  
Blue Color ◽  
Monitoring Method ◽  
Silver Nanoplates ◽  
Laboratory Facilities

Listeria monocytogenes, a foodborne pathogen, is considered as one of the major problems in food safety. With strong safety regulations, a monitoring measure is essential for protecting the health and safety of consumers. Thus, a reliable monitoring method is required. In this study, a rapid assay based on a combination of helicase dependent amplification (HDA) and DNA signal detection via nucleic acid hybridization in blue silver nanoplates (AgNPls) was established. The assay started directly after short term enrichment in terrific broth using cotton ball swapping technique on seafood surface. A HDA amplification of hly gene of L. monocytogenes at 65 °C allowed DNA signals to be increased, whereas the rendered DNA products were detected via nucleic acid hybridization with an oligonucleotide probe in AgNPls solution. The positive specimens induced blue silver nanoplates’ aggregation resulting in pale gray change to colorless, while the negative specimens showed the blue color of non-aggregated nanoplates. The method had a detection limit at 100 copies of L. monocytogenes DNA per 50 g of sample. This method was rapid, simple, did not require laboratory facilities and was suitable for field food safety monitoring

scholarly journals Genome Sequence of theListeria monocytogenesFood Isolate HPB913, Collected in Canada in 1993

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Arthur W. Pightling ◽  
Hugh Rand ◽  
Errol Strain ◽  
Franco Pagotto
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Food Safety ◽  
Genome Sequence ◽  
Sporadic Case ◽  
Foodborne Illness ◽  
Pathogenic Bacterium ◽  
Food Isolate ◽  
Serotype 1

Listeria monocytogenesis a pathogenic bacterium of importance to public health and food safety agencies. We present the genome sequence of the serotype 1/2aL. monocytogenesfood isolate HPB913, which was collected in Canada in 1993 as part of an investigation into a sporadic case of foodborne illness.

scholarly journals Public health impact of foodborne exposure to naturally occurring virulence-attenuated Listeria monocytogenes: inference from mouse and mathematical models

2019 ◽  
Vol 10 (1) ◽  
pp. 20190046 ◽  
Author(s):  
Alison Stout ◽  
Anna Van Stelten-Carlson ◽  
Hélène Marquis ◽  
Michael Ballou ◽  
Brian Reilly ◽  
...  
Keyword(s):  
Public Health ◽  
Listeria Monocytogenes ◽  
Food Safety ◽  
Protective Immunity ◽  
Health Benefit ◽  
Intestinal Barrier ◽  
Population Level ◽  
T Cell Response ◽  
Infection Model ◽  
Safety Testing

Listeriosis is a clinically severe foodborne disease caused by Listeria monocytogenes (Lm). However, approximately 45% of Lm isolates in food carry a virulence-attenuating single-nucleotide polymorphism in inlA , which normally facilitates crossing the intestinal barrier during the initial stages of infection. We hypothesized that (i) natural exposure to virulence-attenuated (vA) Lm strains through food can confer protective immunity against listeriosis attributable to fully virulent (fV) strains and (ii) current food safety measures to minimize exposure to both Lm strains may have adverse population-level outcomes. To test these hypotheses, we evaluated the host response to Lm in a mouse infection model and through mathematical modelling in a human population. After oral immunization with a murinized vA Lm strain, we demonstrated the elicitation of a CD8+ T-cell response and protection against subsequent challenge with an fV strain. A two-strain compartmental mathematical model of human exposure to Lm with cross-protective immunity was also developed. If food safety testing strategies preferentially identify and remove food contaminated by vA strains (potentially due to their common occurrence in foods and higher concentration in food compared to fV strains), the model predicted minimal public health benefit to potentially adverse effects. For example, reducing vA exposures by half, while maintaining fV exposures results in an approximately 6% rise in annual incidence.

Factors contributing to Listeria monocytogenes transmission and impact on food safety

2020 ◽  
Vol 36 ◽  
pp. 9-17
Author(s):  
Birgitte Kallipolitis ◽  
Cormac GM Gahan ◽  
Pascal Piveteau
Keyword(s):  
Listeria Monocytogenes ◽  
Food Safety
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