Prevalence, molecular characterization, genetic heterogeneity and antimicrobial resistance of Listeria monocytogenes associated with fish and fishery environment in Kerala, India

2019 ◽  
Vol 69 (4) ◽  
pp. 286-293 ◽  
Author(s):  
K.A. Basha ◽  
N.R. Kumar ◽  
V. Das ◽  
K. Reshmi ◽  
B.M. Rao ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Genetic Heterogeneity

Listeria monocytogenes in raw milk, milking equipment and dairy workers: Molecular characterization and antimicrobial resistance patterns

2017 ◽  
Vol 10 ◽  
pp. 264-270 ◽  
Author(s):  
Asmaa B.M.B. Tahoun ◽  
Rasha M.M. Abou Elez ◽  
Eman N. Abdelfatah ◽  
Ibrahim Elsohaby ◽  
Attia A. El-Gedawy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Raw Milk ◽  
Dairy Workers ◽  
Resistance Patterns

scholarly journals Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

2019 ◽  
Vol 8 (1) ◽  
pp. 18 ◽  
Author(s):  
Larrayane A.C. Teixeira ◽  
Fernanda T. Carvalho ◽  
Deyse C. Vallim ◽  
Rodrigo C.L. Pereira ◽  
Adelino Cunha Neto ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Multiplex Pcr ◽  
Molecular Characterization ◽  
Sodium Hypochlorite ◽  
High Resistance ◽  
Molecular Properties ◽  
Mato Grosso ◽  
Microbiological Safety

The Brazilian state of Mato Grosso is the largest producer and exporter of beef in the country, but few studies of relevance have been conducted to evaluate the microbiological safety of its products. This study aimed to estimate the prevalence of Listeria monocytogenes (LM) in export-approved beef from Mato Grosso and to characterize the isolates in terms of molecular properties and antimicrobial resistance. From a total of 50 samples analyzed, Listeria sp. was isolated in 18 (36% prevalence). Listeria monocytogenes was confirmed in 6 (12% prevalence). Among the serotype groups assessed by multiplex PCR, serotype 4 (4b, 4d or 4e) was the most prevalent. Although antibiotic resistance was not an issue, two strains isolated from different plants showed high resistance to sodium hypochlorite. Overall, this scenario causes concern because it puts at risk not only the Brazilian customer, but also the population of countries that import beef from Mato Grosso.

Molecular characterization and antimicrobial resistance profiles of Salmonella Heidelberg isolates from poultry

2021 ◽  
Author(s):  
Johana Elizabeth Dominguez ◽  
María Rosa Viñas ◽  
Mariana Herrera ◽  
Mirian Moroni ◽  
Gabriel Omar Gutkind ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Salmonella Heidelberg

scholarly journals Molecular Characterization of Antimicrobial Resistance and Virulence Genes of Bacterial Pathogens from Bovine and Caprine Mastitis in Northern Lebanon

2021 ◽  
Vol 9 (6) ◽  
pp. 1148
Author(s):  
Zahie Abboud ◽  
Lucia Galuppo ◽  
Marco Tolone ◽  
Maria Vitale ◽  
Roberto Puleio ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility ◽  
Meca Gene ◽  
Toxin Gene ◽  
Pcr Screening ◽  
E Coli ◽  
Presenting Symptoms ◽  
Flight Mass Spectrometry ◽  
Streptococcus Uberis

Mastitis is an infectious disease encountered in dairy animals worldwide that is currently a growing concern in Lebanon. This study aimed at investigating the etiology of the main mastitis-causing pathogens in Northern Lebanon, determining their antimicrobial susceptibility profiles, and identifying their antimicrobial resistance (AMR) genes. A total of 101 quarter milk samples were collected from 77 cows and 11 goats presenting symptoms of mastitis on 45 dairy farms. Bacterial identification was carried out through matrix-assisted laser desorption/ionization-time of flight mass spectrometry. Antimicrobial susceptibility was tested by disc diffusion and broth microdilution methods. Molecular characterization included polymerase chain reaction (PCR) screening for genes encoding extended-spectrum beta-lactamases (ESBLs) and plasmid-mediated AmpC among Enterobacterales isolates, and virulence factors among Staphylococcus isolates. Escherichia coli isolates were subjected to phylogenetic typing by a quadruplex PCR method. The most frequently identified species were Streptococcus uberis (19.2%), Streptococcus agalactiae (15.1%), E. coli (12.3%), and Staphylococcus aureus (10.96%). Gram-positive bacteria were resistant to macrolides and tetracycline, whereas gram-negative bacteria displayed resistance to ampicillin and tetracycline. Two ESBL genes, blaTEM (83.3%) and blaOXA (16.7%), and one AmpC beta-lactamase gene, blaCMY-II (16.7%), were detected among six E. coli isolates, which mainly belonged to phylogenetic group B1. Among Staphylococcus spp., the mecA gene was present in three isolates. Furthermore, four isolates contained at least one toxin gene, and all S. aureus isolates carried the ica operon. These findings revealed the alarming risk of AMR in the Lebanese dairy chain and the importance of monitoring antimicrobial usage.

Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans

Food Control ◽  
2016 ◽  
Vol 70 ◽  
pp. 96-102 ◽  
Author(s):  
Xudong Su ◽  
Jianmin Zhang ◽  
Weimin Shi ◽  
Xiaowei Yang ◽  
Yong Li ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Antimicrobial Susceptibility

scholarly journals 1667. Change in characteristics of community-onset ciprofloxacin-resistant E. coli isolates causing community-acquired acute pyelonephritis in South Korea

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S820-S820
Author(s):  
Bongyoung Kim ◽  
Ki Tae Kwon ◽  
Seong-yeol Ryu ◽  
Seong-Heon Wie ◽  
Hyun-uk Jo ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
South Korea ◽  
Molecular Characterization ◽  
Acute Pyelonephritis ◽  
Multilocus Sequence Typing ◽  
Quinolone Resistance ◽  
E Coli ◽  
Extended Spectrum ◽  
Community Onset

Abstract Background The aim of this study was to examine the change in characteristics of community-onset ciprofloxacin-resistant (CIP-R) E. coli isolates causing community-acquired acute pyelonephritis (CA-APN) in South Korea between 2010-2011 and 2017-2018. Methods E. coli samples isolated from the blood or urine were collected from patients with CA-APN aged 19 years and more who were admitted to 8 Korean hospitals from September 2017 to August 2018, prospectively. One isolate was collected from each patient. Phylogenetic typing, multilocus sequence typing (MLST), and molecular characterization of β-lactamase resistance and plasmid-mediated quinolone resistance (PMQR) determinants were performed. The data were compared with those from the previous study with same design in 2010-2011. Results A total of 346 and 300 isolates were collected during 2017-2018 and 2010-2011, respectively. Among them, 76 (22.0%) and 77 (25.7%) were CIP-R isolates. Significantly higher antimicrobial resistance against ampicillin (75.7% vs. 100%, P < 0.001) and cefotaxime (23.9% vs. 77.9%, P < 0.001) were observed for isolates in 2017-2018 compared to those in 2010-2011. The proportion of phylogenic group B2 had increased significantly (44.7% vs. 79.2%, P < 0.001). As for MLST, the proportion of ST131 (27.6% vs. 66.2%, P < 0.001) had increased while that of ST393 (18.4% vs. 3.9%, P =0.004) had decreased significantly. Higher proportion of CIP-R E. coli isolates in 2017-2018 had extended-spectrum β-lactamase (ESBL)/plasmid-mediated AmpC β-lactamase (PABL) (23.7% vs. 79.2%, P < 0.001) and PMQR determinant (11.8% vs. 40.8%, P < 0.001) compared to those in 2010-2011. Phlogenetic tree Analyzed by SplitsTree Conclusion Among uropathogenic CIP-R E. coli isolates in South Korea, ST131 predominance had become more prominent and the proportion of containing ESBL/PABL and/or PMQR determinants had increased. Disclosures All Authors: No reported disclosures

scholarly journals Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011–2014

2019 ◽  
Vol 79 ◽  
pp. 116-122 ◽  
Author(s):  
Ivana Zuber ◽  
Brankica Lakicevic ◽  
Ariane Pietzka ◽  
Dubravka Milanov ◽  
Vesna Djordjevic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Small Scale
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