scholarly journals Exogenous Catalase Gene Expression as a Tool for Enhancing Metabolic Activity and Production of Biomaterials in Host Microorganisms

10.5772/29765 ◽  
2012 ◽  
Author(s):  
Ahmad Iskandar Bin Haji Mohd Taha ◽  
Hidetoshi Okuyama ◽  
Takuji Ohwada ◽  
Isao Yumoto ◽  
Yoshitake Orikas
Keyword(s):  
Gene Expression ◽  
Metabolic Activity ◽  
Catalase Gene

scholarly journals Influence of Angiopoietin Treatment with Hypoxia and Normoxia on Human Intervertebral Disc Progenitor Cell’s Proliferation, Metabolic Activity, and Phenotype

2021 ◽  
Vol 11 (15) ◽  
pp. 7144
Author(s):  
Muriel C. Bischof ◽  
Sonja Häckel ◽  
Andrea Oberli ◽  
Andreas S. Croft ◽  
Katharina A. C. Oswald ◽  
...  
Keyword(s):  
Gene Expression ◽  
Intervertebral Disc ◽  
Metabolic Activity ◽  
Cell Metabolism ◽  
Trauma Patients ◽  
Low Back ◽  
Relative Gene Expression ◽  
Progenitor Cell Population ◽  
Ivd Degeneration ◽  
Relative Gene

Increasing evidence implicates intervertebral disc (IVD) degeneration as a major contributor to low back pain. In addition to a series of pathogenic processes, degenerated IVDs become vascularized in contrast to healthy IVDs. In this context, angiopoietin (Ang) plays a crucial role and is involved in cytokine recruitment, and anabolic and catabolic reactions within the extracellular matrix (ECM). Over the last decade, a progenitor cell population has been described in the nucleus pulposus (NP) of the IVD to be positive for the Tie2 marker (also known as Ang-1 receptor). In this study, we investigated the influence of Ang-1 and Ang-2 on human NP cell (Tie2+, Tie2- or mixed) populations isolated from trauma patients during 7 days in normoxia (21% O2) or hypoxia (≤ 5% O2). At the end of the process, the proliferation and metabolic activity of the NP cells were analyzed. Additionally, the relative gene expression of NP-related markers was evaluated. NP cells showed a higher proliferation depending on the Ang treatment. Moreover, the study revealed higher NP cell metabolism when cultured in hypoxia. Additionally, the relative gene expression followed, with an increase linked to the oxygen level and Ang concentration. Our study comparing different NP cell populations may be the start of new approaches for the treatment of IVD degeneration.

scholarly journals Biotribological Tests of Osteochondral Grafts after Treatment with Pro-Inflammatory Cytokines

Cartilage ◽  
2021 ◽  
pp. 194760352199490
Author(s):  
Christoph Bauer ◽  
Hakan Göçerler ◽  
Eugenia Niculescu-Morzsa ◽  
Vivek Jeyakumar ◽  
Christoph Stotter ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Cytokines ◽  
Metabolic Activity ◽  
Wear Debris ◽  
Cartilage Tissue ◽  
Test Fluid ◽  
Osteochondral Grafts ◽  
Extracellular Matrix Components ◽  
Proteoglycan Content ◽  
Pro Inflammatory Cytokines

ObjectiveDuring osteoarthritis progression, cartilage degrades in a manner that influences its biomechanical and biotribological properties, while chondrocytes reduce the synthesis of extracellular matrix components and become apoptotic. This study investigates the effects of inflammation on cartilage under biomechanical stress using biotribological tests.MethodsBovine osteochondral grafts from five animals were punched out from the medial condyle and treated with or without pro-inflammatory cytokines (interleukin-1β [IL-1β], tumor necrosis factor-α [TNF-α], IL-6) for 2 weeks. After incubation, biotribological tests were performed for 2 hours (alternating 10 minutes test and pause respectively at 39°C, 180 N, 1 Hz, and 2 mm stroke). Before and after testing, the cartilage surface was imaged with a 3-dimensional microscope. During testing, the coefficient of friction (COF) was measured, while gene expression analysis and investigation of metabolic activity of chondrocytes were carried out after testing. Histological sections of the tissue and wear debris from the test fluid were also analyzed.ResultsAfter biotribological tests, surface cracks were found in both treated and untreated osteochondral grafts. In treated grafts, the COF increased, and the proteoglycan content in the cartilage tissue decreased, leading to structural changes. Chondrocytes from treated grafts showed increased expression of genes encoding for degradative enzymes, while cartilage-specific gene expression and metabolic activity exhibited no significant differences between treated and untreated groups. No measurable difference in the wear debris in the test fluid was found.ConclusionsTreatment of osteochondral grafts with cytokines results in a significantly increased COF, while also leading to significant changes in cartilage proteoglycan content and cartilage matrix compression during biotribological tests.

scholarly journals Immune deposit and vasculopathy in metabolic-active lung tissues of patients with pulmonary tuberculosis

2021 ◽  
Author(s):  
Hui Ma ◽  
Lin Wang ◽  
Zilu Wen ◽  
Xinchun Chen ◽  
Haiying Liu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Systemic Lupus Erythematosus ◽  
Inflammatory Response ◽  
Pulmonary Tuberculosis ◽  
Autoimmune Diseases ◽  
Metabolic Activity ◽  
Lupus Erythematosus ◽  
Immune Complexes ◽  
Systemic Lupus ◽  
Dsdna Antibodies

ABSTRACTMetabolic activity in pulmonary lesion is associated with disease severity and relapse risk in tuberculosis. However, the nature of the metabolic activity associated with tuberculosis in humans remains unclear. Previous works indicate that tuberculosis bears resemblance transcriptionally with systemic lupus erythematosus in peripheral blood, except that the plasma cell component was absent in tuberculosis. Here we reported that the missing transcriptional component was present within the metabolic active tissues in the lung of patients with sputum culture-negative tuberculosis, within which increased levels of circulating immune complexes and anti-dsDNA antibodies were found relative to nearby non-metabolic active tissues. Histological examination revealed specific vascular deposition of immune complexes, neutrophil extracellular traps, and vascular necrosis in the metabolic-active tissue. Thus, tuberculosis-initiated metabolic activity was associated with hyperactive antibody responses and vascular pathology, and shared features with systemic lupus erythematosus and other autoimmune diseases. We discussed these observations in the context of earlier literatures demonstrating that similar effects could be induced in humans and animal models by complete freund’s adjuvant, the most potent antibody response inducer ever reported. Our small case series, if verified in a larger size study, might help inform host-directed therapies to alleviate disease progression and augment treatment efficacy.IMPORTANCEIn patients with pulmonary tuberculosis, lung tissues were destroyed by a hyperactive inflammatory response towards M. tuberculosis. The mechanisms underlying the inflammatory response are still poorly understood. Using 18F-FDG avidity as a surrogate marker of inflammation, we have identified that hyper-inflamed tissues possessed features associated with systemic lupus erythematosus: gene expression signatures of plasma cell and immunoglobulins and increased levels of anti-dsDNA antibodies, immune deposits, and vasculopathy. This observation might suggest an explanation to why patients with tuberculosis share more gene expression signatures with autoimmune diseases than infectious diseases and why they are more likely to develop autoimmune diseases. Defining the inflammatory responses at the lesion could help inform host-directed therapies to intervene disease progression or even accelerate cure.

Regulation of catalase gene expression by copper.

1992 ◽  
Vol 47 (3-4) ◽  
pp. 52
Author(s):  
P.J. Lapinskas ◽  
V. Culotta
Keyword(s):  
Gene Expression ◽  
Catalase Gene

Alpha-lipoic acid prevents arsenic toxicity in HepG2 cells by upregulation of GSH content, metabolic activity and HOX1 gene expression

2007 ◽  
Vol 172 ◽  
pp. S47-S48
Author(s):  
Selene Huerta-Olvera ◽  
José Macías-Barragán ◽  
Juan Armendáriz-Borunda ◽  
Fernando Diaz-Barriga ◽  
Fernando Siller-López
Keyword(s):  
Gene Expression ◽  
Metabolic Activity ◽  
Lipoic Acid ◽  
Hepg2 Cells ◽  
Arsenic Toxicity ◽  
Alpha Lipoic Acid

scholarly journals Thymoquinone Upregulates Catalase Gene Expression and Preserves the Structure of the Renal Cortex of Propylthiouracil-Induced Hypothyroid Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-15
Author(s):  
Nasra Ayuob ◽  
Maha Jameal Balgoon ◽  
Ahmed A. El-Mansy ◽  
Wafaa A. Mubarak ◽  
Alaa El-Din L. Firgany
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Protective Effect ◽  
Renal Cortex ◽  
Nigella Sativa ◽  
Serum Levels ◽  
Renal Tissue ◽  
Renal Diseases ◽  
Catalase Gene ◽  
Male Wistar Rats

Background. The association between hypothyroidism and renal diseases has been described in many studies. Nigella Sativa was among the recently reported natural product that has the potential to prevent renal tissue damage and fibrosis. The aim of this study was to evaluate the possible protective effect of thymoquinone on the structure of the renal cortex of hypothyroid rats and explore the mechanism behind it. Methods. An experimental model of hypothyroidism was induced in adult male Wistar rats by administration of propylthiouracil (6 mg/kg/body weight). One hypothyroid group was treated with thymoquinone at the dose of 50 mg/kg/body weight and compared to the untreated group. Thyroid function and oxidant/antioxidant status were assessed in the serum. Catalase gene expression was assessed using the real-time polymerase chain reaction. The kidney was assessed both histologically and immunohistochemically. Results. Administration of propylthiouracil resulted in a significant decrease in the serum levels of nitric oxide, reduced glutathione, and superoxide dismutase activity while the level of malondialdehyde significantly (p<0.001) increased. Administration of thymoquinone alleviated this effect on the thyroid hormones and significantly increased the serum levels of antioxidants. Thymoquinone significantly (p<0.001) upregulated catalase transcription by about 24-fold and could block the hypothyroidism-induced glomerular and tubular injury. Conclusion. Thymoquinone may have a potential protective effect against hypothyroidism-induced renal injury acting through the attenuation of the oxidative stress and upregulation of renal catalase gene expression.

Heme oxygenase and catalase gene expression in nodules and roots of soybean plants subjected to cadmium stress

BioMetals ◽  
2008 ◽  
Vol 21 (4) ◽  
pp. 433-441 ◽  
Author(s):  
Karina B. Balestrasse ◽  
Gustavo G. Yannarelli ◽  
Guillermo O. Noriega ◽  
Alcira Batlle ◽  
Maria L. Tomaro
Keyword(s):  
Gene Expression ◽  
Heme Oxygenase ◽  
Cadmium Stress ◽  
Catalase Gene ◽  
Soybean Plants

scholarly journals Metabolic activity of Streptococcus mutans biofilms and gene expression during exposure to xylitol and sucrose

2014 ◽  
Vol 6 (4) ◽  
pp. 195-204 ◽  
Author(s):  
Eva-Maria Decker ◽  
Christian Klein ◽  
Dimitri Schwindt ◽  
Christiane von Ohle
Keyword(s):  
Gene Expression ◽  
Streptococcus Mutans ◽  
Metabolic Activity

scholarly journals IC-P-072: Gene Expression Of ABCA7 Dysregulated in Peripheral Blood is Associated With Decreased Metabolic Activity in Hippocampus

2016 ◽  
Vol 12 ◽  
pp. P56-P58
Author(s):  
Kwangsik Nho ◽  
Sungeun Kim ◽  
Emrin Horgousluoglu ◽  
Shannon L. Risacher ◽  
Liana G. Apostolova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Activity ◽  
Peripheral Blood
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