scholarly journals Utilization of Aspergillus niger Phytase Preparation for Hydrolysis of Phytate in Foods

Food Additive ◽  
10.5772/32294 ◽  
2012 ◽  
Author(s):  
Akiko Matsuo ◽  
Kenji Sato
2005 ◽  
Vol 58 (5) ◽  
pp. 267-272 ◽  
Author(s):  
Akiko Matsuo ◽  
Kenji Sato ◽  
Yasushi Nakamura ◽  
Kozo Ohtsuki

Processes ◽  
2021 ◽  
Vol 9 (8) ◽  
pp. 1360
Author(s):  
Ekaterina Budenkova ◽  
Stanislav Sukhikh ◽  
Svetlana Ivanova ◽  
Olga Babich ◽  
Vyacheslav Dolganyuk ◽  
...  

Enzymatic hydrolysis of cellulose-containing raw materials, using Aspergillus niger, were studied. Filter paper, secondary cellulose-containing or starch-containing raw materials, miscanthus cellulose after alkaline or acid pretreatment, and wood chip cellulose, were used as substrates. The study focused on a wild A. niger strain, treated, or not (control), by ultraviolet (UV) irradiations for 45, 60, or 120 min (UV45, UV60, or UV120), or by UV irradiation for 120 min followed by a chemical treatment with NaN3 + ItBr for 30 min or 80 min (UV120 + CH30 or UV120 + CH80). A mixture of all the A. niger strains (MIX) was also tested. A citrate buffer, at 50 mM, wasthe most suitable for enzymatic hydrolysis. As the UV exposure time increased to 2 h, the cellulase activity of the surviving culturewas increased (r = 0.706; p < 0.05). The enzymatic activities of the obtained strains, towards miscanthus cellulose, wood chips, and filter paper, were inferior to those obtained with commercial enzymes (8.6 versus 9.1 IU), in some cases. Under stationary hydrolysis at 37 °C, pH = 4.7, the enzymatic activity of A. niger UV120 + CH30 was 24.9 IU. The enzymatic hydrolysis of secondary raw materials, using treated A. niger strains, was themost effective at 37 °C. Similarly, the most effective treatment of miscanthus cellulose and wood chips occurred at 50 °C. The maximum conversion of cellulose to glucose was observed using miscanthus cellulose (with alkaline pretreatment), and the minimum conversion was observed when using wood chips. The greatest value of cellulase activity was evidenced in the starch-containing raw materials, indicating that A. niger can ferment not only through cellulase activity, but also via an amylolytic one.


1992 ◽  
Vol 55 (3) ◽  
pp. 425-430 ◽  
Author(s):  
S. Beers ◽  
A. W. Jongbloed

AbstractA total of 384 piglets from a three-way cross Yorkshire ♂ × (Finnish Landrace ♂ × Dutch Landrace♀) ♀ in the live-weight range of 11 to 25 kg were used in a feeding trial. The effect of supplementary microbial phytase from Aspergillus niger var. Van Tieghem on performance (average daily gain (ADG), average daily food intake (ADFI) and food conversion ratio (FCR)) and apparent digestibility of total phosphorus (P), in comparison with a non-supplemented basal diet or a diet supplemented with one or two levels of monocalcium phosphate (MCP), were studied.The piglets were allotted to 12 blocks. Within each block, four experimental diets were offered ad libitum for a period of 4 weeks i.e. (1) basal diet; (2) basal diet + 1·0 g P, from MCP per kg diet; (3) basal diet + 1·9 g P, from MCP per kg diet; and (4) basal diet + 1450 phytase units (PTU) per kg diet. The diets, with added chromium III oxide as a marker, were cold pelleted without steam at a temperature not above 60°C to avoid a possible inactivation of the phytase. In all diets the ratio calcium: digestible P was intended to be constant.Adding 1450 PTU of Aspergillus niger phytase enhanced the apparent digestibility of P by proportionately 0·216 when compared with diet 1 and by proportionately 0·100 compared with diet 3. In addition, significantly higher ADG and ADFI and better FCR were noted in pigs receiving supplementary microbial phytase than in pigs on any other treatment. The content of digestible P of the diet with added phytase, however, zvas between the two levels of the diets with added MCP.


1995 ◽  
Vol 47 (3) ◽  
pp. 219-228 ◽  
Author(s):  
F. Schöne ◽  
H. Lüdke ◽  
U. Kirchheim

2019 ◽  
Vol 46 (2) ◽  
pp. 161-169
Author(s):  
Marija Ćorović ◽  
Milica Simović ◽  
Ana Milivojević ◽  
Katarina Banjanac ◽  
Katarina Katić ◽  
...  

2018 ◽  
Vol 5 (4) ◽  
Author(s):  
Caio C. Aragon ◽  
Ana I. Ruiz-Matute ◽  
Nieves Corzo ◽  
Rubens Monti ◽  
Jose M. Guisán ◽  
...  

1991 ◽  
Vol 13 (1) ◽  
pp. 87-90 ◽  
Author(s):  
V. Kéry ◽  
G. Kogan ◽  
K. Zajacová ◽  
K. Slámová ◽  
L. Masler ◽  
...  

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