Epidermal Serine Proteases and Their Inhibitors in Atopic Dermatitis

Multifaceted Analyses of Epidermal Serine Protease Activity in Patients with Atopic Dermatitis

International Journal of Molecular Sciences ◽

10.3390/ijms21030913 ◽

2020 ◽

Vol 21 (3) ◽

pp. 913 ◽

Cited By ~ 1

Author(s):

Hayato Nomura ◽

Mutsumi Suganuma ◽

Takuya Takeichi ◽

Michihiro Kono ◽

Yuki Isokane ◽

...

Keyword(s):

Atopic Dermatitis ◽

Serine Protease ◽

Protease Activity ◽

Serine Proteases ◽

Immunoglobulin E ◽

Total Serum ◽

Tape Stripping ◽

Blood Eosinophil ◽

Peripheral Blood Eosinophil ◽

Serine Protease Activity

The serine proteases kallikrein-related peptidase (KLK) 5 and KLK7 cleave cell adhesion molecules in the epidermis. Aberrant epidermal serine protease activity is thought to play an important role in the pathogenesis of atopic dermatitis (AD). We collected the stratum corneum (SC) from healthy individuals (n = 46) and AD patients (n = 63) by tape stripping and then measuring the trypsin- and chymotrypsin-like serine protease activity. We also analyzed the p.D386N and p.E420K of SPINK5 variants and loss-of-function mutations of FLG in the AD patients. The serine protease activity in the SC was increased not only in AD lesions but also in non-lesions of AD patients. We found, generally, that there was a positive correlation between the serine protease activity in the SC and the total serum immunoglobulin E (IgE) levels, serum thymus and activation-regulated chemokine (TARC) levels, and peripheral blood eosinophil counts. Moreover, the p.D386N or p.E420K in SPINK5 and FLG mutations were not significantly associated with the SC’s serine protease activity. Epidermal serine protease activity was increased even in non-lesions of AD patients. Such activity was found to correlate with a number of biomarkers of AD. Further investigations of serine proteases might provide new treatments and prophylaxis for AD.

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The role of proteolytic enzymes in the pathogenesis of atopic dermatitis

Nauchno-prakticheskii zhurnal «Patogenez» ◽

10.25557/gm.2017.2.7299 ◽

2017 ◽

pp. 31-36

Author(s):

О.В. Кандалова

Keyword(s):

Atopic Dermatitis ◽

Serine Proteases ◽

Proteolytic Enzymes ◽

Clinical Manifestations ◽

Cysteine Proteases ◽

Protease Activated Receptors

В обзоре приводятся данные, касающиеся роли протеаз всех пяти каталитических классов (сериновых, цистеиновых, треониновых, аспартатных и металлопротеиназ) в патогенезе атопического дерматита (АД). Также рассматриваются протеазо-актвированные рецепторы (PARs) и их роль в клинических проявлениях АД. The review provides data on the role of proteases of all five catalytic classes (serine proteases, cysteine proteases, threonine proteases, aspartate proteases, and metalloproteinases) in the pathogenesis of atopic dermatitis. We also discuss the protease activated receptors (PARs) and its role in the clinical manifestations of atopic dermatitis.

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