scholarly journals Molecular Pathogenesis of Prion Diseases

10.5772/30952 ◽  
2012 ◽  
Author(s):  
Giuseppe Legname ◽  
Gianluigi Zanusso
Keyword(s):  
Prion Diseases ◽  
Molecular Pathogenesis

scholarly journals Recent advances on the molecular pathogenesis of prion diseases

2019 ◽  
Vol 29 (2) ◽  
pp. 245-247
Author(s):  
Markus Glatzel ◽  
Christina J. Sigurdson
Keyword(s):  
Prion Diseases ◽  
Molecular Pathogenesis ◽  
Recent Advances

scholarly journals Molecular pathogenesis of sporadic prion diseases in man

Prion ◽  
2012 ◽  
Vol 6 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Jiri G. Safar
Keyword(s):  
Prion Diseases ◽  
Molecular Pathogenesis

Molecular pathogenesis of hereditary inclusion body myopathies

2009 ◽  
Vol 40 (01) ◽  
Author(s):  
S Krause ◽  
N Garcia-Angarita ◽  
A Aleo ◽  
S Hinderlich ◽  
MC Walter ◽  
...  
Keyword(s):  
Inclusion Body ◽  
Molecular Pathogenesis

Advances in molecular pathogenesis thyroid cancer: therapeutic implications

2014 ◽  
Author(s):  
Pilar Santisteban ◽  
Ana Sastre-Perona ◽  
Leon Wert-Lamas ◽  
Garcilaso Riesco-Eizaguirre
Keyword(s):  
Thyroid Cancer ◽  
Molecular Pathogenesis ◽  
Therapeutic Implications

Rapid Quantification of Prion Proteins

2019 ◽  
Author(s):  
Matthew Healey ◽  
Muttuswamy Sivakumaran ◽  
Mark Platt
Keyword(s):  
Prion Proteins ◽  
Prion Diseases ◽  
Sample Volume ◽  
Cellular Prion Protein ◽  
Dna Aptamer ◽  
Superparamagnetic Beads ◽  
Resistive Pulse ◽  
Large Sample Volume ◽  
Complex Sample ◽  
Neurological Conditions

<p>Prion diseases are a group of fatal transmissible neurological conditions caused by the change in conformation of the normal intrinsic cellular prion protein (PrP<sup>C</sup>) in to the highly ordered insoluble amyloid state conformer (PrP<sup>SC</sup>). We present a rapid assay using Aptamers and Resistive Pulse Sensing, RPS, to extract and quantify proteins from complex sample matrices, demonstrate with the quantification of PrP<sup>c</sup>. We functionalise the surface of superparamagnetic beads, SPBs, with a DNA aptamer. First SPB’s termed P-Beads, are used to pre-concentrate the analyte from a large sample volume. The PrP<sup>c</sup> protein is then eluted from the P-Beads before aptamer modified sensing beads, S-Beads, are added. The velocity of the S-Beads through the nanopore reveals the concentration of the PrP<sup>c</sup> protein. The process is done in under an hour and allows the detection of picomol’s of protein. The technique could be easily adopted to the mutated version of the protein and integrated into clinical workflows for the screening of blood donations and transfusions. </p>

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