scholarly journals Preparation and Characterization Urea-Solubilized Sol-Gel Type I Collagen and Its Possible Use in Applications

10.5772/22117 ◽  
2011 ◽  
Author(s):  
Xin Xiong ◽  
Herwig Brunner ◽  
Robin Ghosh
Keyword(s):  
Type I Collagen ◽  
Sol Gel ◽  
Type I

scholarly journals Organic / inorganic bioactive materials Part I: Synthesis, structure and in vitro assessment of collagen/silicocarnotite biocoatings

2009 ◽  
Vol 7 (4) ◽  
pp. 702-710 ◽  
Author(s):  
Vladimir Hristov ◽  
Lachezar Radev ◽  
Bisserka Samuneva ◽  
Georgi Apostolov
Keyword(s):  
Type I Collagen ◽  
Sol Gel ◽  
Weight Ratio ◽  
Type I ◽  
Bioactive Materials ◽  
Organic Part ◽  
Carboxylate Groups ◽  
Cross Linkage ◽  
Collagen Molecules

AbstractThe silicocarnotite, as an inorganic part of the coatings, has been synthesized using a polystep sol-gel method. The chemical composition of the prepared silicocarnotite sol is described as 58.12 CaO, 29.42 P2O5, 12.45 SiO2 (wt%), where Ca/P+Si = 1,67. The acid soluble type I collagen, as an organic part of the obtained coatings, was mixed with silicocarnotite powder in a weight ratio of 25:75 and 75:25 weight ratio without cross-linkage. The acidity of the obtained mixture was readjust with 25% NH4OH to pH = 9.0. The mixture was then dried at 37°C for 12 h.The growth of B-type carbonate containing hydroxyapatite (B-type CO3HA) in which CO3 2+→PO4 3− on the surface of collagen/silicocarnotite coatings soaked in 1.5 simulated body fluid (1.5 SBF) was observed. The nucleation of B-type CO3HA was estimated on the obtained coatings after 3 days immersion in 1.5 SBF. The negatively charged carboxylate groups from the collagen surface may be responsible for the HA deposition. This was confirmed by the “red shift” of carboxylate groups of collagen molecules in the FTIR spectra. After soaking in 1.5 SBF, the morphology of prepared coatings and HA formation was observed by SEM.

Biomimetic Fabrication and Characterization of BG/COL/HCA Scaffolds for Bone Tissue Engineering

2007 ◽  
Vol 336-338 ◽  
pp. 1574-1576
Author(s):  
Xiao Feng Chen ◽  
Ying Jun Wang ◽  
Chun Rong Yang ◽  
Na Ru Zhao
Keyword(s):  
Tissue Engineering ◽  
Calcium Phosphate ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Porous Scaffold ◽  
Sol Gel ◽  
Type I ◽  
Carbonate Apatite

The bone tissue engineering scaffold was developed by compounded the type I collagen with the porous scaffold of the sol-gel derived bioactive glass (BG) in the system CaO-P2O5-SiO2. The resultant porous scaffold was treated in supersaturated calcification solution (SCS) to form the surface layer of hydroxyl-carbonate-apatite (HCA) since the type I collagen possessed good biocompatibility and bio-absorbability, and also, the ability of inducting calcium phosphates to precipitated inside and outside the collagen fibers where the collagen fibers acted as bio-macromolecules template for formation of bone-like inorganic minerals in nature bone such as: octo-calcium phosphate (OCP), tri-calcium phosphate (TCP) and hydroxyl-carbonate-apatite (HCA). On the other hand, the sol-gel derived bioactive glass also played an important role in formation of the above bio-minerals owing to its serial chemical reactions with the body fluid. The in vitro study in supersaturated calcification solution SCS indicated that the surface of the porous scaffold was able to induce formation of bone-like HCA crystals on the pore walls of the scaffold which possessed satisfactory cells biocompatibility.

Collagen fibrillogenesis in vitro: interaction of types I and V collagen

1986 ◽  
Vol 44 ◽  
pp. 210-211
Author(s):  
Arthur J. Wasserman ◽  
Kathy C. Kloos ◽  
David E. Birk
Keyword(s):  
Type I Collagen ◽  
Corneal Stroma ◽  
Minor Component ◽  
Homogeneous Distribution ◽  
Type I ◽  
Type V Collagen ◽  
Fibril Morphology ◽  
Type V ◽  
In Vitro Interaction

Type I collagen is the predominant collagen in the cornea with type V collagen being a quantitatively minor component. However, the content of type V collagen (10-20%) in the cornea is high when compared to other tissues containing predominantly type I collagen. The corneal stroma has a homogeneous distribution of these two collagens, however, immunochemical localization of type V collagen requires the disruption of type I collagen structure. This indicates that these collagens may be arranged as heterpolymeric fibrils. This arrangement may be responsible for the control of fibril diameter necessary for corneal transparency. The purpose of this work is to study the in vitro assembly of collagen type V and to determine whether the interactions of these collagens influence fibril morphology.

948: Clinical Safety, Histologic Findings and Surgical Methods in Complex Phalloplasty Using Type I Collagen

2007 ◽  
Vol 177 (4S) ◽  
pp. 314-314 ◽  
Author(s):  
Joon-Yang Kim ◽  
Hoon Seog Jean ◽  
Beom Joon Kim ◽  
Kye Yong Song
Keyword(s):  
Type I Collagen ◽  
Type I ◽  
Clinical Safety ◽  
Surgical Methods
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